scholarly journals The Rocky Road From Fed-Batch to Continuous Processing With E. coli

2019 ◽  
Vol 7 ◽  
Author(s):  
Julian Kopp ◽  
Christoph Slouka ◽  
Oliver Spadiut ◽  
Christoph Herwig
Keyword(s):  
Continuous Processing ◽  
Fed Batch ◽  
E Coli

scholarly journals Different induction regimes in recombinant E. coli fed batch cultivations to modulate inclusion body formation, disassembly or prevention

2009 ◽  
Vol 25 ◽  
pp. S213
Author(s):  
M. Cserjan-Puschmann ◽  
M. Luchner ◽  
F. Clementschitsch ◽  
G. Striedner ◽  
K. Bayer
Keyword(s):  
Inclusion Body ◽  
Fed Batch ◽  
Body Formation ◽  
E Coli ◽  
Inclusion Body Formation

scholarly journals Differential expression of small RNAs under chemical stress and fed-batch fermentation in E. coli

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Martin Holm Rau ◽  
Klara Bojanovič ◽  
Alex Toftgaard Nielsen ◽  
Katherine S. Long
Keyword(s):  
Differential Expression ◽  
Small Rnas ◽  
Batch Fermentation ◽  
Chemical Stress ◽  
Fed Batch ◽  
E Coli ◽  
Fed Batch Fermentation

Co-Expression of Threonine Dehydratase and Acetolactate Synthase in Escherichia Coli for L-Isoleucine Production

2014 ◽  
Vol 989-994 ◽  
pp. 997-1002 ◽  
Author(s):  
Jian Wang ◽  
Jia Kai Sun ◽  
Qing Yang Xu
Keyword(s):  
Escherichia Coli ◽  
Corynebacterium Glutamicum ◽  
Carbon Flux ◽  
Batch Fermentation ◽  
Acetolactate Synthase ◽  
Fed Batch ◽  
Threonine Dehydratase ◽  
E Coli ◽  
Fed Batch Fermentation ◽  
Fermentation Period

Metabolic engineering ofCorynebacterium glutamicumhas sought to divert carbon into L-isoleucine. However, the fermentation period of this strain is long. TheC.glutamicumYILW strain (LeuL, AHVr, SGr, Leu-MEr) was previously derived by repeated compound mutagenesis which could accumulate 20.2 g/L L-isoleucine in a 5-L jar fermentor. Overexpression of the threonine dehydratase gene (ilvA) fromCorynebacterium glutamicumYILW and coexpression of threonine dehydratase and acetolactate synthase (ilvBN) from it were employed to divert carbon flux toward L-isoleucine. The strainE. coliTRFC with the expression ofilvA could accumulate L-isoleucine of 6.8 g/L without accumulation of any L-threonine by fed-batch fermentation in a 5-L jar fermentor. However, the production of L-isoleucine by the strainE.coliTRFC with the co-expression ofilvA andilvBN was decreased by 19.1%, and the production of L-valine was increased by 40% compared with that ofE. coliTRFC with the expression ofilvA.

From laboratory to pilot plant E. coli fed-batch cultures: optimizing the cellular environment for protein maximization

2013 ◽  
Vol 40 (3-4) ◽  
pp. 335-343 ◽  
Author(s):  
J. Ruiz ◽  
A. Fernández-Castané ◽  
C. de Mas ◽  
G. González ◽  
J. López-Santín
Keyword(s):  
Pilot Plant ◽  
Fed Batch ◽  
Batch Cultures ◽  
E Coli ◽  
Cellular Environment

scholarly journals Modelling concentration gradients in fed-batch cultivations of E. coli  - towards the flexible design of scale-down experiments

2018 ◽  
Vol 94 (2) ◽  
pp. 516-526 ◽  
Author(s):  
Emmanuel Anane ◽  
Annina Sawatzki ◽  
Peter Neubauer ◽  
Mariano Nicolas Cruz-Bournazou
Keyword(s):  
Flexible Design ◽  
Fed Batch ◽  
Concentration Gradients ◽  
E Coli ◽  
Scale Down
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