scholarly journals MCT1 and MCT4 Kinetic of mRNA Expression in Different Tissues After Aerobic Exercise at Maximal Lactate Steady State Workload

2015 ◽  
pp. 513-522 ◽  
Author(s):  
G. G. DE ARAUJO ◽  
C. A. GOBATTO ◽  
F. DE BARROS MANCHADO-GOBATTO ◽  
L. F. M. TEIXEIRA ◽  
I. G. M. DOS REIS ◽  
...  
Keyword(s):  
Steady State ◽  
Mrna Expression ◽  
Acute Exercise ◽  
Swimming Exercise ◽  
Exercise Session ◽  
Control Group ◽  
Maximal Lactate Steady State ◽  
Single Bout ◽  
White Gastrocnemius

We evaluate the mRNA expression of monocarboxylate transporters 1 and 4 (MCT1 and MCT4) in skeletal muscle (soleus, red and white gastrocnemius), heart and liver tissues in mice submitted to a single bout of swimming exercise at the maximal lactate steady state workload (MLSSw). After 72 h of MLSS test, the animals were submitted to a swimming exercise session for 25 min at individual MLSSw. Tissues and muscle samples were obtained at rest (control, n=5), immediately (n=5), 5 h (n=5) and 10 h (n=5) after exercise for determination of the MCT1 and MCT4 mRNA expression (RT-PCR). The MCT1 mRNA expression in liver increased after 10 h in relation to the control, immediate and 5 h groups, but the MCT4 remained unchanged. The MCT1 mRNA expression in heart increased by 31 % after 10 h when compared to immediate, but no differences were observed in relation to the control group. No significant differences were observed for red gastrocnemius in MCT1 and MCT4 mRNA expression. However, white gastrocnemius increased MCT1 mRNA expression immediately when compared to rest, 5 and 10 h test groups. In soleus muscle, the MCT1 mRNA expression increased immediately, 5 and 10 h after exercise when compared to the control. In relation to MCT4 mRNA expression, the soleus increased immediately and 10 h after acute exercise when compared to the control group. The soleus, liver and heart were the main tissues that showed improved the MCT1 mRNA expression, indicating its important role in controlling MLSS concentration in mice.

Mood Alterations in Older Adults following Acute Exercise

1994 ◽  
Vol 79 (1) ◽  
pp. 191-194 ◽  
Author(s):  
Edgar F. Pierce ◽  
Donald W. Pate
Keyword(s):  
Older Adults ◽  
Repeated Measures ◽  
Acute Exercise ◽  
Mood States ◽  
Exercise Session ◽  
Control Group ◽  
Older Individuals ◽  
Significant Difference ◽  
Single Bout ◽  
Affective Change

Limited research indicates positive affective change following acute bouts of exercise, but whether this improved affect among younger subjects may be generalized to older individuals is not known. The present study, then, examined the effects of a single bout of physical activity among older participants. 16 trained women ( Moge = 64.5 ± 7.6 yr.) completed an abbreviated Profile of Mood States prior to and immediately following a 75-min. session of aerobic line dancing. A series of one-way analyses of variance with repeated measures were used to examine differences between pre- and posttest subscores on mood states. Significant decreases following exercise in scores on Tension, Depression, Fatigue, and Anger and a significant increase in scores on Vigor relative to preexercise (control) scores were found. Global mood was significantly improved after the exercise session. No significant difference was found between pre- and postexercise measures of Confusion. Previous findings of significant improvements in affect immediately after an acute bout of exercise may be generalized to older adults. Repetition with a nonexercised control group is desirable.

Determination of the Maximal Lactate Steady State in Obese Zucker Rats

2012 ◽  
Vol 34 (03) ◽  
pp. 214-217 ◽  
Author(s):  
J. Almeida ◽  
B. Petriz ◽  
C.P. Gomes ◽  
L.A. Rocha ◽  
R. Pereira ◽  
...  
Keyword(s):  
Steady State ◽  
Zucker Rats ◽  
Maximal Lactate Steady State ◽  
Obese Zucker Rats

scholarly journals The Effect of Single Bout of Acute Exercise on Working Memory Performance

2018 ◽  
Vol 3 (2) ◽  
pp. 484-487
Author(s):  
Santosh Kumar Deo ◽  
Kopila Agrawal ◽  
Prem Bhattrai ◽  
Raju Kumar Chaudhary
Keyword(s):  
Decision Making ◽  
Working Memory ◽  
Acute Exercise ◽  
Test Procedure ◽  
Step Test ◽  
Moderate Intensity ◽  
Exercise Session ◽  
Moderate Intensity Exercise ◽  
Single Bout ◽  
Back Task

Introduction: Working memory is a kind of short term memory important for reasoning and guiding decision-making and behavioral process.Objective: The goal of the present research was to study the outcome of single bout of acute moderate-intensity exercise on working memory.Methodology: Twenty two male subjects were asked to perform working memory task by 2n back task in baseline resting, immediately after exercise and after five minute of exercise session. 3 minute step test procedure was used as a moderate intensity exercise intervention.Results: The percentage correctness of 2n back task of working memory was found to be 64.36% for baseline resting condition, 78.01 % for immediately after 3-minute step test and 80.70% for 5 minute after the exercise. In both exercise session (i.e. immediately after exercise and after 5 minute of exercise), significant improvement (p value <0.05) in working memory was seen as compared to the baseline resting session while no such significant beneficial improvement was seen when compared between immediately after exercise and after 5 minute of exercise.Conclusion: Improvement in working memory after moderate exercise intervention was seen, which is important for learning and memory and decision-making.  BJHS 2018;3(2)6:484-487.

