scholarly journals Mycoplasma gallisepticum transmission: Comparison of commercial F-strain vaccine versus layer complex-derived field strains in a tunnel ventilated house

2012 ◽  
Vol 91 (12) ◽  
pp. 3072-3079 ◽  
Author(s):  
J.L. Purswell ◽  
J.D. Evans ◽  
S.A. Leigh ◽  
S.D. Collier ◽  
H.A. Olanrewaju ◽  
...  
Keyword(s):  
Mycoplasma Gallisepticum ◽  
Strain Vaccine ◽  
Versus Layer

scholarly journals Complete Genome Sequences of Two Mycoplasma gallisepticum F-Strain Variants

2019 ◽  
Vol 8 (33) ◽  
Author(s):  
Spencer A. Leigh ◽  
Jeff D. Evans ◽  
Scott L. Branton
Keyword(s):  
Complete Genome ◽  
Host Response ◽  
Mycoplasma Gallisepticum ◽  
Genome Sequences ◽  
Content Type ◽  
Strain Vaccine

Mycoplasma gallisepticum pathology in poultry is preventable by vaccination with live M. gallisepticum vaccines. Research has suggested possible differences in host response between F-strain-based vaccines. The genomes of the AviPro vaccine and F99 parent strains were sequenced for comparison with the already sequenced F-strain vaccine.

Characterization of pMGA Genes from the F - Strain (Vaccine Strain) of Mycoplasma Gallisepticum

2002 ◽  
Vol 1 (4) ◽  
pp. 63-73
Author(s):  
G. T. Pharr . ◽  
S. L. Branton . ◽  
L. A. Hanson . ◽  
F. C. Minion . ◽  
M. B. Hughlett . ◽  
...  
Keyword(s):  
Vaccine Strain ◽  
Mycoplasma Gallisepticum ◽  
Strain Vaccine

Exciton dynamics in thick GaN MOVPE epilayers deposited on sapphire.

1997 ◽  
Vol 2 ◽  
Author(s):  
J. Allègre ◽  
P. Lefebvre ◽  
J. Camassel ◽  
B. Beaumont ◽  
Pierre Gibart
Keyword(s):  
Layer Thickness ◽  
Buffer Layers ◽  
Rate Equations ◽  
Time Resolved ◽  
Versus Layer ◽  
Level Model ◽  
Shallow Impurities ◽  
Aln Buffer ◽  
Time Resolved Photoluminescence

Time-resolved photoluminescence spectra have been recorded on three GaN epitaxial layers of thickness 2.5 μm, 7 μm and 16 μm, at various temperatures ranging from 8K to 300K. The layers were deposited by MOVPE on (0001) sapphire substrates with standard AlN buffer layers. To achieve good homogeneities, the growth was in-situ monitored by laser reflectometry. All GaN layers showed sharp excitonic peaks in cw PL and three excitonic contributions were seen by reflectivity. The recombination dynamics of excitons depends strongly upon the layer thickness. For the thinnest layer, exponential decays with τ ~ 35 ps have been measured for both XA and XB free excitons. For the thickest layer, the decay becomes biexponential with τ1 ~ 80 ps and τ2 ~ 250 ps. These values are preserved up to room temperature. By solving coupled rate equations in a four-level model, this evolution is interpreted in terms of the reduction of density of both shallow impurities and deep traps, versus layer thickness, roughly following a L−1 law.

scholarly journals GAA Trinucleotide Repeat Region Regulates M9/pMGA Gene Expression in Mycoplasma gallisepticum

2000 ◽  
Vol 68 (2) ◽  
pp. 871-876 ◽  
Author(s):  
Li Liu ◽  
Kevin Dybvig ◽  
Victor S. Panangala ◽  
Vicky L. van Santen ◽  
Christopher T. French
Keyword(s):  
Gene Expression ◽  
Trinucleotide Repeat ◽  
Mycoplasma Gallisepticum ◽  
Avian Host ◽  
Phase Variable ◽  
Repeat Region ◽  
Chromosomal Dna ◽  
Promoter Regions ◽  
Variable Expression ◽  
Gaa Repeats

