scholarly journals McbR is involved in biofilm formation and H2 O2 stress response in avian pathogenic Escherichia coli X40

2019 ◽  
Vol 98 (9) ◽  
pp. 4094-4103 ◽  
Author(s):  
Lumin Yu ◽  
Wenchang Li ◽  
Kezong Qi ◽  
Siyu Wang ◽  
Xiaolin Chen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Stress Response ◽  
Biofilm Formation ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

Biofilm Formation by Avian Pathogenic Escherichia coli is Not Related to In Vivo Pathogenicity

2018 ◽  
Vol 76 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Suelle V. Rodrigues ◽  
Vanessa Laviniki ◽  
Karen A. Borges ◽  
Thales Q. Furian ◽  
Hamilton L. S. Moraes ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

Rutin inhibits quorum sensing, biofilm formation and virulence genes in avian pathogenic Escherichia coli

2018 ◽  
Vol 119 ◽  
pp. 54-59 ◽  
Author(s):  
Lu-Yuan Peng ◽  
Meng Yuan ◽  
Zhen-Qiang Cui ◽  
Zong-Mei Wu ◽  
Zhen-Jiang Yu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Quorum Sensing ◽  
Biofilm Formation ◽  
Virulence Genes ◽  
Avian Pathogenic Escherichia Coli ◽  
Pathogenic Escherichia Coli

scholarly journals Colistin Induces Resistance through Biofilm Formation, via Increased phoQ Expression, in Avian Pathogenic Escherichia coli

Pathogens ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 1525
Author(s):  
Na-Hye Park ◽  
Seung-Jin Lee ◽  
Eon-Bee Lee ◽  
Biruk Tesfaye Birhanu ◽  
Seung-Chun Park
Keyword(s):  
Escherichia Coli ◽  
Biofilm Formation ◽  
Resistant Bacteria ◽  
Avian Pathogenic Escherichia Coli ◽  
Mutant Selection ◽  
Multiple Exposures ◽  
Pathogenic Escherichia Coli ◽  
Mutant Prevention Concentration ◽  
Resistant Strains ◽  
Kill Curve

This study aimed to optimize the colistin-based antibacterial therapy to prevent antimicrobial resistance related to biofilm formation in avian pathogenic Escherichia coli (APEC) in chicken. Of all the bacterial isolates (n = 136), 69 were identified as APEC by polymerase chain reaction (PCR). Through a series of antibiotic susceptibility tests, susceptibility to colistin (<2 μg/mL) was confirmed in all isolates. Hence, a mutant selection window (MSW) was determined to obtain colistin-induced resistant bacteria. The minimum inhibitory concentration (MIC) of colistin against the colistin-induced resistant APEC strains ranged from 8 to 16 μg/mL. To identify the inhibitory activity of colistin against the resistant strains, the mutant prevention concentration (MPC) was investigated for 72 h, and the single and multi-dose colistin activities were determined through the time-kill curve against APEC strains. Bacterial regrowth occurred after 12 h at a double MIC50 concentration (1.00 μg/mL), and regrowth was not inhibited even during multiple exposures. However, upon exposure to 8 μg/mL—a concentration that was close to the MPC—the growth of APEC was inhibited, including in the resistant strains. Additionally, colistin-induced resistant strains showed a slower growth compared with the susceptible ones. Colistin-induced resistant APEC strains did not show colistin resistance gene (mcr-1). However, the expression of higher mgrB and phoQ levels was observed in the resistant strains. Furthermore, these strains showed increased formation of biofilm. Hence, the present study indicated that colistin could induce resistance through the increased formation of biofilm in APEC strains by enhancing the expression of phoQ.

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