scholarly journals An Assay for Measuring the Mannan-Binding Lectin Pathway of Complement Activation in Chickens

2007 ◽  
Vol 86 (11) ◽  
pp. 2322-2326 ◽  
Author(s):  
L.R. Norup ◽  
H.R. Juul-Madsen
Keyword(s):  
Complement Activation ◽  
Lectin Pathway ◽  
Mannan Binding Lectin ◽  
Binding Lectin

An assay for the mannan-binding lectin pathway of complement activation

2001 ◽  
Vol 257 (1-2) ◽  
pp. 107-116 ◽  
Author(s):  
S.V Petersen ◽  
S Thiel ◽  
L Jensen ◽  
R Steffensen ◽  
J.C Jensenius
Keyword(s):  
Complement Activation ◽  
Lectin Pathway ◽  
Mannan Binding Lectin ◽  
Binding Lectin

scholarly journals Global Autorecognition and Activation of Complement by Mannan-Binding Lectin in a Mouse Model of Type 1 Diabetes

2017 ◽  
Vol 2017 ◽  
pp. 1-13 ◽  
Author(s):  
Esben Axelgaard ◽  
Jakob Appel Østergaard ◽  
Saranda Haxha ◽  
Steffen Thiel ◽  
Troels Krarup Hansen
Keyword(s):  
Blood Glucose ◽  
Complement Activation ◽  
Ex Vivo ◽  
Vascular Complications ◽  
Lectin Pathway ◽  
Double Knockout ◽  
High Blood Glucose ◽  
New Findings ◽  
Mannan Binding Lectin ◽  
Binding Lectin

Increasing evidence links mannan-binding lectin (MBL) to late vascular complications of diabetes. MBL is a complement-activating pattern recognition molecule of the innate immune system that can mediate an inflammation response through activation of the lectin pathway. In two recent animal studies, we have shown that autoreactivity of MBL is increased in the kidney in diabetic nephropathy. We hypothesize that long-term exposure to uncontrolled high blood glucose in diabetes may mediate formation of neoepitopes in several tissues and that MBL is able to recognize these structures and thus activate the lectin pathway. To test this hypothesis, we induced diabetes by injection of low-dose streptozotocin in MBL double-knockout (MBL/DKO) mice. Development of diabetes was followed by measurements of blood glucose and urine albumin-to-creatinine ratio. Fluorophore-labelled recombinant MBL was injected intravenously in diabetic and nondiabetic mice followed by ex vivo imaging of several organs. We observed that MBL accumulated in the heart, liver, brain, lung, pancreas, and intestines of diabetic mice. We furthermore detected increased systemic complement activation after administration of MBL, thus indicating MBL-mediated systemic complement activation in these animals. These new findings indicate a global role of MBL during late diabetes-mediated vascular complications in various tissues.

Mannan-binding lectin associated serine protease 3 (MASP-3) — a new component of the lectin pathway of complement activation

2000 ◽  
Vol 49 (1-2) ◽  
pp. 79 ◽  
Author(s):  
M.R. Dahl ◽  
S. Thiel ◽  
A.C. Willis ◽  
T. Vorup-Jensen ◽  
T. Christensen ◽  
...  
Keyword(s):  
Serine Protease ◽  
Complement Activation ◽  
Lectin Pathway ◽  
Mannan Binding Lectin ◽  
Binding Lectin
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