scholarly journals The Phosphorus Excretion Pattern and Balance during One Egg Cycle of the Laying Hen Fed a Phosphorus Deficient Diet with or without a Single Dose of Phosphoric Acid

1979 ◽  
Vol 58 (6) ◽  
pp. 1535-1540 ◽  
Author(s):  
J.H. CHOI ◽  
R.D. MILES ◽  
R.H. HARMS
Keyword(s):  
Single Dose ◽  
Phosphoric Acid ◽  
Laying Hen ◽  
Deficient Diet ◽  
Phosphorus Excretion ◽  
Excretion Pattern

Partition of Calcium and Phosphorus Excretion in the Laying Hen

Nature ◽  
1961 ◽  
Vol 189 (4766) ◽  
pp. 759-760 ◽  
Author(s):  
S. HURWITZ ◽  
P. GRIMINGER
Keyword(s):  
Laying Hen ◽  
Phosphorus Excretion ◽  
Calcium And Phosphorus

scholarly journals Effect of a single dose of dimethylnitrosamine on biosynthesis of nucleic acid and protein in rat liver and kidney

1971 ◽  
Vol 125 (4) ◽  
pp. 943-952 ◽  
Author(s):  
B. W. Stewart ◽  
P. N. Magee
Keyword(s):  
Single Dose ◽  
Rat Liver ◽  
Sucrose Density Gradient ◽  
Polymerase Activity ◽  
Hepatic Necrosis ◽  
Deficient Diet ◽  
Morphological Studies ◽  
Liver And Kidney

1. Administration of a single dose of dimethylnitrosamine to rats temporarily fed on a protein-deficient diet causes a high incidence of kidney tumours. The effect of such a dose of dimethylnitrosamine (40mg/kg body wt.) on metabolism of nucleic acids and protein in rat liver and kidneys was examined during the week immediately after administration. 2. Incorporation of [14C]leucine and [14C]orotate into hepatic macromolecules was inhibited within 5h of injection of dimethylnitrosamine, and did not recover for at least 5 days. Interpretation of these results is complicated by the concomitant extensive hepatic necrosis. 3. Renal RNA synthesis was assayed by incorporation of [14C]orotate in vivo and measurement of DNA-dependent RNA polymerase activity in vitro. Both systems indicate biphasic inhibition; minimal activity was recorded 9h and 3 days after treatment. Changes in incorporation of [14C]leucine into renal protein were similar but less marked. 4. Sucrose-density-gradient analysis of renal cytoplasmic RNA indicated increased synthesis of rRNA 24h after injection of the nitrosamine. The rate of loss of radioactivity from kidney ribosomes pre-labelled with [14C]orotate was not modified by dimethylnitrosamine. 5. Dimethylnitrosamine increased incorporation of [3H]-thymidine into renal DNA. The three distinct periods of stimulated synthesis observed are discussed, with particular reference to recently published morphological studies of the sequential development of kidney tumours induced by dimethylnitrosamine in protein-depleted rats.

The partition of certain mineral nutrients in the colostomised laying pullet and the determination of faecal endogenous calcium and phosphorus excretion by an isotopic dilution method

1965 ◽  
Vol 64 (3) ◽  
pp. 305-310 ◽  
Author(s):  
W. O. Brown ◽  
K. J. McCracken
Keyword(s):  
Mineral Nutrients ◽  
Laying Hen ◽  
Isotopic Dilution ◽  
Dilution Method ◽  
Sodium Potassium ◽  
Isotopic Dilution Method ◽  
Phosphorus Excretion ◽  
Calcium Phosphorus ◽  
Calcium And Phosphorus

1. An experiment is described in which the partition of calcium phosphorus, sodium, potassium and manganese in the excreta of the colostomized laying hen fed a diet adequate in these minerals is examined.

