Hair Sciences: Hair Follicle generation in a Mouse Model Using a Combination of Human Dermal Papilla and Follicular Epithelial Cells

Abstract Background Hair follicle (HF) development and growth are dependent on epithelial-mesenchymal interactions (EMIs). Dermal papilla (DP) cells are recognized as the key inductive mesenchymal player, but the ideal source of receptive keratinocytes for human HF regeneration is yet to be defined. We herein investigated whether human interfollicular epidermal keratinocytes with stem-like features (EpSlKCs), characterized by a α6bri/CD71dim expression, can replace human hair follicular keratinocytes (HHFKCs) for the recreation of the HF epithelium and respective EMIs. Methods The α6bri/CD71dim cellular fraction was selected from the whole interfollicular keratinocyte population through fluorescence-activated cell sorting and directly compared with follicular keratinocytes in terms of their proliferative capacity and phenotype. The crosstalk with DP cells was studied in an indirect co-culture system, and EpSlKC hair forming capacity tested in a hair reconstitution assay when combined with DP cells. Results EpSlKCs exhibited a phenotypic profile similar to follicular keratinocytes and were capable of increasing DP cell proliferation and, for short co-culture times, the number of alkaline phosphatase-active cells, suggesting an improvement of their inductivity. Moreover, the recreation of immature HFs and sebaceous glands was observed after EpSlKC and DP cell co-grafting in nude mice. Conclusions Our results suggest that EpSlKCs are akin to follicular keratinocytes and can crosstalk with DP cells, contributing to HF morphogenesis in vivo, thus representing an attractive epithelial cell source for hair regeneration strategies.

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Effect of Slit/Robo signaling on regeneration in lung emphysema

Experimental & Molecular Medicine ◽

10.1038/s12276-021-00633-8 ◽

2021 ◽

Author(s):

Jin-Soo Park ◽

RyeonJin Cho ◽

Eun-Young Kang ◽

Yeon-Mok Oh

Keyword(s):

Epithelial Cells ◽

Mouse Model ◽

Lung Epithelial Cells ◽

Mouse Lung ◽

Chronic Obstructive ◽

Irreversible Damage ◽

Lung Epithelial ◽

And Migration ◽

Proliferation And Migration

AbstractEmphysema, a pathological component of chronic obstructive pulmonary disease, causes irreversible damage to the lung. Previous studies have shown that Slit plays essential roles in cell proliferation, angiogenesis, and organ development. In this study, we evaluated the effect of Slit2 on the proliferation and migration of mouse lung epithelial cells and its role in regeneration in an emphysema lung mouse model. Here, we have shown that Slit2/Robo signaling contributes to the regeneration of lungs damaged by emphysema. Mouse epithelial lung cells treated with Slit2 exhibited increased proliferation and migration in vitro. Our results also showed that Slit2 administration improved alveolar regeneration in the emphysema mouse model in vivo. Furthermore, Slit2/Robo signaling increased the phosphorylation of ERK and Akt, which was mediated by Ras activity. These Slit2-mediated cellular signaling processes may be involved in the proliferation and migration of mouse lung epithelial cells and are also associated with the potential mechanism of lung regeneration. Our findings suggest that Slit2 administration may be beneficial for alveolar regeneration in lungs damaged by emphysema.

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A Mouse Model for Studying Stem Cell Effects on Regeneration of Hair Follicle Outer Root Sheaths

Open Life Sciences ◽

10.1515/biol-2020-0005 ◽

2020 ◽

Vol 15 (1) ◽

pp. 41-50

Author(s):

