scholarly journals Effect of dietary supplementation of nitrocompounds on Salmonella colonization and ileal immune gene expression in laying hens challenged with Salmonella Enteritidis

2017 ◽  
Vol 96 (12) ◽  
pp. 4280-4286 ◽  
Author(s):  
Pratima Adhikari ◽  
D E Cosby ◽  
N A Cox ◽  
W K Kim
Keyword(s):  
Gene Expression ◽  
Salmonella Enteritidis ◽  
Laying Hens ◽  
Dietary Supplementation ◽  
Immune Gene ◽  
Immune Gene Expression

scholarly journals Effects of a blend of Saccharomyces cerevisiae-based direct-fed microbial and fermentation products in the diet of newly weaned beef steers: growth performance, whole-blood immune gene expression, serum biochemistry, and plasma metabolome1

2019 ◽  
Author(s):  
James A Adeyemi ◽  
David L Harmon ◽  
D M Paulus Compart ◽  
Ibukun M Ogunade
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Dietary Supplementation ◽  
Basal Diet ◽  
Serum Biochemistry ◽  
Immune Gene ◽  
Beef Steers ◽  
Immune Gene Expression ◽  
Plasma Metabolome ◽  
Immune Related Genes

Abstract We examined the effects of dietary supplementation of a Saccharomyces cerevisiae-based direct-fed microbial (DFM) on the growth performance, whole-blood immune gene expression, serum biochemistry, and plasma metabolome of newly weaned beef steers during a 42 d receiving period. Forty newly weaned Angus crossbred steers (7 d post-weaning; 210 ± 12 kg of BW; 180 ± 17 d of age) from a single source were stratified by BW and randomly assigned to 1 of 2 treatments: basal diet with no additive (CON; n = 20) or a basal diet top-dressed with 19 g of the DFM (PROB; n = 20). Daily DMI and weekly body weights were measured to calculate average daily gain (ADG) and feed efficiency (FE). Expression of 84 immune-related genes was analyzed on blood samples collected on days 21 and 42. Serum biochemical parameters and plasma metabolome were analyzed on days 0, 21, and 42. On day 40, fecal grab samples were collected for pH measurement. Compared with CON, dietary supplementation of PROB increased final body weight (P = 0.01) and ADG (1.42 vs. 1.23 kg; P = 0.04) over the 42 d feeding trial. There was a tendency for improved FE with PROB supplementation (P = 0.10). No treatment effect (P = 0.24) on DMI was observed. Supplementation with PROB increased (P ≤ 0.05) the concentrations of serum calcium, total protein, and albumin. Compared with CON, dietary supplementation with PROB increased (P ≤ 0.05) the expression of some immune-related genes involved in detecting pathogen-associated molecular patterns (such as TLR1, TLR2, and TLR6), T-cell differentiation (such as STAT6, ICAM1, RORC, TBX21, and CXCR3) and others such as TNF and CASP1, on day 21 and/or day 42. Conversely, IL-8 was upregulated (P = 0.01) in beef steers fed CON diet on day 21. Plasma untargeted plasma metabolome analysis revealed an increase (P ≤ 0.05) in the concentration of metabolites, 5-methylcytosine and indoleacrylic acid involved in protecting the animals against inflammation in steers fed PROB diet. There was a tendency for lower fecal pH in steers fed PROB diet (P = 0.08), a possible indication of increased hindgut fermentation. This study demonstrated that supplementation of PROB diet improved the performance, nutritional status, and health of newly weaned beef steers during a 42 d receiving period.

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