Phosphorus homeostasis in sheep. I. Effects of ligation of parotid salivary ducts

1974 ◽  
Vol 25 (3) ◽  
pp. 475 ◽  
Author(s):  
FM Tomas ◽  
M Somers
Keyword(s):  
Salivary Secretion ◽  
Urinary Output ◽  
Reverse Direction ◽  
Deficient Diet ◽  
Phosphorus Excretion ◽  
Parotid Duct ◽  
Duct Ligation ◽  
Urinary Phosphorus Excretion ◽  
Phosphorus Levels ◽  
Plasma Phosphorus

The effects of bilateral parotid duct ligation upon calcium and phosphorus concentrations in blood plasma and upon phosphorus excretion in urine and faeces have been examined. Five Merino wethers were used, of which three were given a phosphorus-deficient diet (0.304 g phosphorus/day) and two a phosphorus-sufficient diet (1.454 g phosphorus/day) for 32 days prior to ligation of the parotid ducts. Blood samples were taken every 6 hours for 2 days before and 4 days after parotid duct ligation followed by less frequent sampling for a further 3 days. Parotid duct ligation caused an increase in plasma inorganic phosphorus levels after the first day in the phosphorus-supplemented sheep and in one of the deficient animals. Plasma calcium concentrations tended to be inversely related to plasma phosphorus levels and showed marked variations on the fourth day following duct ligation, coinciding with a sudden fall in plasma phosphorus levels of 30-50 %. Urinary phosphorus excretion in the phosphorus-sufficient animals was increased 20 to 50-fold following ligation and reached a peak of about 600 mg/day on the fourth day, but there was no significant change in phosphorus balance. Marked alterations in the daily urinary output of phosphorus tended to be associated with a change in plasma phosphorus levels in the reverse direction. Four of the sheep were re-examined 22 months after duct ligation and the elevated urinary output of phosphorus was found to have persisted in three of the four animals. It appears that there is a relationship between the salivary secretion of phosphorus to the gut and the urinary phosphorus excretion which contributes towards maintenance of the phosphorus homeostasis in sheep.

THE USE OF P32 LABELED GLYCEROPHOSPHATE IN THE STUDY OF YOLK PHOSPHOLIPIDS

1949 ◽  
Vol 27b (7) ◽  
pp. 629-637 ◽  
Author(s):  
J. W. T. Spinks ◽  
C. C. Lee ◽  
J. B. O'Neil
Keyword(s):  
Enzymatic Hydrolysis ◽  
Phosphoric Acid ◽  
Comparative Studies ◽  
Inorganic Phosphate ◽  
Laying Hen ◽  
Trisodium Phosphate ◽  
Dihydrogen Phosphate ◽  
Sodium Dihydrogen Phosphate ◽  
Sodium Dihydrogen ◽  
Hydrolysis Of

Labeled sodium glycerophosphate has been prepared by phosphorylating glycerol with sodium dihydrogen phosphate containing P32. After the labeled sodium glycerophosphate had been fed to a laying hen, radiophosphorus from glycerophosphate appeared in the shells, whites, and yolks of the eggs. The uptake in different eggs followed a pattern similar to that found for inorganic phosphate, the maximum occurring at the fourth or fifth egg. Comparative studies using P32 labeled sodium glycerophosphate and trisodium phosphate indicate that the rate of utilization of the phosphorus from glycerophosphate is less than the rate of utilization of the phosphorus from inorganic phosphate. The possibility of an enzymatic hydrolysis of glycerophosphate followed by the incorporation of the resulting phosphoric acid into the phospholipids and the phosphorylation of lower glycerides (partially hydrolyzed fat) by inorganic phosphate as a mechanism for phospholipid formation is discussed.

scholarly journals Excretion pattern of aflatoxin M1 in milk of goats fed a single dose of aflatoxin B1

2012 ◽  
Vol 95 (5) ◽  
pp. 2656-2661 ◽  
Author(s):  
G. Battacone ◽  
A. Nudda ◽  
S.P.G. Rassu ◽  
M. Decandia ◽  
G. Pulina
Keyword(s):  
Single Dose ◽  
Aflatoxin B1 ◽  
Aflatoxin M1 ◽  
Excretion Pattern

