scholarly journals Use of Non-Conventional Cell Disruption Method for Extraction of Proteins from Black Yeasts

2016 ◽  
Vol 4 ◽  
Author(s):  
Maja Čolnik ◽  
Mateja Primožič ◽  
Željko Knez ◽  
Maja Leitgeb
Keyword(s):  
Cell Disruption ◽  
Black Yeasts ◽  
Conventional Cell ◽  
Disruption Method

Evaluation of cell disruption method for lipase extraction from novel thraustochytrids

2017 ◽  
Vol 25 ◽  
pp. 62-67 ◽  
Author(s):  
Avinesh R. Byreddy ◽  
Nalam Madhusudhana Rao ◽  
Colin J. Barrow ◽  
Munish Puri
Keyword(s):  
Cell Disruption ◽  
Disruption Method

scholarly journals Current Pretreatment/Cell Disruption and Extraction Methods Used to Improve Intracellular Lipid Recovery from Oleaginous Yeasts

2021 ◽  
Vol 9 (2) ◽  
pp. 251
Author(s):  
Muhammad Fakhri Zainuddin ◽  
Chong Kar Fai ◽  
Arbakariya B. Ariff ◽  
Leonardo Rios-Solis ◽  
Murni Halim
Keyword(s):  
Solvent Extraction ◽  
Osmotic Shock ◽  
Extraction Methods ◽  
Resistant Cell ◽  
Cell Disruption ◽  
Systematic Evaluation ◽  
Green Solvents ◽  
Oleaginous Yeasts ◽  
Disruption Method ◽  
Recovery Methods

The production of lipids from oleaginous yeasts involves several stages starting from cultivation and lipid accumulation, biomass harvesting and finally lipids extraction. However, the complex and relatively resistant cell wall of yeasts limits the full recovery of intracellular lipids and usually solvent extraction is not sufficient to effectively extract the lipid bodies. A pretreatment or cell disruption method is hence a prerequisite prior to solvent extraction. In general, there are no recovery methods that are equally efficient for different species of oleaginous yeasts. Each method adopts different mechanisms to disrupt cells and extract the lipids, thus a systematic evaluation is essential before choosing a particular method. In this review, mechanical (bead mill, ultrasonication, homogenization and microwave) and nonmechanical (enzyme, acid, base digestions and osmotic shock) methods that are currently used for the disruption or permeabilization of oleaginous yeasts are discussed based on their principle, application and feasibility, including their effects on the lipid yield. The attempts of using conventional and “green” solvents to selectively extract lipids are compared. Other emerging methods such as automated pressurized liquid extraction, supercritical fluid extraction and simultaneous in situ lipid recovery using capturing agents are also reviewed to facilitate the choice of more effective lipid recovery methods.

scholarly journals THE DETERMINATION OF SALINITY AND NUTRITION (NaH2PO4) PROFILE IN Nannochloropsis oculata CULTIVATION TO GAIN MAXIMUM LIPID

REAKTOR ◽  
2012 ◽  
Vol 14 (2) ◽  
pp. 135
Author(s):  
Elida Purba ◽  
Kenjiro Siburian
Keyword(s):  
Total Lipid ◽  
Lipid Content ◽  
Nannochloropsis Oculata ◽  
Cell Disruption ◽  
Disruption Method

