scholarly journals A Method for the Observation of Long Term Development in Chick Embryos

1982 ◽  
Vol 61 (1) ◽  
pp. 172-174 ◽  
Author(s):  
S.B. BARNETT
Keyword(s):  
Chick Embryos

Mineralization during Long-Term Cultivation of Chick Embryos in Vitro

1980 ◽  
Vol 163 (2) ◽  
pp. 249-257 ◽  
Author(s):  
H. C. Slavkin ◽  
M. D. Slavkin ◽  
P. Bringas
Keyword(s):  
Chick Embryos

176 METHOD FOR LONG-TERM CULTURE OF THE PULMONARY ARTERIAL TREE DISSECTED FROM CHICK EMBRYOS.

2004 ◽  
Vol 52 (Suppl 1) ◽  
pp. S109.6-S110
Author(s):  
N. Gundersen ◽  
J. Sweeley ◽  
M. Williams ◽  
M. J. Dahl ◽  
G. C. Schoenwolf ◽  
...  
Keyword(s):  
Chick Embryos ◽  
Arterial Tree ◽  
Long Term Culture ◽  
Pulmonary Arterial

Technique for Liquid Culture of Early Chick Embryos Suitable for Long Term Live Imaging

2015 ◽  
Author(s):  
Cornelis Weijer ◽  
Emil Rozbicki ◽  
Manli Chuai ◽  
Cornelis Jan Weijer
Keyword(s):  
Liquid Culture ◽  
Live Imaging ◽  
Chick Embryos

scholarly journals A single-plasmid approach for genome editing coupled with long-term lineage analysis in chick embryos

Development ◽  
2021 ◽  
pp. dev.193565
Author(s):  
Shashank Gandhi ◽  
Yuwei Li ◽  
Weiyi Tang ◽  
Jens B. Christensen ◽  
Hugo A. Urrutia ◽  
...  
Keyword(s):  
Genome Editing ◽  
Gene Function ◽  
Chick Embryos ◽  
Fluorescent Marker ◽  
Guide Rna ◽  
Single Plasmid ◽  
Lineage Analysis

An important strategy for establishing mechanisms of gene function during development is through mutation of individual genes and analysis of subsequent effects on cell behavior. Here, we present a single-plasmid approach for genome editing in chick embryos to study experimentally perturbed cells in an otherwise normal embryonic environment. To achieve this, we have engineered a plasmid that encodes Cas9 protein, gene-specific guide RNA (gRNA), and a fluorescent marker within the same construct. Using transfection- and electroporation-based approaches, we show that this construct can be used to perturb gene function in early embryos as well as human cell lines. Importantly, insertion of this cistronic construct into replication-incompetent avian (RIA) retroviruses allowed us to couple gene knockouts with long-term lineage analysis. We demonstrate the application of our newly-engineered constructs and viruses by perturbing β-catenin in vitro and Sox10, Pax6, and Pax7 in the neural crest, retina, neural tube and segmental plate in vivo, respectively. Together, this approach enables knocking out genes of interest in identifiable cells in living embryos and can be broadly applied to numerous genes in different embryonic tissues.

METHOD FOR LONG-TERM CULTURE OF THE PULMONARY ARTERIAL TREE DISSECTED FROM CHICK EMBRYOS.

2004 ◽  
Vol 52 ◽  
pp. S109-S110
Author(s):  
N. Gundersen ◽  
J. Sweeley ◽  
M. Williams ◽  
M. J. Dahl ◽  
G. C. Schoenwolf ◽  
...  
Keyword(s):  
Chick Embryos ◽  
Arterial Tree ◽  
Long Term Culture ◽  
Pulmonary Arterial
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