Jingxu Guo ◽

Shuwei Li ◽

Hongyang Wang ◽

Tinghui Wu ◽

Zhenhui Wu ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Mouse Model ◽

Hair Follicle ◽

Smooth Muscle Actin ◽

Hair Follicles ◽

Alpha Smooth Muscle Actin ◽

Papillary Structure ◽

Glass Membrane

AbstractObjectiveStem cells hold promise for treating hair loss. Here an in vitro mouse model was developed using outer root sheaths (ORSs) isolated from hair follicles for studying stem cell-mediated dermal papillary regeneration.MethodsUnder sterile conditions, structurally intact ORSs were isolated from hair follicles of 3-day-old Kunming mice and incubated in growth medium. Samples were collected daily for 5 days. Stem cell distribution, proliferation, differentiation, and migration were monitored during regeneration.ResultsCell proliferation began at the glass membrane periphery then spread gradually toward the membrane center, with the presence of CD34 and CD200 positive stem cells involved in repair initiation. Next, CD34 positive stem cells migrated down the glass membrane, where some participated in ORS formation, while other CD34 cells and CD200 positive cells migrated to hair follicle centers. Within the hair follicle matrix, stem cells divided, grew, differentiated and caused outward expansion of the glass membrane to form a dermal papillary structure containing alpha-smooth muscle actin. Neutrophils attracted to the wound site phagocytosed bacterial and cell debris to protect regenerating tissue from infection.ConclusionIsolated hair follicle ORSs can regenerate new dermal papillary structures in vitro. Stem cells and neutrophils play important roles in the regeneration process.

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iTRAQ-Based Quantitative Proteomic Comparison of Early- and Late-Passage Human Dermal Papilla Cell Secretome in Relation to Inducing Hair Follicle Regeneration

PLoS ONE ◽

10.1371/journal.pone.0167474 ◽

2016 ◽

Vol 11 (12) ◽

pp. e0167474 ◽

Cited By ~ 9

Author(s):

Huan Zhang ◽

Ning-Xia Zhu ◽

Keng Huang ◽

Bo-Zhi Cai ◽

Yang Zeng ◽

...

Keyword(s):

Hair Follicle ◽

Dermal Papilla ◽

Dermal Papilla Cell ◽

Late Passage

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Airway Epithelial Cells Are Crucial Targets of Glucocorticoids in a Mouse Model of Allergic Asthma

The Journal of Immunology ◽

10.4049/jimmunol.1601691 ◽

2017 ◽

Vol 199 (1) ◽

pp. 48-61 ◽

Cited By ~ 20

Author(s):

Carina Klaßen ◽

Anna Karabinskaya ◽

Lien Dejager ◽

Sabine Vettorazzi ◽

Justine Van Moorleghem ◽

...

Keyword(s):

Epithelial Cells ◽

Mouse Model ◽

Allergic Asthma ◽

Airway Epithelial Cells ◽

Airway Epithelial

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Coordinate expression of cytokeratins 7 and 14, vimentin, and Bcl-2 in canine cutaneous epithelial tumors and cysts

Journal of Veterinary Diagnostic Investigation ◽

10.1177/1040638715594115 ◽

2015 ◽

Vol 27 (4) ◽

pp. 497-503 ◽

Cited By ~ 9

Author(s):

Jason B. Pieper ◽

Adam W. Stern ◽

Suzette M. LeClerc ◽

Karen L. Campbell

Keyword(s):

Epithelial Cells ◽

Normal Skin ◽

Basal Layer ◽

Dermal Papilla ◽

Myoepithelial Cells ◽

Vimentin Expression ◽

Outer Root Sheath ◽

Apocrine Glands ◽

Coordinate Expression ◽

Epithelial Tumors

Forty-seven canine cutaneous epithelial tumors and cysts were examined to determine coordinate expression of cytokeratins 7 (CK7) and 14 (CK14), vimentin, and Bcl-2 using commercially available antibodies. Within non-affected normal skin adjacent to tumors or cysts, CK7 expression was observed in luminal cells in apocrine glands; CK14 expression was observed in the stratum basale, stratum spinosum, stratum granulosum, basal layer of outer root sheath, sebaceous glands, and myoepithelial cells of apocrine glands; vimentin expression was observed in dermal papilla and scattered non-epithelial cells within the epidermis; and Bcl-2 expression was observed in scattered non-epithelial cells in the epidermis and some apocrine glands. The pattern of expression of CK7 and CK14 in cases of adenocarcinoma of the apocrine gland of the anal sac (CK7+/CK14–) and hepatoid gland tumors (CK7–/CK14+) may prove useful for diagnostic purposes. Loss of expression of CK14 and vimentin, identifying myoepithelial cells, was observed in apocrine and ceruminous adenocarcinomas. Differences in patterns of expression of Bcl-2 were observed between infundibular keratinizing acanthomas compared to trichoepitheliomas.

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