scholarly journals Advanced characterization of natural biofilm on nanofiber scaffold

2019 ◽  
pp. S491-S499
Author(s):  
L. Svobodová ◽  
T. Lederer ◽  
P. Rosická ◽  
P. Svoboda ◽  
L. Novák ◽  
...  
Keyword(s):  
Image Analysis ◽  
Biological Activity ◽  
Linear Correlation ◽  
Biofilm Formation ◽  
Dry Matter ◽  
Advanced Characterization ◽  
Surface Colonization ◽  
Nanofiber Scaffold ◽  
Time Required

Nanofiber scaffolds provide numerous advantages over common carriers engineered for microorganisms. The most important advantage is an increased speed of primary surface colonization (up to four times faster), which shortens the time required for the areal biofilm formation and optimum performance of attached microorganisms (higher efficiency of biological activity of up to twice as fast). Image analysis predicts early formation of biofilm even in beginning stages; analysis of biofilm reveals the different structures of bacterial colonies on both scaffolds (higher porosity, size, and number of bacterial colonies on nanofiber’s surface). The image analysis correlates well with determinations of dry matter (linear correlation of 0.96) and proteins (linear correlation of 0.89).

scholarly journals Vibrio cholerae Strains Possess Multiple Strategies for Abiotic and Biotic Surface Colonization

2007 ◽  
Vol 189 (14) ◽  
pp. 5348-5360 ◽  
Author(s):  
Ryan S. Mueller ◽  
Diane McDougald ◽  
Danielle Cusumano ◽  
Nidhi Sodhi ◽  
Staffan Kjelleberg ◽  
...  
Keyword(s):  
Vibrio Cholerae ◽  
Biofilm Formation ◽  
Acid Metabolism ◽  
Selective Advantage ◽  
Human Pathogen ◽  
Surface Colonization ◽  
Multiple Strategies ◽  
Lingulodinium Polyedrum ◽  
Transposon Mutants

ABSTRACT Despite its notoriety as a human pathogen, Vibrio cholerae is an aquatic microbe suited to live in freshwater, estuarine, and marine environments where biofilm formation may provide a selective advantage. Here we report characterization of biofilms formed on abiotic and biotic surfaces by two non-O1/O139 V. cholerae strains, TP and SIO, and by the O1 V. cholerae strain N16961 in addition to the isolation of 44 transposon mutants of SIO and TP impaired in biofilm formation. During the course of characterizing the mutants, 30 loci which have not previously been associated with V. cholerae biofilms were identified. These loci code for proteins which perform a wide variety of functions, including amino acid metabolism, ion transport, and gene regulation. Also, when the plankton colonization abilities of strains N16961, SIO, and TP were examined, each strain showed increased colonization of dead plankton compared with colonization of live plankton (the dinoflagellate Lingulodinium polyedrum and the copepod Tigriopus californicus). Surprisingly, most of the biofilm mutants were not impaired in plankton colonization. Only mutants impaired in motility or chemotaxis showed reduced colonization. These results indicate the presence of both conserved and variable genes which influence the surface colonization properties of different V. cholerae subspecies.

scholarly journals Characterization of initial events in bacterial surface colonization by twoPseudomonas species using image analysis

1992 ◽  
Vol 39 (11) ◽  
pp. 1161-1170 ◽  
Author(s):  
Robert F. Mueller ◽  
William G. Characklis ◽  
Warren L. Jones ◽  
John T. Sears
Keyword(s):  
Image Analysis ◽  
Surface Colonization ◽  
Bacterial Surface

scholarly journals Calibration of a desktop scanner and digital image analysis procedure for quantification of root morphology

2000 ◽  
Vol 9 (3) ◽  
pp. 223-230
Author(s):  
A. SIMOJOKI
Keyword(s):  
Image Analysis ◽  
Digital Image ◽  
Root Morphology ◽  
Dry Matter ◽  
Digital Image Analysis ◽  
Ethanol Solution ◽  
Analysis Procedure ◽  
High Brightness ◽  
Time Required ◽  
Metal Wires

Methodological tests with dark metal wires and barley roots showed that selecting proper scanner brightness adjustment is crucial when calibrating a desktop scanner for digital image analysis of root morphology. At high brightness the digitization of roots was incomplete, and thus the real length and width of the roots were underestimated. Lowering of the brightness improved the digitization, whereas stronger staining of the roots alone was not effective. Inaccurate results were produced also, if the size of the scanned sample was too large. The variation caused by the scanning and image analysis itself was minimal. A small amount of dry matter was lost from the roots during the preservation in the ethanol solution for several months before scanning. If absolute data on root masses are required, this loss cannot be neglected. Subsampling is strongly recommended to reduce the time required for the procedure. ;

Fabrication and Characterization of Polyoxometalate based Nano-hybrids: Evaluation of their role in Biological Activity

2019 ◽  
Vol 35 (4) ◽  
pp. 475-484
Author(s):  
SHIVA ARUN ◽  
◽  
PRABHA BHARTIYA ◽  
AMREEN NAZ ◽  
SUDHEER RAI ◽  
...  
Keyword(s):  
Biological Activity ◽  
Fabrication And Characterization
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