scholarly journals Synergistic Degradation of Pyrethroids by the Quorum Sensing-Regulated Carboxylesterase of Bacillus subtilis BSF01

2020 ◽  
Vol 8 ◽  
Author(s):  
Ying Xiao ◽  
Qiqi Lu ◽  
Xin Yi ◽  
Guohua Zhong ◽  
Jie Liu
Keyword(s):  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Synergistic Degradation

scholarly journals Peptide signaling without feedback in signal production operates as a true quorum sensing communication system in Bacillus subtilis

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
Iztok Dogsa ◽  
Mihael Spacapan ◽  
Anna Dragoš ◽  
Tjaša Danevčič ◽  
Žiga Pandur ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Cell Density ◽  
Communication System ◽  
Communication Systems ◽  
Feedback Loop ◽  
Signal Molecules ◽  
Specific Cell ◽  
Sharp Transition

AbstractBacterial quorum sensing (QS) is based on signal molecules (SM), which increase in concentration with cell density. At critical SM concentration, a variety of adaptive genes sharply change their expression from basic level to maximum level. In general, this sharp transition, a hallmark of true QS, requires an SM dependent positive feedback loop, where SM enhances its own production. Some communication systems, like the peptide SM-based ComQXPA communication system of Bacillus subtilis, do not have this feedback loop and we do not understand how and if the sharp transition in gene expression is achieved. Based on experiments and mathematical modeling, we observed that the SM peptide ComX encodes the information about cell density, specific cell growth rate, and even oxygen concentration, which ensure power-law increase in SM production. This enables together with the cooperative response to SM (ComX) a sharp transition in gene expression level and this without the SM dependent feedback loop. Due to its ultra-sensitive nature, the ComQXPA can operate at SM concentrations that are 100–1000 times lower than typically found in other QS systems, thereby substantially reducing the total metabolic cost of otherwise expensive ComX peptide.

The 1.2 Å structure of a novel quorum-sensing protein, Bacillus subtilis LuxS 1 1Edited by J. Thornton

2001 ◽  
Vol 313 (1) ◽  
pp. 111-122 ◽  
Author(s):  
S.N Ruzheinikov ◽  
S.K Das ◽  
S.E Sedelnikova ◽  
A Hartley ◽  
S.J Foster ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Quorum Sensing

scholarly journals Self-sensing in Bacillus subtilis quorum-sensing systems

2017 ◽  
Vol 3 (1) ◽  
pp. 83-89 ◽  
Author(s):  
Tasneem Bareia ◽  
Shaul Pollak ◽  
Avigdor Eldar
Keyword(s):  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Sensing Systems

scholarly journals Novel Quorum-Sensing Peptides Mediating Interspecies Bacterial Cell Death

mBio ◽  
2013 ◽  
Vol 4 (3) ◽  
Author(s):  
Sathish Kumar ◽  
Ilana Kolodkin-Gal ◽  
Hanna Engelberg-Kulka
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Cell Death ◽  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Bacterial Cell ◽  
Content Type ◽  
E Coli ◽  
New Class ◽  
Bacterial Cell Death ◽  
Induced Module

ABSTRACTEscherichia colimazEFis a toxin-antitoxin stress-induced module mediating cell death. It requires the quorum-sensing signal (QS) “extracellular death factor” (EDF), the penta-peptide NNWNN (EcEDF), enhancing the endoribonucleolytic activity ofE. colitoxin MazF. Here we discovered thatE. coli mazEF-mediated cell death could be triggered by QS peptides from the supernatants (SN) of the Gram-positive bacteriumBacillus subtilisand the Gram-negative bacteriumPseudomonas aeruginosa. In the SN ofB. subtilis, we found one EDF, the hexapeptide RGQQNE, calledBsEDF. In the SN ofP. aeruginosa, we found three EDFs: the nonapeptide INEQTVVTK, calledPaEDF-1, and two hexadecapeptides, VEVSDDGSGGNTSLSQ, calledPaEDF-2, and APKLSDGAAAGYVTKA, calledPaEDF-3. When added to a dilutedE. colicultures, each of these peptides acted as an interspecies EDF that triggeredmazEF-mediated death. Furthermore, though their sequences are very different, each of these EDFs amplified the endoribonucleolytic activity ofE. coliMazF, probably by interacting with different sites onE. coliMazF. Finally, we suggest that EDFs may become the basis for a new class of antibiotics that trigger death from outside the bacterial cells.IMPORTANCEBacteria communicate with one another via quorum-sensing signal (QS) molecules. QS provides a mechanism for bacteria to monitor each other’s presence and to modulate gene expression in response to population density. Previously, we addedE. coliEDF (EcEDF), the peptide NNWNN, to this list of QS molecules. Here we extended the group of QS peptides to several additional different peptides. The new EDFs are produced by two other bacteria,Bacillus subtilisandPseudomonas aeruginosa. Thus, in this study we established a “new family of EDFs.” This family provides the first example of quorum-sensing molecules participating in interspecies bacterial cell death. Furthermore, each of these peptides provides the basis of a new class of antibiotics triggering death by acting from outside the cell.

