scholarly journals Pathophysiological characteristics and gene transcriptional profiling of bone microstructure in a low calcium diet fed laying hens

2019 ◽  
Vol 98 (10) ◽  
pp. 4359-4368 ◽  
Author(s):  
S Jiang ◽  
X.L. Wu ◽  
M.L. Jin ◽  
X.Z. Wang ◽  
Q Tang ◽  
...  
Keyword(s):  
Laying Hens ◽  
Transcriptional Profiling ◽  
Bone Microstructure ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

scholarly journals 318 Total flavonoids from Rhizoma drynariae ameliorate low calcium-induced osteoporosis by regulating the bone metabolism in caged laying hens

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 6-7
Author(s):  
Jie Huang ◽  
Lei Zhang ◽  
Zhongxin Zhou
Keyword(s):  
Bone Density ◽  
Laying Hens ◽  
Test Group ◽  
Economic Losses ◽  
Control Group ◽  
Total Flavonoids ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium ◽  
Rhizoma Drynariae

Abstract Cage layer osteoporosis (CLO) is one of the most serious bone metabolic diseases in laying hens industry. This disease not only causes huge economic losses, but also greatly affects the welfare of animals. Traditional Chinese medicines are widely used for prevention and treatment of all kinds of diseases. It is famous for excellent treatment, low side effects, and wide range of use safety. Total flavonoids from Rhizoma drynariae (TFRD) is an herbal product extracted from the dried root of Rhizoma drynariae. Recently, many researcher reports about the beneficial effects of TFRD in relation to various types of bone conditions but no report is available about promoting effect of TFRD against CLO. Therefore, current study was conducted to explore the ameliorating effect of TFRD in low calcium-induced osteoporosis. A total of 144 Lohmann pink-shell laying hens of 31-week-old were equally distributed in four groups: The control group (containing calcium 3.5% in based diet), the low calcium group (containing calcium 1.5% in low-calcium diet), the test group 1 (adding 500 mg/kg TFRD to low-calcium diet), the test group 2 (adding 2000 mg/kg TFRD to low-calcium diet). The birds were fed the diets for 60 d. The bone mineral density of tibia was measured by dual-energy X-ray absorptiometry indicating that the low calcium group had lower bone density than the control group. Compared with the low calcium group, the supplementation of TFRD protects bone tissue and prevents bone density from decreasing. Serum biochemical analysis showed that TFRD supplementation decreased the level of alkaline phosphatase, tartrate-resistant acid phosphatase, osteocalcin and calcitonin. Therefore, adding TFRD to low-calcium diet can improve the activity of important biochemical markers, clearly inhibit bone resorption and alleviate osteoporosis induce by low-calcium diet in caged laying hens.

scholarly journals Icariin protects against cage layer osteoporosis by intervening in steroid biosynthesis and glycerophospholipid metabolism

2021 ◽  
Vol 1 (1) ◽  
Author(s):  
Zhengwang Yu ◽  
Jie Huang ◽  
Zhongxin Zhou
Keyword(s):  
Metabolic Diseases ◽  
Chinese Herb ◽  
Therapeutic Effects ◽  
Mineral Density ◽  
Steroid Biosynthesis ◽  
Metabolic Pathway Analysis ◽  
Effective Prevention ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

AbstractCage layer osteoporosis (CLO) is a common bone metabolism disease in the breeding industry of China. However, effective prevention for CLO has not been developed. Icariin (ICA), the main bioactive component of the Chinese herb Epimedium, has been shown to have good therapeutic effects on bone-related diseases. In this study, the effects of ICA were further evaluated in a low-calcium diet-induced CLO, and a serum metabolomics assay was performed to understand the underlying mechanisms. A total of 144 31-wk-old Lohmann pink-shell laying hens were randomly allocated to 4 groups with 6 replicates of 6 hens per replicate. The 4 dietary treatment groups consisted of a basal diet (3.5% calcium), a low-calcium diet (2.0% calcium), and a low-calcium diet supplemented with 0.5 or 2.0 g/kg ICA. The results showed that ICA exerted good osteoprotective effects on low-calcium diet-induced CLO. ICA significantly increased femur bone mineral density, improved bone microstructure, decreased bone metabolic level, and upregulated mRNA expression of bone formation genes in femoral bone tissue. Serum untargeted metabolomics analysis showed that 8 metabolite levels were significantly changed after ICA treatment, including increased contents of 7-dehydrocholesterol, 7-oxocholesterol, desmosterol, PC (18:1(9Z)/18:1(9Z)), PS (18:0/18:1(9Z)), N,N-dimethylaniline and 2-hydroxy-butanoic acid and decreased N2,N2-dimethylguanosine. Metabolic pathway analysis based on the above 8 metabolites indicated that ICA mainly perturbed steroid biosynthesis and glycerophospholipid metabolism. These findings suggest that ICA can effectively prevent bone loss in low-calcium diet-induced CLO by mediating steroid biosynthesis and glycerophospholipid metabolism and provide new information for the regulation of bone metabolic diseases.

scholarly journals Intestinal Resistance to 1,25 Dihydroxyvitamin D in Mice Heterozygous for the Vitamin D Receptor Knockout Allele

Endocrinology ◽  
2007 ◽  
Vol 148 (3) ◽  
pp. 1396-1402 ◽  
Author(s):  
Yurong Song ◽  
James C. Fleet
Keyword(s):  
Vitamin D ◽  
Vitamin D Receptor ◽  
Time Course ◽  
Transient Receptor Potential ◽  
Gene Knockout ◽  
Mrna Levels ◽  
Wild Type ◽  
Calcium Diet ◽  
Low Calcium Diet ◽  
Low Calcium

We tested the hypothesis that low vitamin D receptor (VDR) level causes intestinal vitamin D resistance and intestinal calcium (Ca) malabsorption. To do so, we examined vitamin D regulated duodenal Ca absorption and gene expression [transient receptor potential channel, vallinoid subfamily member 6 (TRPV6), 24-hydroxylase, calbindin D9k (CaBP) mRNA, and CaBP protein] in wild-type mice and mice with reduced tissue VDR levels [i.e. heterozygotes for the VDR gene knockout (HT)]. Induction of 24-hydroxylase mRNA levels by 1,25 dihydroxyvitamin D3 [1,25(OH)2 D3] injection was significantly reduced in the duodenum and kidney of HT mice in both time-course and dose-response experiments. TRPV6 and CaBP mRNA levels in duodenum were significantly induced after 1,25(OH)2 D3 injection, but there was no difference in response between wild-type and HT mice. Feeding a low-calcium diet for 1 wk increased plasma PTH, renal 1α-hydroxylase (CYP27B1) mRNA level, and plasma 1,25(OH)2 D3, and this response was greater in HT mice (by 88, 55, and 37% higher, respectively). In contrast, duodenal TRPV6 and CaBP mRNA were not higher in HT mice fed the low-calcium diet. However, the response of duodenal Ca absorption and CaBP protein to increasing 1,25(OH)2 D3 levels was blunted by 40% in HT mice. Our data show that low VDR levels lead to resistance of intestinal Ca absorption to 1,25(OH)2 D3, and this resistance may be due to a role for the VDR (and VDR level) in the translation of CaBP.

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