ABSTRACT Mycoplasma gallisepticum, the cause of chronic respiratory infections in the avian host, possesses a family of M9/pMGA genes encoding an adhesin(s) associated with hemagglutination. Nucleotide sequences of M9/pMGA gene family members indicate extensive sequence similarity in the promoter regions of both the transcribed and silent genes. The mechanism that regulates M9/pMGA gene expression is unknown, but studies have revealed an apparent correlation between gene expression and the number of tandem GAA repeat motifs located upstream of the putative promoter. In this study, transposon Tn4001was used as a vector with the Escherichia coli lacZ gene as the reporter system to examine the role of the GAA repeats in M9/pMGA gene expression in M. gallisepticum. A 336-bp M9 gene fragment (containing the GAA repeat region, the promoter, and the translation start codon) was amplified by PCR, ligated with alacZ gene from E. coli, and inserted into the Tn4001-containing plasmid pISM2062. This construct was transformed into M. gallisepticum PG31. Transformants were filter cloned on agar supplemented with 5-bromo-4-chloro-3-indolyl-β-d-galactopyranoside (X-Gal) to monitor lacZ gene expression on the basis of blue/white color selection. Several cycles of filter cloning resulted in cell lineages in which lacZ gene expression alternated between the On and Off states in successive generations of progeny clones. The promoter regions of the M9-lacZ hybrid genes of individual progeny clones were amplified by PCR and sequenced. The only differences between the promoter regions of the blue and white colonies were in the number of GAA repeats. Clones that expressedlacZ had exactly 12 tandem copies of the GAA repeat. Clones that did not express lacZ invariably had either more than 12 (14 to 16) or fewer than 12 (5 to 11) GAA repeats. Southern analysis of M. gallisepticum chromosomal DNA confirmed that the phase-variable expression of the lacZ reporter gene was not caused by Tn4001 transposition. These data strongly indicate that changes in the length of the GAA repeat region are responsible for regulating M9/pMGA gene expression.

scholarly journals Intratracheal inoculation of human varicella zoster virus (VZV; MAV strain) vaccine successfully induced VZV IgG antibodies in rhesus monkeys

2021 ◽  
Vol 37 (1) ◽  
Author(s):  
Jong-Min Kim ◽  
Chung-Gyu Park
Keyword(s):  
Rhesus Monkey ◽  
Varicella Zoster Virus ◽  
Humoral Immunity ◽  
Antibody Production ◽  
Rhesus Monkeys ◽  
Igg Antibodies ◽  
Simian Varicella Virus ◽  
Varicella Zoster ◽  
Varicella Virus ◽  
Strain Vaccine

Abstract Background The objective of this study was to investigate whether the use of live attenuated varicella zoster virus (VZV) MAV vaccination can efficiently induce VZV antibody production in naive rhesus monkeys as an approach to prevent simian varicella virus (SVV) reactivation in animals immunosuppressed for transplantation studies. Results Clinically available human VZV vaccine was used to induce the production of anti-VZV antibodies in rhesus monkeys. A vial of the vaccine was subcutaneously injected at 0 week, and the second and third vaccination was performed at 5 and 6 weeks by intratracheal inoculation. The titer of anti-VZV IgG was assessed at 0, 2, 4, 6, and 7 weeks. At 2 weeks, 3/16 were seropositive for VZV IgG. At 6 weeks, 9/16 were shown to be seropositive. At 7 weeks, 16/16 were found to be seropositive. Conclusions The VZV vaccine via intratrachael inoculation was shown to induce VZV IgG humoral immunity in rhesus monkeys and may be important immunosuppressed macaques for transplantation studies. Although the humoral immunity produced is an important finding, further studies will be necessary to confirm possible protection and it could protect probably against SVV infection in rhesus monkey.

scholarly journals Phytopathogenicity of avian mycoplasma Mycoplasma gallisepticum S6: Morphologic and ultracytostructural changes in plants infected with the vegetative forms and the viable but nonculturable forms of the bacterium

2010 ◽  
Vol 165 (4) ◽  
pp. 346-350 ◽  
Author(s):  
Vladislav M. Chernov ◽  
Olga A. Chernova ◽  
Alexey A. Mouzykantov ◽  
Anastasia A. Ponomareva ◽  
Maxim V. Trushin ◽  
...  
Keyword(s):  
Mycoplasma Gallisepticum ◽  
Viable But Nonculturable
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