scholarly journals Rapid detection of Salmonella in poultry environmental samples using real-time PCR coupled with immunomagnetic separation and whole genome amplification

2019 ◽  
Vol 98 (12) ◽  
pp. 6973-6979 ◽  
Author(s):  
Ji-Yeon Hyeon ◽  
David A. Mann ◽  
Jinquan Wang ◽  
Woo Kyun Kim ◽  
Xiangyu Deng
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Environmental Samples ◽  
Whole Genome Amplification ◽  
Immunomagnetic Separation ◽  
Whole Genome ◽  
Genome Amplification

Improved DOP-PCR???Based Representational Whole-Genome Amplification Using Quantitative Real-Time PCR

2006 ◽  
Vol 15 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Lili??na Z. Feh??r ◽  
Margit Bal??zs ◽  
J??nos Z. Kelemen ◽  
??gnes Zvara ◽  
Istv??n N??meth ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Genome Amplification ◽  
Whole Genome ◽  
Quantitative Real Time Pcr ◽  
Genome Amplification

scholarly journals Whole genome amplification and real-time PCR in forensic casework

BMC Genomics ◽  
2009 ◽  
Vol 10 (1) ◽  
pp. 159 ◽  
Author(s):  
Emiliano Giardina ◽  
Ilenia Pietrangeli ◽  
Claudia Martone ◽  
Stefania Zampatti ◽  
Patrizio Marsala ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Whole Genome Amplification ◽  
Whole Genome ◽  
Genome Amplification ◽  
Forensic Casework

scholarly journals Quantifying genome DNA during whole-genome amplification via quantitative real-time multiple displacement amplification

RSC Advances ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 4617-4621
Author(s):  
Jing Tu ◽  
Yi Qiao ◽  
Yuhan Luo ◽  
Naiyun Long ◽  
Zuhong Lu
Keyword(s):  
Real Time ◽  
Whole Genome Amplification ◽  
Multiple Displacement Amplification ◽  
Whole Genome ◽  
Genome Amplification

Monitoring multiple displacement amplification by fluorescence signals.

scholarly journals Development of a Multiplex Real-Time PCR Assay for Rapid Detection of Tigecycline Resistance Gene tet(X) Variants from Bacterial, Fecal, and Environmental Samples

2020 ◽  
Vol 64 (4) ◽  
Author(s):  
Yulin Fu ◽  
Dejun Liu ◽  
Huangwei Song ◽  
Zhihai Liu ◽  
Haiyang Jiang ◽  
...  
Keyword(s):  
Detection Limit ◽  
Real Time ◽  
Resistance Gene ◽  
Resistance Genes ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Environmental Samples ◽  
Target Gene ◽  
Pcr Assay ◽  
High Level

ABSTRACT We developed a multiplex real-time SYBR green-based PCR assay for rapid detection of tet(X) and its variants, including tet(X1) and tet(X2) and high-level tigecycline resistance genes tet(X3), tet(X4), and tet(X5). We showed that the real-time PCR assay developed had high linearity (R2 ≥ 0.996), sensitivity (low detection limit), and specificity (only the target gene could be amplified significantly) and further evaluated it using bacterial, fecal, and environmental samples.

scholarly journals Quantification of Trace-Level DNA by Real-Time Whole Genome Amplification

PLoS ONE ◽  
2011 ◽  
Vol 6 (12) ◽  
pp. e28661 ◽  
Author(s):  
Min-Jung Kang ◽  
Hannah Yu ◽  
Sook-Kyung Kim ◽  
Sang-Ryoul Park ◽  
Inchul Yang
Keyword(s):  
Real Time ◽  
Whole Genome Amplification ◽  
Whole Genome ◽  
Trace Level ◽  
Genome Amplification

Rapid Detection Method for Bacillus anthracis Using a Combination of Multiplexed Real-Time PCR and Pyrosequencing and Its Application for Food Biodefense

2015 ◽  
Vol 78 (2) ◽  
pp. 355-361 ◽  
Author(s):  
TIMOTHY W. JANZEN ◽  
MATTHEW C. THOMAS ◽  
NORIKO GOJI ◽  
MICHAEL J. SHIELDS ◽  
KRISTEN R. HAHN ◽  
...  
Keyword(s):  
Bacillus Anthracis ◽  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Detection Method ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Health Concern ◽  
Sequential Method ◽  
Critical Gap

Bacillus anthracis, the causative agent of anthrax, has the capacity to form highly resilient spores as part of its life cycle. The potential for the dissemination of these spores using food as a vehicle is a huge public health concern and, hence, requires the development of a foodborne bioterrorism response approach. In this work, we address a critical gap in food biodefense by presenting a novel, combined, sequential method involving the use of real-time PCR and pyrosequencing for the rapid, specific detection of B. anthracis spores in three food matrices: milk, apple juice, and bottled water. The food samples were experimentally inoculated with 40 CFU ml−1, and DNA was extracted from the spores and analyzed after immunomagnetic separation. Applying the combination of multiplex real-time PCR and pyrosequencing, we successfully detected the presence of targets on both of the virulence plasmids and the chromosome. The results showed that DNA amplicons generated from a five-target multiplexed real-time PCR detection using biotin-labeled primers can be used for single-plex pyrosequencing detection. The combined use of multiplexed real-time PCR and pyrosequencing is a novel, rapid detection method for B. anthracis from food and provides a tool for accurate, quantitative identification with potential biodefense applications.

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