scholarly journals Characteristics of glycosaminoglycans in chicken eggshells and the influence of disaccharide composition on eggshell properties

2016 ◽  
Vol 95 (12) ◽  
pp. 2879-2888 ◽  
Author(s):  
Z. Liu ◽  
X. Sun ◽  
C. Cai ◽  
W. He ◽  
F. Zhang ◽  
...  
Keyword(s):  
Disaccharide Composition

scholarly journals Metabolism of rat bone proteoglycans in vivo

1983 ◽  
Vol 216 (3) ◽  
pp. 589-596 ◽  
Author(s):  
C W Prince ◽  
F Rahemtulla ◽  
W T Butler
Keyword(s):  
Extraction Procedure ◽  
Total Radioactivity ◽  
Mineralized Tissue ◽  
Guanidinium Chloride ◽  
Disaccharide Composition ◽  
A Minor ◽  
Papain Digestion ◽  
Rat Bone

Former evaluations of the role of proteoglycans in mineralization have neglected to address the possibility that the metabolism of proteoglycans may be of significance in this regard. This problem was studied by using radiolabeling in vivo of rat calvaria with [35Sulphate for 2-72 h and a sequential extraction procedure to yield two pools of newly synthesized proteoglycans: one obtained from non-mineralized tissue by extraction with guanidinium chloride (GdmCl) and another obtained only after demineralization with EDTA. Total radioactivity in calvaria was maximal after 12 h of incorporation, but by 36 h had declined to a level that was about 55-65% of maximum. Radioactivity in the GdmCl extract declined steadily after 12 h, whereas that in the EDTA extract remained constant until 36 h, when it began to increase. Each extract contained a minor proteoglycan that eluted at the void volume (Vo) of a Sepharose CL-6B column. Unlike in the EDTA extract, this proteoglycan gradually disappeared from the GdmCl extract. Each extract also contained a major, smaller proteoglycan, with a Kav. of 0.24 and 0.36 in the GdmCl and EDTA extracts respectively. Papain digestion of each extract yielded glycosaminoglycan chains with Kav. values of 0.32 and 0.50 on CL-6B in the GdmCl and EDTA extracts respectively. Digestion of each extract with chondroitinase ABC and chondroitinase AC showed that the glycosaminoglycans were of similar disaccharide composition, with about 85% being 4-sulphated and the remainder 6-sulphated and/or iduronic acid-containing. These data suggest that about 45% of the newly synthesized proteoglycans are removed from the tissue during the course of mineralization.

The disaccharide composition of heparins and heparan sulfates

1989 ◽  
Vol 176 (1) ◽  
pp. 96-104 ◽  
Author(s):  
Yuchuan Guo ◽  
H.Edward Conrad
Keyword(s):  
Disaccharide Composition ◽  
Heparan Sulfates

Heparinase Digestion-Based Disaccharide Composition Analysis of Heparin and Heparinoid Drugs

2015 ◽  
Vol 43 (7) ◽  
pp. 964-970 ◽  
Author(s):  
Zhang-Run HAN ◽  
Xin-Hui XING ◽  
Guang-Li YU ◽  
Yang-Yang ZENG ◽  
Li-Juan ZHANG
Keyword(s):  
Composition Analysis ◽  
Disaccharide Composition

Determining the extent of heparan sulfate depolymerisation following heparin lyase treatment

2020 ◽  
Author(s):  
Susan Carnachan ◽  
Tracey Bell ◽  
Ian Sims ◽  
RAA Smith ◽  
V Nurcombe ◽  
...  
Keyword(s):  
Refractive Index ◽  
Heparan Sulfate ◽  
Size Exclusion ◽  
Considerable Proportion ◽  
Bond Scission ◽  
Exclusion Chromatography ◽  
Balance Analysis ◽  
Complete Digestion ◽  
Disaccharide Composition ◽  
Mass Balance Analysis

© 2016 Elsevier Ltd The depolymerisation of porcine mucosal heparan sulfate under the action of heparin lyases and analysis by size-exclusion chromatography (SEC) is described. Heparan sulfate treated to enzymic bond scission producing a Δ4,5 double-bond and quantified by SEC with ultraviolet-visible (UV) spectroscopic detection (230 nm) indicated that the majority of the biopolymer (>85%) was reduced to disaccharides (degree of polymerisation (DP) = 2). However, analysis of the SEC eluant using refractive index (RI), which reflects the mass contribution of the oligosaccharides rather than the molar response of a UV chromophore, indicated that a considerable proportion of the digested HS, up to 43%, was present with DP >2. This was supported by a mass balance analysis. These results contradict the accepted literature where “complete digestion” is routinely reported. Herein we report on the composition and methodology utilised to ascertain the extent of depolymerization and disaccharide composition of this important biopolymer.

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