scholarly journals Genetic characterization of high-level aminoglycoside-resistant Enterococcus faecalis and Enterococcus faecium isolated from retail chicken meat

2019 ◽  
Vol 98 (11) ◽  
pp. 5981-5988
Author(s):  
Yeong Bin Kim ◽  
Kwang Won Seo ◽  
Se Hyun Son ◽  
Eun Bi Noh ◽  
Young Ju Lee
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Genetic Characterization ◽  
Chicken Meat ◽  
High Level ◽  
Retail Chicken Meat

Characteristics of High-Level Ciprofloxacin-Resistant Enterococcus faecalis and Enterococcus faecium from Retail Chicken Meat in Korea

2018 ◽  
Vol 81 (8) ◽  
pp. 1357-1363 ◽  
Author(s):  
YEONG BIN KIM ◽  
HYUN JOO SEO ◽  
KWANG WON SEO ◽  
HYE YOUNG JEON ◽  
DONG KYU KIM ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Antimicrobial Agents ◽  
Health Hazard ◽  
Point Mutations ◽  
Chicken Meat ◽  
Enterococcal Infection ◽  
Meat Samples ◽  
High Level ◽  
Retail Chicken Meat

ABSTRACTGenes encoding ciprofloxacin resistance in enterococci in animals may be transferred to bacteria in the animal gut and to zoonotic bacteria where they could pose a human health hazard. The objective of this study was to characterize antimicrobial resistance in high-level ciprofloxacin-resistant (HLCR) Enterococcus faecalis and Enterococcus faecium isolated from retail chicken meat. A total of 345 enterococci (335 E. faecalis and 10 E. faecium) were isolated from 200 chicken meat samples. Of these, 85 E. faecalis isolates and 1 E. faecium isolate were confirmed as HLCR enterococci. All 86 HLCR enterococci displayed gyrA-parC point mutations consisting of S83I-S80I (94.2%, 81 isolates), S83F-S80I (2.3%, 2 isolates), S83Y-S80I (2.3%, 2 isolates), and S83Y-S80F (1.2%, 1 isolate). Sixty-one (72.9%) of the 86 HLCR enterococci showed multidrug resistance to three to six classes of antimicrobial agents. Multilocus sequence typing revealed that E. faecalis had 17 different sequence types (ST) and E. faecium had 1 different ST, with ST256 observed most often (44 isolates, 51.8%). Although these results cannot exclude the possibility that pathotypes of enterococci isolated from chicken might represent transmission to or from humans, the foodborne HLCR E. faecalis indicated that the food chain is a potential route of enterococcal infection in humans.

Identification of new sequence types among Enterococcus faecium and Enterococcus faecalis carrying the vanA gene in retail chicken meat

2016 ◽  
Vol 4 ◽  
pp. 72-73 ◽  
Author(s):  
Andrey G. Sacramento ◽  
Rosemeire C. Zanella ◽  
Lara M. de Almeida ◽  
Carlos Pires ◽  
Cecília Popazoglo ◽  
...  
Keyword(s):  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Chicken Meat ◽  
Vana Gene ◽  
Retail Chicken Meat ◽  
Sequence Types

Genetic characterization of high-level gentamicin-resistant strains of Enterococcus faecalis in Iran

2004 ◽  
Vol 50 (10) ◽  
pp. 869-872 ◽  
Author(s):  
Mohammad Mehdi Feizabadi ◽  
Sorour Asadi ◽  
Maryam Zohari ◽  
Sara Gharavi ◽  
Gelavizh Etemadi
Keyword(s):  
Genetic Variation ◽  
Enterococcus Faecalis ◽  
Genetic Characterization ◽  
Enzyme Electrophoresis ◽  
Multilocus Enzyme Electrophoresis ◽  
Gentamicin Resistance ◽  
Resistant Strains ◽  
Plasmid Profiling ◽  
High Level

The prevalence of resistance to high levels of gentamicin among 182 isolates of Enterococcus faecalis from 2 Iranian hospitals was 42%. Gentamicin resistance was associated with conjugative plasmids (>70 kb) in most strains. Fingerprinting using EcoRI and HindIII showed genetic variation among these plasmids and gave evidence of nosocomial outbreaks and persistence of infection in different wards of the study hospitals, as well as transfer of plasmids between genetically diverse isolates. Using EcoRI, hospital-based specific plasmid fingerprints were detected for the isolates that had previously proved to be unrelated by multilocus enzyme electrophoresis, suggesting the persistence of related plasmids at each hospital, though minor changes in these related plasmids could be detected with HindIII.Key words: Enterococcus faecalis, HLGR, plasmid profiling, plasmid fingerprinting.

scholarly journals Molecular characterization of antimicrobial-resistant Enterococcus faecalis and Enterococcus faecium isolated from layer parent stock

2019 ◽  
Vol 98 (11) ◽  
pp. 5892-5899 ◽  
Author(s):  
Yeong Bin Kim ◽  
Kwang Won Seo ◽  
Jong Bo Shim ◽  
Se hyun Son ◽  
Eun Bi Noh ◽  
...  
Keyword(s):  
Molecular Characterization ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Parent Stock

scholarly journals Characterization of Vancomycin-Resistant Enterococcus faecium Isolates from the United States and Their Susceptibility In Vitro to Dalfopristin-Quinupristin

1998 ◽  
Vol 42 (5) ◽  
pp. 1088-1092 ◽  
Author(s):  
G. M. Eliopoulos ◽  
C. B. Wennersten ◽  
H. S. Gold ◽  
T. Schülin ◽  
M. Souli ◽  
...  
Keyword(s):  
United States ◽  
Enterococcus Faecium ◽  
Multiple Drug Resistance ◽  
The United States ◽  
Multiple Drug ◽  
Data Set ◽  
Vancomycin Resistant ◽  
High Level

ABSTRACT In the course of clinical studies with the investigational streptogramin antimicrobial dalfopristin-quinupristin, isolates of vancomycin-resistant Enterococcus faecium were referred to our laboratory from across the United States. Seventy-two percent of the strains were of the VanA type, phenotypically and genotypically, while 28% were of the VanB type. High-level resistance to streptomycin or gentamicin was observed in 86 and 81%, respectively, of the VanA strains but in only 69 and 66%, respectively, of the VanB strains. These enterococci were resistant to ampicillin (MIC for 50% of the isolates tested [MIC50] and MIC90, 128 and 256 μg/ml, respectively) and to the other approved agents tested, with the exception of chloramphenicol (MIC90, 8 μg/ml) and novobiocin (MIC90, 1 μg/ml). Considering all of the isolates submitted, dalfopristin-quinupristin inhibited 86.4% of them at concentrations of ≤1 μg/ml and 95.1% of them at ≤2 μg/ml. However, for the data set comprised of only the first isolate submitted for each patient, 94.3% of the strains were inhibited at concentrations of ≤1 μg/ml and 98.9% were inhibited at concentrations of ≤2 μg/ml. Multiple drug resistance was very common among these isolates of vancomycin-resistant E. faecium, while dalfopristin-quinupristin inhibited the majority at concentrations that are likely to be clinically relevant.

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