Regulation of renal vitamin D hydroxylase activity in vitamin D deficient rats

1985 ◽  
Vol 63 (8) ◽  
pp. 978-982 ◽  
Author(s):  
M. Warner ◽  
A. Tenenhouse
Keyword(s):  
Vitamin D ◽  
Enzyme Activity ◽  
Single Dose ◽  
Calcium Concentration ◽  
Male Rats ◽  
Hydroxylase Activity ◽  
Deficient Diet ◽  
Serum Calcium Concentration ◽  
Pth Resistance ◽  
Detectable Serum

The regulation of renal mitochondrial 1-hydroxylase activity in chronic vitamin D deficiency was studied in male rats. These rats were born of mothers who had been raised from weaning (21 days) on a vitamin D deficient diet and who had no detectable serum 1,25-dihydroxycholecalciferol (1,25-(OH)2D) at the time their offspring were weaned (28 days). In the pups, renal mitochondrial 1-hydroxylase activity was undetectable before the 3rd week of life even though the animals were severely hypocalcemic from birth. The 1-hydroxylase activity first became detectable at 26 days of age, rapidly reached a maximum at day 34, then decreased to become undetectable again by 65 days. Throughout this time serum calcium concentration was <5.0 mg/dL and serum parathyroid hormone (PTH) concentration, measured by a midmolecule radioimmunoassay, was two-to five-fold greater than that found in vitamin D replete rats. 1-Hydroxylase activity could be restored in the +65-day-old animals by administration of a single dose of 2.5 μg vitamin D3. Enzyme activity was detected within 24 h, was maximal at 72 h, and returned to undetectable levels by 96 h after administration of the vitamin. Serum 1,25-(OH)2D which was undetectable before administration of the vitamin D3, was 108 and 458 pg/mL at 16 and 40 h, respectively, after the injection. The serum concentration of this metabolite then decreased progressively to 80 pg/mL by 6 days. 24-Hydroxylase activity first became detectable 48 h after vitamin D administration, increased to a maximum at 96 h, and thereafter decreased to become undetectable by 7 days. The urinary excretion of phosphate and cyclic AMP was 10% of control values between 65 and 90 days of age. These values became normal 4 days after a single dose of 2.5 μg vitamin D3. From these data it is concluded that there are two distinct levels of regulation of 1-hydroxylase activity: a vitamin D independent induction of the activity at the time of weaning that is transient and is not associated with any detectable 24-hydroxylase activity; and the second is a vitamin D dependent induction of enzyme activity seen in animals which prior to administration of the vitamin manifest the characteristics of PTH resistance and have no detectable renal hydroxylase activity. The mechanisms of these effects remain to be determined.

The Response of Testes Cells to Low Level, Single dose Helium Particle Irradiation

1982 ◽  
Vol 40 ◽  
pp. 252-253
Author(s):  
D.E. Philpott ◽  
W. Sapp ◽  
C. Williams ◽  
J. Stevenson ◽  
S. Black ◽  
...  
Keyword(s):  
Stem Cell ◽  
Single Dose ◽  
B Cell ◽  
Cell Survival ◽  
Spermatogonial Stem Cell ◽  
Low Doses ◽  
Particle Irradiation ◽  
Stem Cell Survival ◽  
Irradiation Injury ◽  
Radio Sensitivity

Spermatogonial stem-cell survival after irradiation injury has been studied in rodents by histological counts of surviving cells. Many studies, including previous work from our laboratory, show that the spermatogonial population demonstrates a heterogeneous response to irradiation. The spermatogonia increase in radio-sensitivity as differentiation proceeds through the sequence As - Apr - A1 - A2 - A3 - A4 - In - B. The stem (As) cell is the most resistant and the B cell is the most sensitive. The purpose of this work is to investigate the response of spermatogonial cell to low doses (less than 10 0 rads) of helium particle irradiation.

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