scholarly journals Mitochondrial and nuclear markers for the authentication of partridge meat and the specific identification of red-legged partridge meat products by polymerase chain reaction

2011 ◽  
Vol 90 (1) ◽  
pp. 211-222 ◽  
Author(s):  
M. Rojas ◽  
I. González ◽  
M.Á. Pavón ◽  
N. Pegels ◽  
P.E. Hernández ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Meat Products ◽  
Chain Reaction ◽  
Nuclear Markers ◽  
Specific Identification ◽  
Polymerase Chain ◽  
Mitochondrial And Nuclear Markers

scholarly journals Detection of Salmonella in Meat Products by Polymerase Chain Reaction

2021 ◽  
Vol 41 (1) ◽  
pp. 103-105
Author(s):  
George Armany ◽  
Hemmat Ibrahim ◽  
Reham Amin ◽  
Naglaa Hagag
Keyword(s):  
Polymerase Chain Reaction ◽  
Meat Products ◽  
Chain Reaction ◽  
Polymerase Chain

scholarly journals Collaborative Trial for Validation of a Real-Time Reverse Transcriptase-Polymerase Chain Reaction Assay for Detection of Central Nervous System Tissues as Bovine Spongiform Encephalopathy RiskMaterial: Part 1

2006 ◽  
Vol 89 (5) ◽  
pp. 1335-1340
Author(s):  
Amir Abdulmawjood ◽  
Holger Schnenbrcher ◽  
Michael BÜlte
Keyword(s):  
Central Nervous System ◽  
Polymerase Chain Reaction ◽  
Real Time ◽  
Meat Products ◽  
Spongiform Encephalopathy ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Pcr Assay ◽  
Heat Treated ◽  
Polymerase Chain

Abstract A collaborative trial was conducted to evaluate a real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay for detection of central nervous system (CNS) tissues in meat products (e.g., sausages). The method is based on the detection of ruminant glial fibrillary acidic protein (GFAP) mRNA by applying real-time RT-PCR. The assay was evaluated through a multicenter trial involving 12 participating laboratories that received coded cDNA obtained from 3 different types of sausages. The participants used 5 different real-time detection systems. The results obtained in this validation revealed that this real-time RT-PCR assay performed well in the different laboratories with a detection limit of at least 0.1% CNS in those test materials that contained strongly heat-treated samples (sausages cooked at 120C) and the medium heat-treated samples (sausages cooked at 80C). The detection limit of liver sausages was determined to be 0.2% of CNS. Neither the samples with no CNS additive nor the bovine DNA and the negative control containing 100% swine brain gave any positive signals. The presented results indicate that the real-time RT-PCR assay was just as reproducible between laboratories, as repeatable within a laboratory, could reliably be used for detection of bovine spongiform encephalopathy risk material in meat and meat products, and signify that it may be used with confidence in any laboratory.

scholarly journals Type-specific identification of influenza viruses A, B and C by the polymerase chain reaction

1992 ◽  
Vol 39 (1-2) ◽  
pp. 1-13 ◽  
Author(s):  
E.C.J. Claas ◽  
M.J.W. Sprenger ◽  
G.E.M. Kletera ◽  
R.Van Beek ◽  
W.G.V. Quint ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Influenza Viruses ◽  
Chain Reaction ◽  
Specific Identification ◽  
Polymerase Chain

scholarly journals Development of a polymerase chain reaction assay for specific identification ofClostridium colinum

2008 ◽  
Vol 37 (2) ◽  
pp. 179-181 ◽  
Author(s):  
L. Bano ◽  
I. Drigo ◽  
K. S. Macklin ◽  
S. W. Martin ◽  
R. S. Miller ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Assay ◽  
Chain Reaction ◽  
Specific Identification ◽  
Polymerase Chain

Combination of Immunomagnetic Separation and Polymerase Chain Reaction for the Simultaneous Detection of Listeria monocytogenes and Salmonella spp. in Food Samples

2001 ◽  
Vol 64 (11) ◽  
pp. 1744-1750 ◽  
Author(s):  
HSIEN-YEE HSIH ◽  
HAU-YANG TSEN
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Simultaneous Detection ◽  
Meat Products ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Pcr Method ◽  
Polymerase Chain

A method that combined the immunomagnetic separation (IMS) technique and the multiplex polymerase chain reaction (PCR) method (i.e., the IMS-mPCR method) was developed for simultaneous detection of Listeria monocytogenes and Salmonella spp. in food samples. When only the multiplex PCR method was used, it was found that if cell numbers of each of the two target organisms (L. monocytogenes and Salmonella spp.) were above the detection limit, but differed by more than 2 logs—e.g., n × 107 to n × 104 or n × 106 to n × 103—the organism presenting the lower numbers might go undetected. Following the enrichment step with universal preenrichment (UP) broth, if an IMS method using equal quantities of anti-Listeria and anti-Salmonella immunomagnetic beads was performed prior to PCR, both pathogens could be detected unambiguously. Such results could be obtained for target organisms in food samples, such as milk, dairy, and meat products, if similar enrichment and IMS steps were performed prior to PCR.

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