scholarly journals Genotypic frequency of dynamin 1 mutation in service dog breeding colonies in Japan

2016 ◽  
Vol 10 (1) ◽  
pp. 24-27
Author(s):  
Hiroshi Suzuki ◽  
Sakimi Yokozawa ◽  
Yuki Hashimoto ◽  
Misaki Ito ◽  
Koto Mizukami ◽  
...  
Keyword(s):  
Genotypic Frequency ◽  
Service Dog ◽  
Dog Breeding

scholarly journals Elimination of chlamydia in the service dog breeding nursery of the FPS of Russia Main Department in the Sverdlovsk Region

2021 ◽  
Vol 231 (8) ◽  
pp. 67-74
Author(s):  
ANATOLIY D. ALEKSEEV ◽  
◽  
OLGA G. PETROVA ◽  
IGOR M. MILSTEIN ◽  
◽  
...  
Keyword(s):  
Federal Budget ◽  
Service Dogs ◽  
Chain Reaction ◽  
Service Dog ◽  
Penal Institutions ◽  
The Subject ◽  
Polymerase Chain ◽  
Key Words Chlamydia ◽  
Positive Effect ◽  
Dog Breeding

The article studies the elimination of chlamydia in the service dog breeding nursery Federal Budget Institution KP-66 of the FPS of Russia Main Department in the Sverdlovsk Region. The subject of the research is service breeding dogs and puppies kept in a breeding nursery of service dogs. The aim of the research is to develop the most rational treatment regime for chlamydia in dogs. The methodological basis of the research was formed by statistical and pathomorphological methods and diagnostics by the method of polymerase chain reaction (PCR). As a result of the studies, the most optimal treatment scheme for chlamydia in service dogs was selected. The data given in the article will have a positive effect on the epizootic situation in the institutions of the penal system. Conclusions have been made on the need to conduct annual studies of pedigree dogs, as well as service dogs accepted for service in penal institutions, for chlamydia, mycoplasmosis and brucellosis, and, if necessary, for other infections. Key words: chlamydia of dogs, veterinary service of the Federal Penitentiary Service of Russia, infectious diseases of service dogs.

scholarly journals Revealing and identification of isoniazid metabolites in beef meat in case of poisoning of service dogs

2021 ◽  
pp. 7-9
Author(s):  
A. M. Lunegov ◽  
A. G. Ovsyannikov ◽  
I. V. Lunegova
Keyword(s):  
Acute Poisoning ◽  
Leningrad Region ◽  
Toxic Substances ◽  
Service Dogs ◽  
Liquid Chromatograph ◽  
Toxic Compounds ◽  
Service Dog ◽  
Metabolic Products ◽  
Food And Feed ◽  
Dog Breeding

Relevance. Every year, dog breeders are faced with the problem of acute poisoning of dogs. Various toxic compounds can be the cause of dog poisoning. The huge variety of potentially toxic substances makes it difficult to identify the source of poisoning. Service dog breeding also faces this problem, as a result of which it is necessary to have information about the possibility of poisoning service dogs, as well as methods for detecting toxic substances and preventing further poisoning. In service dog breeding, they mainly use feed made by cooking gruel soup in boilers in the feed kitchens of nurseries. Due to the fact that there were cases of poisoning of service dogs in the Vyborg district of the Leningrad region, we conducted research to identify toxic compounds in beef from which feed was made in the feed kitchens of nurseries.Methods. The studies were carried out at the Institute of Toxicology of the Federal Medical and Biological Agency using an Acguity UPLC I-class ultra-performance liquid chromatograph with a spectrophotometric detecto and an Acguity UPLC H-class ultraperformance liquid chromatograph with a Xevo TQD tandem mass spectrometer. Sample components were identified by electronic spectra and mass numbers.Results. According to the results of the study, the presence of isoniazid metabolic products in the form of isonicatinic acid, as well as conjugates of isoniazid with sulfuric, acetic and glucuronic acids, was found in beef meat.The obtained results of the study of the chromatographic profiles of aqueous extracts and the chemical identification of their components in the products that are used for the preparation of food for service dogs make it possible to accurately determine and identify toxic substances with a wide variety of them. Thus, in order to prevent the loss of service dogs, it is necessary to control the newly received food and feed in order to prevent poisoning.

scholarly journals Psychophysiological compatibility of a specialist cynologist and service dog

2019 ◽  
Vol 13 (3) ◽  
pp. 416-422
Author(s):  
E. A. Kusakina ◽  
◽  
R. V. Malchikov ◽  
Keyword(s):  
Dynamic Characteristics ◽  
Penal System ◽  
Direct Influence ◽  
Joint Performance ◽  
Service Dog ◽  
Empirical Material ◽  
Dog Breeding

