scholarly journals Exposure of ciprofloxacin-resistant Escherichia coli broiler isolates to subinhibitory concentrations of a quaternary ammonium compound does not increase antibiotic resistance gene transfer

2019 ◽  
Vol 98 (7) ◽  
pp. 2972-2976 ◽  
Author(s):  
H. Maertens ◽  
K. De Reu ◽  
E. Meyer ◽  
S. Van Weyenberg ◽  
J. Dewulf ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Gene Transfer ◽  
Resistance Gene ◽  
Quaternary Ammonium ◽  
Antibiotic Resistance Gene ◽  
Quaternary Ammonium Compound ◽  
Ammonium Compound ◽  
Subinhibitory Concentrations

scholarly journals Monitoring horizontal antibiotic resistance gene transfer in a colonic fermentation model

2011 ◽  
Vol 78 (2) ◽  
pp. 210-219 ◽  
Author(s):  
Martina C. Haug ◽  
Sabine A. Tanner ◽  
Christophe Lacroix ◽  
Marc J.A. Stevens ◽  
Leo Meile
Keyword(s):  
Antibiotic Resistance ◽  
Gene Transfer ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Colonic Fermentation ◽  
Fermentation Model

scholarly journals Plasmid with Colistin Resistance Genemcr-1in Extended-Spectrum-β-Lactamase-Producing Escherichia coli Strains Isolated from Pig Slurry in Estonia

2016 ◽  
Vol 60 (11) ◽  
pp. 6933-6936 ◽  
Author(s):  
Age Brauer ◽  
Kaidi Telling ◽  
Mailis Laht ◽  
Piret Kalmus ◽  
Irja Lutsar ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Dna Transfer ◽  
The Other ◽  
Pig Slurry ◽  
Colistin Resistance ◽  
Content Type ◽  
Slurry Sample

ABSTRACTA plasmid carrying the colistin resistance genemcr-1was isolated from a pig slurry sample in Estonia. The gene was present on a 33,311-bp plasmid of the IncX4 group.mcr-1is the only antibiotic resistance gene on the plasmid, with the other genes mainly coding for proteins involved in conjugative DNA transfer (taxA,taxB,taxC,trbM, and thepilXoperon). The plasmid pESTMCR was present in three phylogenetically very differentEscherichia colistrains, suggesting that it has high potential for horizontal transfer.

scholarly journals eDNA Inactivation and Biofilm Inhibition by the Polymeric Biocide Polyhexamethylene Guanidine Hydrochloride (PHMG-Cl)

2022 ◽  
Vol 23 (2) ◽  
pp. 731
Author(s):  
Olena V. Moshynets ◽  
Taras P. Baranovskyi ◽  
Olga S. Iungin ◽  
Nadiia P. Kysil ◽  
Larysa O. Metelytsia ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Gene Transfer ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Guanidine Hydrochloride ◽  
Dust Particles ◽  
Biofilm Inhibition ◽  
Water Contaminants ◽  
Polyhexamethylene Guanidine ◽  
Biofilm Development

The choice of effective biocides used for routine hospital practice should consider the role of disinfectants in the maintenance and development of local resistome and how they might affect antibiotic resistance gene transfer within the hospital microbial population. Currently, there is little understanding of how different biocides contribute to eDNA release that may contribute to gene transfer and subsequent environmental retention. Here, we investigated how different biocides affect the release of eDNA from mature biofilms of two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus ATCC 25923 (SA) and contribute to the hospital resistome in the form of surface and water contaminants and dust particles. The effect of four groups of biocides, alcohols, hydrogen peroxide, quaternary ammonium compounds, and the polymeric biocide polyhexamethylene guanidine hydrochloride (PHMG-Cl), was evaluated using PA and SA biofilms. Most biocides, except for PHMG-Cl and 70% ethanol, caused substantial eDNA release, and PHMG-Cl was found to block biofilm development when used at concentrations of 0.5% and 0.1%. This might be associated with the formation of DNA–PHMG-Cl complexes as PHMG-Cl is predicted to bind to AT base pairs by molecular docking assays. PHMG-Cl was found to bind high-molecular DNA and plasmid DNA and continued to inactivate DNA on surfaces even after 4 weeks. PHMG-Cl also effectively inactivated biofilm-associated antibiotic resistance gene eDNA released by a pan-drug-resistant Klebsiella strain, which demonstrates the potential of a polymeric biocide as a new surface-active agent to combat the spread of antibiotic resistance in hospital settings.

scholarly journals eDNA Inactivation and Biofilm Inhibition by the Polymeric Biocide Polyhexamethylene Guanidine Hydrochloride (PHMG-Cl)

2021 ◽  
Author(s):  
Olena V. Moshynets ◽  
Taras P. Baranovskyi ◽  
Olga S. Iungin ◽  
Nadiia P. Kysil ◽  
Larysa O. Metelytsia ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Gene Transfer ◽  
Resistance Gene ◽  
Antibiotic Resistance Gene ◽  
Guanidine Hydrochloride ◽  
Active Agent ◽  
Dust Particles ◽  
Biofilm Inhibition ◽  
Water Contaminants ◽  
Biofilm Development

The choice of effective biocides used for routine hospital practice should consider the role of disinfectants in the maintenance and development of local resistome and how they might affect antibiotic resistance gene transfer within the hospital microbial population. Currently, there is little understanding of how different biocides contribute to eDNA release that may contribute to gene transfer and subsequent environmental retention. Here we investigated how different biocides affected the release of eDNA from mature biofilms of two opportunistic model strains Pseudomonas aeruginosa ATCC 27853 (PA) and Staphylococcus aureus ATCC 25923 (SA) and contribute to the hospital resistome in the form of surface and water contaminants and dust particles. The effect of four groups of biocides including alcohols, hydrogen peroxide, quaternary ammonium compounds, and polymeric guanidines were evaluated using PA and SA biofilms. Most biocides, except for PHMG-Cl and 70% ethanol, caused substantial eDNA release and PHMG-Cl was found to block biofilm development when used at concentrations of 0.5% and 0.1%. This might be associated with the formation of DNA-PHMG-Cl complexes as PHMG-Cl is predicted to bind to AT base pairs by molecular docking assays. PHMG-Cl was found to bind high molecular DNA and plasmid DNA and continued to inactivate DNA on surfaces even after four weeks. PHMG-Cl also effectively inactivated biofilm-associated antibiotic resistance gene eDNA released by a pan-drug-resistant Klebsiella strain which demonstrates the potential of PHMG-Cl as a new surface-active agent to combat the spread of antibiotic resistance in hospital settings.

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