scholarly journals Alginate Matrices for Protein Delivery – a Short Review

2018 ◽  
pp. S319-S334 ◽  
Author(s):  
E. WAWRZYŃSKA ◽  
D. KUBIES
Keyword(s):  
Growth Factors ◽  
Regenerative Medicine ◽  
Protein Delivery ◽  
Protein Denaturation ◽  
Short Review ◽  
Primary Objective ◽  
Cellular Growth ◽  
Mechanical Stiffness ◽  
Cell Affinity ◽  
Alginate Matrices

Growth factors are powerful molecules that regulate cellular growth, proliferation, healing, and cellular differentiation. A delivery matrix that incorporates growth factors with high loading efficiencies, controls their release, and maintains bioactivity would be a powerful tool for regenerative medicine. Alginate has several unique properties that make it an excellent platform for the delivery of proteins. Mild gelling conditions can minimize the risk of protein denaturation; moreover, alginate can serve as protection from degradation until protein release. Various modifications have been proposed to tune alginate binding and release proteins, simultaneously adjusting alginate degradability, mechanical stiffness, swelling, gelation properties and cell affinity. The primary objective of this article is to review the literature related to recent advances in the application of alginate matrices in protein delivery in regenerative medicine. A special emphasis is put on the relevance of delivery of growth factors and chemokine.

scholarly journals Applications of Carbon Nanotubes in Bone Regenerative Medicine

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 659 ◽  
Author(s):  
Manabu Tanaka ◽  
Kaoru Aoki ◽  
Hisao Haniu ◽  
Takayuki Kamanaka ◽  
Takashi Takizawa ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Carbon Nanotubes ◽  
Growth Factors ◽  
Regenerative Medicine ◽  
Bone Regeneration ◽  
Chemical Properties ◽  
Clinical Applications ◽  
Review Article ◽  
Cell Affinity ◽  
Nano Size

Scaffolds are essential for bone regeneration due to their ability to maintain a sustained release of growth factors and to provide a place where cells that form new bone can enter and proliferate. In recent years, scaffolds made of various materials have been developed and evaluated. Functionally effective scaffolds require excellent cell affinity, chemical properties, mechanical properties, and safety. Carbon nanotubes (CNTs) are fibrous nanoparticles with a nano-size diameter and have excellent strength and chemical stability. In the industrial field, they are used as fillers to improve the performance of materials. Because of their excellent physicochemical properties, CNTs are studied for their promising clinical applications as biomaterials. In this review article, we focused on the results of our research on CNT scaffolds for bone regeneration, introduced the promising properties of scaffolds for bone regeneration, and described the potential of CNT scaffolds.

scholarly journals Growth Factors in Regeneration and Regenerative Medicine: “the Cure and the Cause”

2020 ◽  
Vol 11 ◽  
Author(s):  
Konstantin Yu. Kulebyakin ◽  
Peter P. Nimiritsky ◽  
Pavel I. Makarevich
Keyword(s):  
Growth Factors ◽  
Regenerative Medicine

Useful applications of growth factors for cardiovascular regenerative medicine

2020 ◽  
Vol 38 (1) ◽  
pp. 35-63
Author(s):  
Maria Rosaria De Pascale ◽  
Nunzia Della Mura ◽  
Michele Vacca ◽  
Claudio Napoli
Keyword(s):  
Growth Factors ◽  
Regenerative Medicine

scholarly journals The Role of Steroids, Growth Factors and CAMP Stimulators on the Gap Junction Activity in Cumulus Oocyte Complexes in the Rat

2021 ◽  
Author(s):  
◽  
Manvi Yadav
Keyword(s):  
Growth Factors ◽  
Gap Junction ◽  
Cumulus Cells ◽  
Antral Follicle ◽  
Relative Increase ◽  
Primary Objective ◽  
Dye Transfer ◽  
Oocyte Growth ◽  
Phosphodiesterase Type

<p>Bidirectional communication between mammalian oocytes and their surrounding somatic cells is essential for oocyte maturation. Gap junctions promote the transfer of essential metabolites, nucleotides, amino acids and ions from cumulus cells to the oocyte that are crucial for oocyte growth and development. However, the range of factors present in the microenvironment of the developing antral follicle, which modulate gap junction activity of the cumulus-oocyte complexes (COCs), is largely unknown. The primary objective of this study was to determine the effects of various steroids, growth factors and cAMP stimulators on the gap junction activity in rat COCs. The gap junction activity was measured in presence or absence of different treatments using a fluorescence dye and in the presence of milrinone, a phosphodiesterase type 3 inhibitor. The major findings of this study were that cAMP stimulators increased the rate of dye transfer from cumulus cells to the oocyte. Under in vitro conditions it was established that neither steroids nor IGF1 by themselves were able to modulate gap junction activity in rat COCs. Furthermore, forskolin, a potent cAMP stimulator; caused a relative increase in Cx37 gene expression levels following a four hours incubation period. The outcomes from the present study may help to provide new insights into developing suitable in vitro conditions, for the in vitro maturation of mammalian oocytes.</p>

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