The article discusses the results of the study devoted to one of the most important aspects of service dog breeding – the psychophysiological compatibility of a specialist cynologist and a dog. The main methods for collecting empirical material were the system of checking the dynamic characteristics of ARAKS and the methodology for determining the characteristics of the temperament of cynologists, developed by H. Eysenck. The results of the study emphasize the direct influence of the compatibility of the temperament of the cynologist and the service dog on the results of training and joint performance of service tasks. In institutions of the penal system in order to increase the effectiveness of the cynological service it is advisable to take into account the psychophysiological features in the formed cynologist-dog pairs.

scholarly journals Impact of rs2414096 polymorphism of CYP19 gene on susceptibility of polycystic ovary syndrome and hyperandrogenism in Kashmiri women

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Sairish Ashraf ◽  
Shayaq Ul Abeer Rasool ◽  
Mudasar Nabi ◽  
Mohd Ashraf Ganie ◽  
Shariq R. Masoodi ◽  
...  
Keyword(s):  
Polycystic Ovary Syndrome ◽  
Polycystic Ovary ◽  
Clinical Manifestations ◽  
Additive Model ◽  
Genotypic Frequency ◽  
Ovary Syndrome ◽  
Significant Difference ◽  
Cyp19 Gene ◽  
Reproductive Endocrine ◽  
The Impact

AbstractPolycystic ovary syndrome (PCOS) is the most common reproductive endocrine disorder in pre-menopausal women having complex pathophysiology. Several candidate genes have been shown to have association with PCOS. CYP19 gene encodes a key steroidogenic enzyme involved in conversion of androgens into estrogens. Previous studies have reported contradictory results with regard to association of SNP rs2414096 in CYP19 gene with PCOS and hyperandrogenism in different ethnic populations. Present study was aimed to investigate the impact of SNP rs2414096 polymorphism of CYP19 gene on susceptibility of PCOS and hyperandrogenism in Kashmiri women. Further we also studied the genotypic-phenotypic association for various clinical and biochemical parameters of this polymorphism. Case control study. 394 PCOS cases diagnosed on the basis of Rotterdam criteria and age matched 306 healthy women. We found a significant differences in genotypic frequency (χ2 = 18.91, p < 0.05) as well as allele frequency (OR 0.63, CI 0.51–0.78, χ2 = 17.66, p < 0.05) between PCOS women and controls. The genotype–phenotype correlation analysis showed a significant difference in FG score (p = 0.047) and alopecia (p = 0.045) between the three genotypes. Also, the androgen excess markers like DHEAS (p < 0.001), Androstenedione (p < 0.001), Testosterone (p < 0.001) and FAI (p = 0.005) were significantly elevated in GG genotype and showed a significant difference in additive model in PCOS women. rs2414096 polymorphism of CYP19 gene is associated with the risk of PCOS as well as with clinical and biochemical markers of hyperandrogenism, hence suggesting its role in clinical manifestations of PCOS in Kashmiri women.

scholarly journals AB0117 HLA B27*SUBTYPES FREQUENCIES IN COLOMBIAN PATIENTS WITH SPONDYLOARTHRITIS AND HEALTHY SUBJECTS TYPED IN HIGH-RESOLUTION TECHNIQUE

2020 ◽  
Vol 79 (Suppl 1) ◽  
pp. 1358.1-1359
Author(s):  
C. Romero-Sánchez ◽  
W. Bautista-Molano ◽  
Y. M. Chamorro-Melo ◽  
A. Beltrán-Ostos ◽  
J. De Avila ◽  
...  
Keyword(s):  
High Resolution ◽  
Allelic Frequency ◽  
Male Gender ◽  
Inflammatory Back Pain ◽  
Genotypic Frequency ◽  
Hla B27 ◽  
Healthy Individuals ◽  
Current Smoking ◽  
Subtype B ◽  
The Mean