scholarly journals Engineering of quorum-sensing systems for improved production of alkaline protease by Bacillus subtilis

2004 ◽  
Vol 96 (3) ◽  
pp. 569-578 ◽  
Author(s):  
H. Tjalsma ◽  
E.J. Koetje ◽  
R. Kiewiet ◽  
O.P. Kuipers ◽  
M. Kolkman ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Alkaline Protease ◽  
Sensing Systems

scholarly journals The RapP-PhrP Quorum-Sensing System of Bacillus subtilis Strain NCIB3610 Affects Biofilm Formation through Multiple Targets, Due to an Atypical Signal-Insensitive Allele of RapP

2014 ◽  
Vol 197 (3) ◽  
pp. 592-602 ◽  
Author(s):  
Shira Omer Bendori ◽  
Shaul Pollak ◽  
Dorit Hizi ◽  
Avigdor Eldar
Keyword(s):  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Response Regulator ◽  
Subtilis Strain ◽  
Transcriptional Level ◽  
Response Regulators ◽  
Multiple Targets ◽  
Content Type ◽  
Negative Effect

The genome ofBacillus subtilis168 encodes eightrap-phrquorum-sensing pairs. Rap proteins of all characterized Rap-Phr pairs inhibit the function of one or several important response regulators: ComA, Spo0F, or DegU. This inhibition is relieved upon binding of the peptide encoded by the cognatephrgene.Bacillus subtilisstrain NCIB3610, the biofilm-proficient ancestor of strain 168, encodes, in addition, therapP-phrPpair on the plasmid pBS32. RapP was shown to dephosphorylate Spo0F and to regulate biofilm formation, but unlike other Rap-Phr pairs, RapP does not interact with PhrP. In this work we extend the analysis of the RapP pathway by reexamining its transcriptional regulation, its effect on downstream targets, and its interaction with PhrP. At the transcriptional level, we show thatrapPandphrPregulation is similar to that of otherrap-phrpairs. We further find that RapP has an Spo0F-independent negative effect on biofilm-related genes, which is mediated by the response regulator ComA. Finally, we find that the insensitivity of RapP to PhrP is due to a substitution of a highly conserved residue in the peptide binding domain of therapPallele of strain NCIB3610. Reversing this substitution to the consensus amino acid restores the PhrP dependence of RapP activity and eliminates the effects of therapP-phrPlocus on ComA activity and biofilm formation. Taken together, our results suggest that RapP strongly represses biofilm formation through multiple targets and that PhrP does not counteract RapP due to a rare mutation inrapP.

Chemical structure and biological activity of a quorum sensing pheromone from Bacillus subtilis subsp. natto

2015 ◽  
Vol 25 (19) ◽  
pp. 4293-4296 ◽  
Author(s):  
Masahiro Okada ◽  
Yuta Nakamura ◽  
Shunsuke Hayashi ◽  
Koki Ozaki ◽  
Syohei Usami
Keyword(s):  
Bacillus Subtilis ◽  
Biological Activity ◽  
Quorum Sensing ◽  
Chemical Structure ◽  
Subtilis Subsp

Structure of the Bacillus subtilis quorum-sensing peptide pheromone ComX

2005 ◽  
Vol 1 (1) ◽  
pp. 23-24 ◽  
Author(s):  
Masahiro Okada ◽  
Isao Sato ◽  
Soo Jeong Cho ◽  
Hidehisa Iwata ◽  
Toshihiko Nishio ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Peptide Pheromone

scholarly journals ComQXPA Quorum Sensing Systems May Not Be Unique to Bacillus subtilis: A Census in Prokaryotic Genomes

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e96122 ◽  
Author(s):  
Iztok Dogsa ◽  
Kumari Sonal Choudhary ◽  
Ziva Marsetic ◽  
Sanjarbek Hudaiberdiev ◽  
Roberto Vera ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Quorum Sensing ◽  
Sensing Systems ◽  
Prokaryotic Genomes
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