The determination of salinity and nutrition (NaH2PO4) profile in Nannochloropsis oculata cultivationto gain maximum lipid was investigated. The purpose of this research was to determine the optimumsalinity and nutrition (NaH2PO4) to gain maximum lipid in Nannochloropsis oculata. Phosphor canbe obtained by adding NaH2PO4 and a salinity enhancement can be applied by adding NaCl to theculture. This research was run by matching each variable of salinity (33, 34, 35, and 36 ppt) to eachvariable of NaH2PO4 (5, 10 and 15 ppm) randomly. The results show that the percentage of maximumlipid in Nannochloropsis oculata was obtained at 35 ppt and 5 ppm NaH2PO4 (37.68 %). Neverthelessthe maximum lipid percentage is low and it can be caused by some factors, such as the dryingtemperature for preparing the dry microalgae to extract, the cell disruption method and the extractionsolvent used. By seeing the results, salinity can affect the total lipid in Nannochloropsis oculata. Thegreater the salinity in culture the greater the lipid content in Nannochloropsis oculata. Otherwise adifferent response was obtained by adding NaH2PO4. If NaH2PO4 concentration is greater, the lipidcontained Nannochloropsis oculata will be lower Penentuan profilsalinitas dan nutrisi (NaH2PO4) pada pembiakan Nannochloropsis oculata untuk memperoleh lipidmaksimum diteliti. Tujuan penelitian ini adalah untuk menentukan salinitas dan nutrisi (NaH2PO4)optimum untuk memperoleh lipid maksimum pada Nannochloropsis oculata. Fosfor dapat diperolehdengan menambahkan NaH2PO4 pada kultur dan peningkatan salinitas dapat dilakukan denganmenambahkan NaCl pada kultur. Penelitian ini dijalankan dengan mencocokkan setiap variabelsalinitas (33, 34, 35, dan 36 ppt) dengan setiap variabel NaH2PO4 (5, 10 dan 15 ppm) secara acak.Hasil menunjukkan bahwa persentase lipid maksimum pada Nannochloropsis oculata diperoleh padasalinitas 35 ppt dan 5 ppm NaH2PO4 (37,68 %). Walaupun demikian, persentase lipid maksimummasih tergolong rendah dan hal ini dapat disebabkan oleh beberapa faktor, seperti temperaturpengeringan yang diterapkan, metode cell disruption dan pelarut ekstraksi yang digunakan. Denganmempelajari hasil yang telah didapat, salinitas dapat mempengaruhi lipid total padaNannochloropsis oculata. Semakin tinggi salinitas pada biakan, semakin tinggi kandungan lipid padaNannochloropsis oculata. Sebaliknya didapatkan respon yang berbeda saat menambahkan NaH2PO4.Apabila konsentrasi NaH2PO4 semakin tinggi, maka lipid yang terkandung pada Nannochloropsisoculata akan menjadi lebih rendah.

In search of an effective cell disruption method to isolate intact mitochondria from Chinese hamster ovary cells

2013 ◽  
Vol 14 (2) ◽  
pp. 161-169 ◽  
Author(s):  
Janina Bahnemann ◽  
Sabrina Kayo ◽  
Judith Wahrheit ◽  
Elmar Heinzle ◽  
Ralf Pörtner ◽  
...  
Keyword(s):  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Cell Disruption ◽  
Ovary Cells ◽  
Disruption Method

Effect of sludge cell disruption on compactibility of biological sludges

2000 ◽  
Vol 42 (9) ◽  
pp. 119-126 ◽  
Author(s):  
A. Erdincler ◽  
P. A. Vesilind
Keyword(s):  
Water Distribution ◽  
Interstitial Water ◽  
Alkali Treatment ◽  
Cell Structure ◽  
Solid Content ◽  
Cell Disruption ◽  
Water Release ◽  
Water Binding ◽  
Biological Sludge ◽  
Disruption Method

Biological sludge contains various fractions of water associated with sludge solids which are mostly microorganisms. These water fractions affect the liquid-solid separation of sludge. A considerable amount of sludge water is trapped either inside the sludge microorganisms or within the floc structure and this is labeled interstitial water. Release of interstitial water held inside the cell structure involves disruption of sludge cells and this does not occur during conventional dewatering. In this study, sludge cell disruption is introduced as a new method to improve the compactibility of sludge. Biological sludge cells are disrupted by different methods including alkali treatment, NaCl treatment, heat treatment, and sonication. The effect of cell disruption on compactibility of biological sludge is investigated. The results of the study indicate that the disruption of the sludge cells changes the water distribution in sludge and improves the compactibility of sludge. Disruption apparently releases from 60% to 80% of interstitial water, depending on the disruption method used. On the other hand, it causes creation of extra surfaces for water binding and leads to an increase in the unfreezable water content (vicinal water, water of hydration and a fraction of interstitial water) of sludge. The cell disruption increases the solid content of compacted sludge up to 87% depending on the cell disruption method used.

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