scholarly journals Antitumor Effect of IL-2 and TRAIL Proteins Expressed by Recombinant Salmonella in Murine Bladder Cancer Cells

2021 ◽  
Vol 55 (4) ◽  
pp. 460-476
Keyword(s):  
Oxidative Stress ◽  
Bladder Cancer ◽  
Cell Membrane ◽  
Cell Viability ◽  
Cancer Cells ◽  
Cell Morphology ◽  
Antitumor Effect ◽  
Interleukin 2 ◽  
Continuous Increase ◽  
Bladder Cancer Cells

Background/Aims: Cancer is the second most deadly disease in the world. The bladder cancer is one of the most aggressive types and shows a continuous increase in the number of cases. The use of bacteria as live vectors to deliver molecules directly to the tumor is a promising tool and has been used as an adjuvant treatment against several types of cancer. The aim of this study was to investigate the antitumor effect of Interleukin 2 (IL-2), TNF-related apoptosis-inducing ligand (TRAIL) and protein MIX against murine bladder cancer cells, lineage MB49. Methods: The attenuated Salmonella strain SL3261 was transformed by inserting the IL-2 and TRAIL genes. The effects of proteins on cell viability (MTT method), cell morphology (optical microscopy), cell recovery (clonogenic assay), cell membrane (lactate dehydrogenase release - LDH), on oxidative stress pathway (levels of nitric oxide, NO) and apoptosis (flow cytometry and high resolution epifluorescence images) were evaluated at intervals of 24 and 48 hours of action. Results: The results showed that there was a decrease in cell viability via damage to the cell membrane, alteration of cell morphology, non-recovery of cells, increase in the production of NO and incubate for of cells in the state of apoptosis in the two periods analyzed. Conclusion: The data presented suggest that IL-2, TRAIL and their MIX proteins in MB49 cells have cytotoxic potential and that this is associated with oxidative stress and apoptosis pathways. These results may contribute to the development of new therapeutic strategies for bladder cancer.

792: Role of Oxidative Stress in Tumorigenic Potential of Bladder Cancer Cells

2005 ◽  
Vol 173 (4S) ◽  
pp. 214-215 ◽  
Author(s):  
Daniel Cho ◽  
Xiao Fang Ha ◽  
J. Andre Melendez ◽  
Louis J. Giorgi ◽  
Badar M. Mian
Keyword(s):  
Oxidative Stress ◽  
Bladder Cancer ◽  
Cancer Cells ◽  
Tumorigenic Potential ◽  
Bladder Cancer Cells

scholarly journals Chemosensitizing effect of maitake mushroom extract on carmustine cytotoxicity in human bladder cancer cells

2020 ◽  
Vol 8 (9) ◽  
pp. 3154
Author(s):  
Joel Hillelsohn ◽  
Michael Stern ◽  
Mina Iskander ◽  
Muhammad Choudhury ◽  
Sensuke Konno
Keyword(s):  
Oxidative Stress ◽  
Bladder Cancer ◽  
Cell Viability ◽  
Cancer Cells ◽  
Anticancer Drugs ◽  
Therapeutic Option ◽  
Human Bladder Cancer ◽  
Human Bladder ◽  
Mushroom Extract ◽  
T24 Cells

Background: Despite several therapeutic options available for bladder cancer, the outcomes are less satisfactory.  To find a more effective modality, we were interested in the bioactive mushroom extract, PDF, which has been shown to sensitize certain anticancer drugs.  Accordingly, we investigated if cytotoxic effects of several anticancer drugs used on bladder cancer patients could be enhanced with PDF in vitro.Methods: Human bladder cancer T24 cells were treated with four anticancer drugs, carmustine (BCNU), 5-fluorouracil (5FU), cisplatin (CPL), and doxorubicin (DOX) alone, their combinations, or in combination with PDF, and cell viability was determined.  To explore the anticancer mechanism, the status of glyoxalase I (Gly-I), an enzyme involved in the drug resistance of cancer cells, and oxidative stress that can cause severe cellular injury/damage was also assessed.Results: BCNU and 5FU alone resulted in a >50% reduction in cell viability but CPL and DOX had no such effects.  Only a combination of BCNU and PDF led to a drastic (~90%) cell viability reduction, accompanied by inactivation of Gly-I and an increase in oxidative stress.  However, any combinations of other drugs and PDF had little effects on cell viability, Gly-I activity, or severity of oxidative stress.Conclusions: This study shows that anticancer activity of BCNU is significantly potentiated with PDF in T24 cells.  This is rather attributed to inactivated Gly-I and increased oxidative stress.  Therefore, PDF appears to have a chemosensitizing effect capable of enhancing BCNU cytotoxicity, which may offer an alternative, improved therapeutic option for bladder cancer.

Replication-competent adenovirus expressing TRAIL synergistically potentiates the antitumor effect of gemcitabine in bladder cancer cells

Tumor Biology ◽  
2014 ◽  
Vol 35 (6) ◽  
pp. 5937-5944 ◽  
Author(s):  
Lijun Mao ◽  
Chunhua Yang ◽  
Liantao Li ◽  
Lanzhou Nai ◽  
Li Fan ◽  
...  
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Antitumor Effect ◽  
Bladder Cancer Cells

THE ANTITUMOR EFFECT OF γδT CELLS AGAINST BLADDER CANCER CELLS IS ENHANCED FOLLOWING UPREGULATION OF NKG2D LIGANDS BY VALPROIC ACID

2009 ◽  
Vol 181 (4S) ◽  
pp. 348-348
Author(s):  
Toru Suzuki ◽  
Bishnu Acharya ◽  
Shuji Terao ◽  
Shingo Yamamoto ◽  
Hiroki Shima ◽  
...  
Keyword(s):  
Valproic Acid ◽  
Bladder Cancer ◽  
Cancer Cells ◽  
Antitumor Effect ◽  
Γδt Cells ◽  
Nkg2d Ligands ◽  
Bladder Cancer Cells

scholarly journals Oxidative Stress Sensitizes Bladder Cancer Cells to TRAIL Mediated Apoptosis by Down-Regulating Anti-Apoptotic Proteins

2009 ◽  
Vol 182 (3) ◽  
pp. 1178-1185 ◽  
Author(s):  
Shai J. White-Gilbertson ◽  
Laura Kasman ◽  
John McKillop ◽  
Tejas Tirodkar ◽  
Ping Lu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bladder Cancer ◽  
Cancer Cells ◽  
Bladder Cancer Cells ◽  
Apoptotic Proteins

Antitumor effect of a novel nuclear factor-κB activation inhibitor in bladder cancer cells

2003 ◽  
Vol 3 (6) ◽  
pp. 793-798 ◽  
Author(s):  
Yutaka Horiguchi ◽  
Kenji Kuroda ◽  
Jun Nakashima ◽  
Masaru Murai ◽  
Kazuo Umezawa
Keyword(s):  
Bladder Cancer ◽  
Cancer Cells ◽  
Antitumor Effect ◽  
Nuclear Factor Κb ◽  
Nuclear Factor ◽  
Bladder Cancer Cells
Sign in / Sign up

Export Citation Format

Share Document