Background:HLA-B*27 has been identify as a susceptibility and prognostic factor associated to axial spondyloarthritis. HLA-B*27 allele has been described to be present in about 90% of patients with ankylosing spondylitis, and with a different frequency in patients with other subtypes of SpA. In contrast, this allele has been observed to be present only in 7–8% in general population. A remarkable heterogeneity in HLA-B*27 alleles has been reported. They have been determined at DNA sequence and some subtypes have been associated increasing the risk to develop the diseaseObjectives:To establish the frequencies of HLA-B27 subtypes in a group of Colombian patients with SpA and healthy populationMethods:In total, 61 Blood samples from Colombian mestizo individuals with SpA according to ASAS classification-criteria were evaluated by Sequencing Technology: Illumina Sequencing/PacBio Sequencing with analysis of the second and third exon. Results reported with six digits (including null alleles). In total, 294 results of peripheral blood from healthy individuals without joint symptoms were analyzed. Frequencies were obtained for demographic and genetic variables. Ethic Committee approval code 2018-020/2017-023Results:The SpA group had a mean age of 45,88 ± 11,67, 62.3% of them were male, 6.6% reported current smoking and 37.7% reported smoking sometime in life. In total, 67.2% had inflammatory back pain, 14.8% had dactylitis, 63.9% enthesitis and 57.4% arthritis. Thirty patients were HLA-B*27 positive with a genotypic frequency of 50.8% and an allelic frequency of 24.6%. In this group of patients, the mean age was 43,5 ± 11,8, 76.6% were male, 86.7% of them were subtype B*27:05:02g and 13.3% presented the B27:02:01g. None of the SpA patients had both B*27 alleles.On the other hand, the healthy individuals were men in 51.0% and the mean age was 37±15.4 years. Ten subjects were positive for the HLA-B*27 allele with a genotypic frequency of 3.4% and an allelic frequency of 1.7%. In this group of individuals 50.0% were male gender with a mean age of 38.4±17.9. No individuals were found to have the two alleles or homozygous for the B*27 allele. In all of them the subtype B*27:05:02g was observed in high-resolution sequencingConclusion:The SpA group had a mean age of 45,88 ± 11,67, 62.3% of them were male, 6.6% reported current smoking and 37.7% reported smoking sometime in life. In total, 67.2% had inflammatory back pain, 14.8% had dactylitis, 63.9% enthesitis and 57.4% arthritis. Thirty patients were HLA-B*27 positive with a genotypic frequency of 50.8% and an allelic frequency of 24.6%. In this group of patients, the mean age was 43,5 ± 11,8, 76.6% were male, 86.7% of them were subtype B*27:05:02g and 13.3% presented the B27:02:01g. None of the SpA patients had both B*27 alleles.On the other hand, the healthy individuals were men in 51.0% and the mean age was 37±15.4 years. Ten subjects were positive for the HLA-B*27 allele with a genotypic frequency of 3.4% and an allelic frequency of 1.7%. In this group of individuals 50.0% were male gender with a mean age of 38.4±17.9. No individuals were found to have the two alleles or homozygous for the B*27 allele. In all of them the subtype B*27:05:02g was observed in high-resolution sequencingAcknowledgments:Hospital Militar Central (Grant 2017-023/2018-020), the Government Institute of Science, Technology, and Innovation, Francisco Jose de Caldas—COLCIENCIAS (Grant No. 130877757442) and Colombian Rheumatology Association (Grant-Conv-2019)Disclosure of Interests:None declared

scholarly journals Genetic Diversity of CD14 Promoter Gene Polymorphism (rs2569190) is Associated With Regulation of Malaria Parasitemia and Susceptibility to Plasmodium falciparum Infection

2017 ◽  
Vol 10 ◽  
pp. 117863361772678 ◽  
Author(s):  
Olusola Ojurongbe ◽  
Roland I Funwei ◽  
Tara J Snyder ◽  
Najihah Aziz ◽  
Yi Li ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Plasmodium Falciparum ◽  
Plasmodium Falciparum Malaria ◽  
Cell Types ◽  
Infected Group ◽  
Genotypic Frequency ◽  
Experimental Cerebral Malaria ◽  
Asymptomatic Patients ◽  
Mutant Variant ◽  
Promoter Gene

CD14 is a multifunctional receptor expressed on many cell types and has been shown to mediate immune response resulting in the activation of an inflammatory cascade, with polymorphism of its promoter (rs2569190) found to be associated with susceptibility to several diseases. In malaria infection, the CD14 gene demonstrated a pathogenic profile in regulating experimental cerebral malaria, with reports of elevated levels of soluble CD14 in serum of patients but no definitive conclusion. We present a detailed analysis of genetic diversity of CD14 promoter gene (snp −159 C/T; rs2519190) polymorphism between a malaria-infected group and uninfected controls and its association with clinical parameters of disease. Genomic DNA samples obtained from 106 Plasmodium falciparum malaria–infected patients and 277 uninfected controls were elucidated with a polymerase chain reaction-restriction fragment length polymorphism (RFLP) assay. Our results show a significant diversity ( P = 3.32E−06) in the genotypic frequency (3.8% versus 22.4%) of the rs2569190 mutant variant between the malaria-infected group and controls, respectively. The mutant allele had the lowest frequency among the malaria-infected group demonstrating its necessity for infection. Mean parasitemia (parasites/μL of blood) was significantly regulated based on CD14 polymorphic profile (19 855 versus 37 041 versus 49 396 for homozygote mutants, heterozygotes, and homozygote wild type, respectively). Interestingly, we found no association between CD14 genetic variants with fever, age of patients, or anemia. How this affects disease severity between subregional and continental groups deserves further clarification, including extending these studies in a larger group and among severe and asymptomatic patients with malaria.

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