scholarly journals Expression of digestive enzyme and intestinal transporter genes during chronic heat stress in the thermally manipulated broiler chicken

2019 ◽  
Vol 98 (9) ◽  
pp. 4113-4122 ◽  
Author(s):  
Mohammad Borhan Al-Zghoul ◽  
Aseel Ra’ed Said Alliftawi ◽  
Khaled Musa Mohammad Saleh ◽  
Ziad Waheed Jaradat
Keyword(s):  
Heat Stress ◽  
Broiler Chicken ◽  
Digestive Enzyme ◽  
Intestinal Transporter

scholarly journals The effects of protein, amino acid, and dietary electrolyte balance on broiler chicken performance and blood parameters under heat stress

2016 ◽  
Vol 38 (3) ◽  
pp. 285 ◽  
Author(s):  
Ali Asghar Saki ◽  
Mostafa Maleckey ◽  
Rahele Johari ◽  
Sara Mirzaie Goudarzi ◽  
Mansoureh Abdolmaleki
Keyword(s):  
Heat Stress ◽  
Amino Acid ◽  
Broiler Chicken ◽  
Blood Parameters ◽  
Electrolyte Balance ◽  
Protein Amino Acid

scholarly journals Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken

2008 ◽  
Vol 65 (5) ◽  
pp. 453-458 ◽  
Author(s):  
Carolina de Castro Santos ◽  
Eduardo Francisquine Delgado ◽  
José Fernando Machado Menten ◽  
Aparecida Carla de Moura Pedreira ◽  
Carmen Josefina Contreras Castillo ◽  
...  
Keyword(s):  
Heat Stress ◽  
Broiler Chicken ◽  
Myofibrillar Protein ◽  
Environmental Condition ◽  
Fragmentation Index ◽  
Acute Heat Stress ◽  
Sds Page ◽  
And Migration ◽  
Water Holding

Acute heat stress (AHS) modifies the structure of myofibrils affecting functional properties of meat, mainly the water holding capacity. This experiment aimed to identify changes in proteolysis and migration between the myofibrillar and sarcoplasmatic fractions due to pre-slaughter AHS. Myofibrillar fragmentation index (MFI), SDS-PAGE, western blot of vinculin (WB) and shear force (SF) were determined. Six hundred broilers (Gallus gallus) were slaughtered in three different days (ST). In each ST, groups of ten animals were placed in transport crates and submitted to AHS (35ºC, 75 - 85% RH) for 2 hours. Simultaneously, the non-stressed broilers (NS) were kept in thermoneutral environment (22ºC, 83 ± 6.6% RH) within the crates in the same density. After slaughter, the breast muscles were kept refrigerated until the withdrawal of all samples (0, 1, 2, 6 and 24 hours after slaughter). Sampling within AHS and NS birds was collected according to lightness value (normal L* < 49, and high > 51), except for determination of MFI and SF. The lightness was used later to perform SDS-PAGE and WB analyses. MFI kinetics showed that the fragmentation rate was superior in animals NS, indicating that AHS can harm proteolysis and rate of myofibrillar fragmentation. However, the extent of fragmentation did not change, as well as SF values. SDS-PAGE for Troponin fragments indicated a differentiated pattern between AHS and NS. The WB did not show alterations in vinculin fragmentation. Modifications in sarcoplasmatic fraction are observed in meat with high L*values, independent of environmental condition.

scholarly journals Effect of Heat Stress on Body Weight Gain, Heterophile-Lymphocite Ratio and Body Temperature in Broiler

2016 ◽  
Vol 3 (1) ◽  
Author(s):  
Sugito S ◽  
Mira Delima
Keyword(s):  
Body Weight ◽  
Heat Stress ◽  
Weight Gain ◽  
Body Temperature ◽  
Broiler Chicken ◽  
Body Weight Gain ◽  
Conversion Ratio ◽  
Feed Conversion Ratio ◽  
Feed Conversion ◽  
Reduced Body

