scholarly journals Antibody Response to Newcastle Disease Virus and Pasteurella multocida of Two Strains of Turkeys

1988 ◽  
Vol 67 (10) ◽  
pp. 1372-1377 ◽  
Author(s):  
M.M. SHARAF ◽  
K.E. NESTOR ◽  
Y.M. SAIF ◽  
R.E. SACCO ◽  
G.B. HAVENSTEIN
Keyword(s):  
Antibody Response ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Pasteurella Multocida

scholarly journals Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination

2020 ◽  
Vol 45 (2) ◽  
pp. 78-90
Author(s):  
R.W. Astuti ◽  
N. Wijayanti ◽  
A. Haryanto
Keyword(s):  
Antibody Response ◽  
Fusion Protein ◽  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Fusion Protein ◽  
Blue Staining ◽  
F Protein ◽  
Local Isolate ◽  
And Control

This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody response in the broiler sera which were injected with pure recombinant F protein compared with treated broilers that were vaccinated with commercial inactive NDV vaccines and control broilers without vaccination. The results showed that the recombinant F protein of NDV was successfully expressed, purified and visualized by SDS-PAGE with Coomassie Brilliant Blue staining and Westernblotting methods as a specific recombinant F protein with a molecular weight of 28 kDa. The pure recombinant F protein then was injected into broilers to determine the antibody response in broiler serum. Indirect ELISA showed that the production of antibodies was high in F protein vaccinated groups in comparison with other treated and control groups. The recombinant F protein has potential to be developed as a recombinant vaccine candidate after truncating the 6x His-tag part to obtain higher antibody respond if compared with antibody production in broiler serum post vaccinated with some commercially available broiler vaccines.

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