scholarly journals Effect of heat stress on mitogen-activated protein kinases in the hypothalamic–pituitary–gonadal axis of developing Wenchang chicks

2020 ◽  
Vol 99 (1) ◽  
pp. 567-577
Author(s):  
Q.H. Li ◽  
Z.Q. Yu ◽  
Z. Chen
Keyword(s):  
Heat Stress ◽  
Protein Kinases ◽  
Mitogen Activated Protein Kinases ◽  
Mitogen Activated Protein

scholarly journals The effect of chronic doxorubicin treatment on mitogen-activated protein kinases and heat stress proteins in rat hearts

2008 ◽  
pp. S97-S102
Author(s):  
P Šimončíková ◽  
T Ravingerová ◽  
M Barančík
Keyword(s):  
Heat Stress ◽  
Protein Kinases ◽  
Stress Proteins ◽  
Contractile Function ◽  
Ischemia Reperfusion ◽  
Tissue Samples ◽  
Mitogen Activated Protein Kinases ◽  
Rat Hearts ◽  
Mitogen Activated Protein ◽  
Heat Stress Proteins

The study has been designed to characterize protein systems involved in the responses of rat hearts to chronic doxorubicin (DOX) treatment. We investigated the influence of DOX on cardiac function, mitogen-activated protein kinases (MAPKs) and heat stress proteins (HSPs). Doxorubicin was administered to rats by intraperitoneal injections over a period of 6 weeks. In control and DOX-treated hearts exposed to 20 min global ischemia and 40 min reperfusion the recovery of contractile function after ischemia/reperfusion (I/R) was determined. The levels and phosphorylation state of proteins in tissue samples were analyzed using specific antibodies. We found an activation of extracellular signal-regulated kinases (ERKs) in rat hearts exposed to DOX treatment and better recovery of contractile function after I/R. Analysis of HSPs showed that DOX induced up-regulation of the levels of HSP60 and down-regulation of HSP70 levels. The levels and/or specific phosphorylation of other studied proteins (p38-MAPK, HSP27, HSP90) were not influenced by DOX. The results point to the possible role of ERKs and some HSPs in mechanisms underlying the response of rat hearts to chronic DOX treatment.

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