Increasing in ambient temperature inside the cage could lead to heat stress in broilers. This research was conducted to find out effects of heat stress on body weight gain, heterophile-lymphocite ratio and body temperature in chicken broiler. Twenty broilers aged 20 days (strain Cobb) were randomly divided into 2 groups. The first group was treated with no heat stress, the second one was caged in 33±1 0C temperature for 4 hours per day for 14 days. The results indicated that heat stress reduced body weight gain, increased body temperature, and changed behavior, but no effect on feed conversion ratio (FCR) and heterophile-lymphocyte ratio. It suggested that the heat stress caused detrimental effects on broiler chicken.

scholarly journals Probiotic feeding effect of Bacillus subtilis on broilers chicks’ microflora, TLRs and interleukin gene expression

2021 ◽  
Author(s):  
Zaigui Wang ◽  
Ru Yang ◽  
Salman Khan ◽  
Chunjie wei ◽  
Hu Qian ◽  
...  
Keyword(s):  
Enzyme Activity ◽  
Bacillus Subtilis ◽  
Protease Activity ◽  
Broiler Chicken ◽  
Amylase Activity ◽  
Digestive Enzyme ◽  
Digestive Enzyme Activity ◽  
Control Group ◽  
Broiler Chicks ◽  
Treatment Groups

Abstract The broiler chicks provide the major portion of daily food items. The current study aimed to investigate the effects of Bacillus subtilis (BS) on broiler chicken gut microflora diversity, digestive enzyme activity and expression of Toll-like receptors (TLRs). A total of 240 crossbred broiler chickens were randomly allocated into 4 groups with 3 replicates. The groups were named as control group (basal diet, BD), group Ⅰ (BD with 300 g/d BS,1.08×107 CFU/kg), group Ⅱ (BD with 600 g/d BS, 2.16×107 CFU/kg), and group Ⅲ (BD with 900 g/d BS, 3.24×107 CFU/kg). Samples were collected at the 21st day. The difference of α diversity and β diversity between control group and treatment groups were not significant, but the abundance of some microorganisms in the treatment groups were improved, and the core microorganisms were different between groups. In comparison of control group, the protease activity in ileum of experimental groups was significantly increased (p < 0.05). Protease activity in ileum of group Ⅱ and Ⅲ was improved by 22.59% (p < 0.01) and 14.49% (p < 0.05). The amylase activity in ileum of treatment groups were also significantly increased (p < 0.05). Amylase activity of group Ⅱ was increased by 41.85% (p < 0.01) in comparison to control, group Ⅰ and Ⅲ. Expression of TLR1A and TLR7 in the jejunum and caecum of treatment groups were significantly up-regulated while their expression in ileum was decreased. Similarly, the expression of TLR1B gene was significantly improved in the ileum, and down regulated in the cecum. Transcription levels of TLR2A and MyD88 in jejunum, liver, spleen and kidney were significantly increased, but their expression was significantly lower in the ileum and cecum. The TLR2B, TLR3, TLR4, TLR15, TLR21 expression were also significantly modulated in various organ of broiler chicken. The most important inflammatory factor such as IL-1β expressions in spleen and kidney were significantly increased in the current data. Bacillus subtilis could not regulate the caecal microflora diversity, but improved amount of some gut probiotics such as lactobacillus, the digestive enzyme activity, regulate some immunogenic expressions and enhance the immune capacity of animal. We hope that the impact of this data will explore the diseases control and feeding quality of broiler chicks at industrial level.

Effect of Protein Synthesis Modulator and Acute Heat Stress on Jejunal Morphology in Broiler Chicken

2016 ◽  
Vol 7 (6) ◽  
pp. 1361-1364
Author(s):  
S. K. Jaiswal ◽  
◽  
J. S. Tyagi ◽  
G. Kolluri ◽  
M. Gopi ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Heat Stress ◽  
Broiler Chicken ◽  
Acute Heat Stress
Sign in / Sign up

Export Citation Format

Share Document