scholarly journals Effects of Early Infectious Bursal Disease Virus Infection on Immunity to Newcastle Disease in Adult Chickens

1979 ◽  
Vol 58 (4) ◽  
pp. 794-798 ◽  
Author(s):  
J.J. GIAMBRONE
Keyword(s):  
Virus Infection ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals Western blot detection of infectious bursal disease virus infection

1998 ◽  
Vol 31 (5) ◽  
pp. 671-674 ◽  
Author(s):  
S.R.F.G. Pereira ◽  
C.E.P.F. Travassos ◽  
A. Huguenim ◽  
A.C.C. Guimarães ◽  
A.G. Silva ◽  
...  
Keyword(s):  
Virus Infection ◽  
Western Blot ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Infectious Bursal Disease ◽  
Bursal Disease

scholarly journals A Recombinant Newcastle Disease Virus (NDV) Expressing VP2 Protein of Infectious Bursal Disease Virus (IBDV) Protects against NDV and IBDV

2004 ◽  
Vol 78 (18) ◽  
pp. 10054-10063 ◽  
Author(s):  
Zhuhui Huang ◽  
Subbiah Elankumaran ◽  
Abdul S. Yunus ◽  
Siba K. Samal
Keyword(s):  
Newcastle Disease Virus ◽  
Disease Virus ◽  
Newcastle Disease ◽  
Recombinant Virus ◽  
Infectious Bursal Disease Virus ◽  
Antibody Responses ◽  
Infectious Bursal Disease ◽  
Vp2 Protein ◽  
Bursal Disease ◽  
Recombinant Newcastle Disease Virus

ABSTRACT Infectious bursal disease virus (IBDV) causes a highly immunosuppressive disease in chickens. Currently available, live IBDV vaccines can lead to generation of variant viruses. We have developed an alternative vaccine that will not create variant IBDV. By using the reverse genetics approach, we devised a recombinant Newcastle disease virus (NDV) vector from a commonly used vaccine strain LaSota to express the host-protective immunogen VP2 of a variant IBDV strain GLS-5. The gene encoding the VP2 protein of the IBDV was inserted into the most 3′-proximal locus of a full-length NDV cDNA for high-level expression. We successfully recovered the recombinant virus, rLaSota/VP2. The rLaSota/VP2 was genetically stable, at least up to 12 serial passages in chicken embryos, and was shown to express the VP2 protein. The VP2 protein was not incorporated into the virions of recombinant virus. Recombinant rLaSota/VP2 replicated to a titer similar to that of parental NDV strain LaSota in chicken embryos and cell cultures. To assess protective efficacy of the rLaSota/VP2, 2-day-old specific-pathogen-free chickens were vaccinated with the recombinant virus and challenged with a highly virulent NDV strain Texas GB or IBDV variant strain GLS-5 at 3 weeks postvaccination. Vaccination with rLaSota/VP2 generated antibody responses against both NDV and IBDV and provided 90% protection against NDV and IBDV. Booster immunization induced higher levels of antibody responses against both NDV and IBDV and conferred complete protection against both viruses. These results indicate that the recombinant NDV can be used as a vaccine vector for other avian pathogens.

scholarly journals Nonstructural Protein of Infectious Bursal Disease Virus Inhibits Apoptosis at the Early Stage of Virus Infection

2006 ◽  
Vol 80 (7) ◽  
pp. 3369-3377 ◽  
Author(s):  
Meihong Liu ◽  
Vikram N. Vakharia
Keyword(s):  
Virus Infection ◽  
Dna Fragmentation ◽  
Disease Virus ◽  
Infectious Bursal Disease Virus ◽  
Early Stage ◽  
Nonstructural Protein ◽  
Infectious Bursal Disease ◽  
Knockout Mutant ◽  
Bursal Disease ◽  
Induced Apoptosis

ABSTRACT Infectious bursal disease virus (IBDV), the causative agent of a highly contagious disease in chickens, carries a small nonstructural protein (NS). This protein has been implicated to play a role in the induction of apoptosis. In this study, we investigate the kinetics of viral replication during a single round of viral replication and examine the mechanism of IBDV-induced apoptosis. Our results show that it is caspase dependent and activates caspases 3 and 9. Nuclear factor kappa B (NF-κB) is also activated and is required for IBDV-induced apoptosis. The NF-κB inhibitor MG132 completely inhibited IBDV-induced DNA fragmentation, caspase 3 activation, and NF-κB activation. To study the function of the NS protein in this context, we generated the recombinant rGLS virus and an NS knockout mutant, rGLSNSΔ virus, using reverse genetics. Comparisons of the replication kinetics and markers for virally induced apoptosis indicated that the NS knockout mutant virus induces earlier and increased DNA fragmentation, caspase activity, and NF-κB activation. These results suggest that the NS protein has an antiapoptotic function at the early stage of virus infection.

Sign in / Sign up

Export Citation Format

Share Document