scholarly journals Growth performance and gastrointestinal responses of broiler chickens fed corn-soybean meal diet without or with exogenous epidermal growth factor upon challenge with Eimeria

2017 ◽  
Vol 96 (10) ◽  
pp. 3676-3686 ◽  
Author(s):  
E. Kim ◽  
H. Leung ◽  
N. Akhtar ◽  
J. Li ◽  
J.R. Barta ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Performance ◽  
Soybean Meal ◽  
Broiler Chickens ◽  
Epidermal Growth ◽  
Exogenous Epidermal Growth Factor

scholarly journals Expression of Gallus Epidermal Growth Factor (gEGF) with Food-Grade Lactococcus lactis Expression System and Its Biological Effects on Broiler Chickens

Biomolecules ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 103
Author(s):  
Yu Zhou ◽  
Pinpin Chen ◽  
Shuai Shi ◽  
Xiaowen Li ◽  
Deshi Shi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Performance ◽  
Lactococcus Lactis ◽  
Broiler Chickens ◽  
Biological Effects ◽  
Expression System ◽  
Control Group ◽  
Fermentation Product ◽  
Epidermal Growth

As a multifunctional polypeptide, epidermal growth factor (EGF) increases growth performance or enhances resistance to diseases in commercial broilers under adverse conditions. In this study, a recombinant Lactococcus lactis was established to produce the secretory form of bioactive gEGF. The results of in vitro testing showed that gEGF promoted the proliferation of chicken embryo fibroblast cells. A total of 63 5-day-old broiler chickens were evenly divided into three groups and treated with either M17 medium (the control group), supernatant of LL-pNZ8149 fermentation product (the P-LL group), or supernatant of LL-pNZ8149-gEGF fermentation product (the gEGF group). In two weeks, many measurements of growth, immunity and the intestines were significantly higher in the gEGF group than those in the control and the P-LL groups. Our study showed that the bioactive gEGF could be expressed with Lactococcus lactis expression system with the potential to enhance growth performance, immune function, and intestinal development in broiler chickens.

scholarly journals Expression of gallus epidermal growth factor (gEGF) with food-grade Lactococcus lactis expression system and its biological effects on broiler chickens

2020 ◽  
Author(s):  
Yu Zhou ◽  
Pinpin Chen ◽  
Shuai Shi ◽  
Xiaowen Li ◽  
Deshi Shi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Performance ◽  
Lactococcus Lactis ◽  
Broiler Chickens ◽  
Biological Effects ◽  
Expression System ◽  
The Other ◽  
Food Grade ◽  
Epidermal Growth

Abstract Background Epidermal growth factor (EGF) is a multifunctional polypeptide, which could be utilized to solve problems in the industry such as increasing growth performance of commercial broilers or enhancing resistance to diseases under adverse conditions. Thus far, very few studies have focused on the gallus epidermal growth factor (gEGF) despite the availability of a plethora of studies on mammalian EGF. The aim of this study was to express gallus epidermal growth factor (gEGF) using a food-grade Lactococcus lactis expression system and to investigate its biological effects on broiler chickens. Results A recombinant Lactococcus lactis, which produced the secretary form of bioactive gEGF at 2.67 μg/mL in culture supernatant, was generated. In vitro testing denoted that gEGF promoted the proliferation of UMNSAH/DF-1 cells. Sixty-three 5-day-old broiler chickens were divided into three groups and treated with either M17 medium (C, as control), supernatant of LL-pNZ8149 fermentation product (P-LL), or supernatant of LL-pNZ8149-gEGF fermentation product (gEGF-P-LL). Body weight (BW), average daily gain (ADG), and the gain:feed ratio of the gEGF-P-LL group were significantly higher than those of the other groups during the two-week study. In addition, two weeks after therapy, the indices of spleen and thymus gland, levels of serum IgA, IgG and duodenum mucosal sIgA were all significantly higher in the gEGF-P-LL group than in the other groups. Moreover, the villus height of the duodenum, jejunum and ileum and crypt depth of the jejunum of the gEGF-P-LL group were significantly higher than those of the other groups, while the crypt depth of the duodenum showed the opposite pattern during the two-week study. Conclusions The bioactive gEGF could be expressed with Lactococcus lactis expression system. Furthermore, gEGF has the potential to enhance growth performance, immune function, and intestinal development in broiler chickens.

scholarly journals Expression of gallus epidermal growth factor (gEGF) with food-grade Lactococcus lactis expression system and its biological effects on broiler chickens

2019 ◽  
Author(s):  
Yu Zhou ◽  
Pinpin Chen ◽  
Shuai Shi ◽  
Xiaowen Li ◽  
Deshi Shi ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Growth Performance ◽  
Lactococcus Lactis ◽  
Broiler Chickens ◽  
Biological Effects ◽  
Expression System ◽  
The Other ◽  
Intestinal Development ◽  
Epidermal Growth

Abstract Background Epidermal growth factor (EGF) is a multifunctional polypeptide, which could be utilized to solve problems in the industry such as increasing growth performance of commercial broilers or enhancing resistance to diseases under adverse conditions. Thus far, very few studies have focused on the gallus epidermal growth factor (gEGF) despite the availability of a plethora of studies on mammalian EGF. The aim of this study was to express gallus epidermal growth factor (gEGF) using a food-grade Lactococcus lactis expression system and to investigate its biological effects on broiler chickens. Results A recombinant Lactococcus lactis producing the secretary form of bioactive gEGF at 2.67 μg/ml in culture supernatant was generated. In vitro testing denoted that gEGF promoted the proliferation of UMNSAH/DF-1 cells. Sixty-three 5-day-old broiler chickens were divided into three groups and treated with either M17 medium (Control), supernatant of LL-pNZ8149 fermentation product (P-LL), or supernatant of LL-pNZ8149-gEGF fermentation product (gEGF-P-LL). Final body weight (FBW), average daily gain (ADG), and the gain:feed ratio of the gEGF-P-LL group were significantly (P<0.05) higher than those of the other groups during the two-week study. In addition, two weeks after therapy, the spleen and thymus indices, levels of serum IgA, IgG, and duodenum mucosal sIgA were all significantly (P<0.05) higher in the gEGF-P-LL group than in the other groups. Moreover, the villus height of the duodenum or jejunum and crypt depth of the jejunum of the gEGF-P-LL group were significantly (P<0.05) higher than those of the other groups, while the crypt depth of the duodenum showed the opposite pattern during the two-week study. Conclusions The bioactive gEGF was expressed with Lactococcus lactis expression system. Furthermore, gEGF has the potential to enhance growth performance, improve immune function, and promote intestinal development in broiler chickens. Keywords: gallus epidermal growth factor, Lactococcus lactis, broiler chickens, growth performance, immune function, intestinal development

Epidermal growth factor accelerates functional recovery from ischaemic acute tubular necrosis in the rat: Role of the epidermal growth factor receptor

1990 ◽  
Vol 78 (5) ◽  
pp. 445-450 ◽  
Author(s):  
Jill Norman ◽  
Yong-Kwei Tsau ◽  
Angelito Bacay ◽  
Leon G. Fine
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Serum Creatinine ◽  
Acute Tubular Necrosis ◽  
Growth Factor Receptor ◽  
Serum Creatinine Concentration ◽  
Creatinine Concentration ◽  
Tubular Necrosis ◽  
Epidermal Growth ◽  
Exogenous Epidermal Growth Factor

1. Severe, ischaemic, acute tubular necrosis was induced in rats by bilateral occlusion of the renal arteries. The experimental group received exogenous epidermal growth factor infused directly into the renal arterial circulation. Serum creatinine concentration was measured daily for 1 week. Epidermal growth factor receptor binding was measured by autoradiography of whole kidney sections. Renal cell proliferation was measured by incorporation of [3H]thymidine into DNA. 2. Serum creatinine concentration increased after acute tubular necrosis with a peak at 48 h and remained elevated above control levels after 7 days. Binding of radiolabelled epidermal growth factor occurred in all regions of the kidney 48 h after ischaemia. Treatment with exogenous epidermal growth factor attenuated the rise in serum creatinine by 4 days after acute tubular necrosis and after 7 days serum creatinine was lower than in animals that did not receive epidermal growth factor. Infusion of epidermal growth factor also increased renal DNA synthesis. 3. The increase in epidermal growth factor binding in the kidney after acute tubular necrosis and the attenuation of the increase in serum creatinine concentration by administration of exogenous epidermal growth factor, suggest a role for epidermal growth factor in recovery from ischaemic damage. The increase in DNA synthesis in response to epidermal growth factor indicates that its effect may be due, at least in part, to accelerated tubular cell proliferation.

The effect of concentration on hepatic transport of exogenous epidermal growth factor

Hepatology ◽  
1985 ◽  
Vol 5 (2) ◽  
pp. 211-214 ◽  
Author(s):  
Susan Jo Burwen ◽  
Mary E. Barker ◽  
Ira S. Goldman ◽  
Albert L. Jones
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Hepatic Transport ◽  
Epidermal Growth ◽  
Exogenous Epidermal Growth Factor
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