Rapid Determination of Maintenance Heparin Infusion Rates with the Use of Non-Steady-State Heparin Concentrations

1993 ◽  
Vol 27 (12) ◽  
pp. 1429-1433 ◽  
Author(s):  
Robert J. Kandrotas ◽  
Peter Gal ◽  
Jean B. Douglas ◽  
James B. Groce
Keyword(s):  
Steady State ◽  
Venous Thrombosis ◽  
Deep Venous Thrombosis ◽  
Rapid Determination ◽  
Heparin Therapy ◽  
Control Group ◽  
Heparin Infusion ◽  
The Difference ◽  
Dosing Method

OBJECTIVE: To compare heparin dosage adjustment using only activated partial thromboplastin time (APTT) with a method using non-steady-state heparin concentrations (HCs) to rapidly achieve and maintain an APTT ratio greater than or equal to 1.5 times baseline throughout the first 24 hours of therapy. DESIGN: Randomized, blind, parallel comparison of an empiric dosing method based only on APTT with a dosing method based on the calculation of heparin clearance using non-steady-state HCs. SETTING: A private community teaching hospital. The patient, physician, nurses, and investigators were blinded to the dosing method. Only the clinical staff pharmacist, who received the consult and made all dosage adjustments, was not blinded. PATIENTS: All patients requiring heparin for the treatment of thromboembolic disease were evaluated for potential inclusion in the study. Patients were enrolled in the study if they had a clinical diagnosis of deep venous thrombosis confirmed by objective means such as venography or ultrasonography. Patients were excluded if they had active bleeding, platelet dysfunction, thrombocytopenia, severe hepatic disease (total bilirubin >25.7 μmol/L), renal disease, or evidence of stroke. Patients were also excluded if they were receiving heparin prior to enrollment. MAIN OUTCOME MEASURE: Maintenance of an APTT ratio greater than or equal to 1.5 times baseline throughout the first 24 hours of heparin therapy. RESULTS: Thirty-four patients were enrolled in the study; 17 in each group. The groups were not significantly different with regard to gender, age, baseline APTT, or mean loading dose (p>0.5). Mean initial infusion rates for the control and HC groups were 1042 ± 194 and 1071 ± 143 units/h, respectively (p>0.5). After the first rate adjustment at 4 hours, the difference achieved significance at 1032 ± 232 and 1367 ± 317 units/h for the control and HC groups, respectively (p<0.01). At 12 hours, 18.8 percent of the patients in the control group were subtherapeutic; by 24 hours, 33.3 percent were subtherapeutic. No patients became subtherapeutic in the HCs group during the first 24 hours. CONCLUSIONS: This study demonstrates that, in contrast to standard heparin dosing methods, the use of non-steady-state HCs allows patients with deep venous thrombosis to rapidly achieve and maintain therapeutic APTT ratios throughout the critical first 24 hours of therapy.

DETERMINATION OF MAXIMAL LACTATE STEADY STATE VELOCITY : COINCIDENCE WITH LOWER BLOOD GLUCOSE 405

1996 ◽  
Vol 28 (Supplement) ◽  
pp. 68 ◽  
Author(s):  
H. G. Sim??es ◽  
C. S.G. Campbell ◽  
E. Kokubun ◽  
B. S. Denadai ◽  
V. Baldissera
Keyword(s):  
Steady State ◽  
Blood Glucose ◽  
Maximal Lactate Steady State ◽  
Lower Blood Glucose ◽  
Lower Blood

Specific Determination of Maximal Lactate Steady State in Soccer Players

2015 ◽  
Vol 29 (1) ◽  
pp. 101-106 ◽  
Author(s):  
João P. Loures ◽  
Karim Chamari ◽  
Eliel C. Ferreira ◽  
Eduardo Z. Campos ◽  
Alessandro M. Zagatto ◽  
...  
Keyword(s):  
Steady State ◽  
Soccer Players ◽  
Maximal Lactate Steady State ◽  
Specific Determination

Biomarkers of oxidative stress and tissue damage released by muscle and liver after a single bout of swimming exercise

2013 ◽  
Vol 38 (5) ◽  
pp. 507-511 ◽  
Author(s):  
Dionizio Ramos ◽  
Eduarda Gabrielle Martins ◽  
Diego Viana-Gomes ◽  
Gustavo Casimiro-Lopes ◽  
Verônica P. Salerno
Keyword(s):  
Oxidative Stress ◽  
Acute Exercise ◽  
Cellular Damage ◽  
Swimming Exercise ◽  
Antioxidant Systems ◽  
Inadequate Response ◽  
Oxidative Stress Biomarkers ◽  
Stress Biomarkers ◽  
Single Bout ◽  
Biomarkers Of Oxidative Stress

Both acute exercise and excessive training can cause oxidative stress. The resulting increase in free radicals and the inadequate response from antioxidant systems can lead to a framework of cellular damage. An association between affected tissue and the biomarkers of oxidative stress that appear in plasma has not been clearly established. The aim of this study was to evaluate the source of oxidative stress biomarkers found in the plasma of untrained rats after a single bout of swimming exercise at 2 different intensities: low intensity (SBLIE) or high intensity (SBHIE). Immediately after the exercise, aspartate transaminase (AST), alanine transaminase (ALT), γ-glutamyltransferase (GGT), and lactate dehydrogenase (LDH) were measured in plasma to characterize cell damage. Oxidative stress was assessed using protein carbonylation (PC), total antioxidant capacity (TAC), and thiobarbituric acid reactive substances (TBARS) quantified by malondialdehyde concentration. SBHIE raised levels of plasma AST (93%) and ALT (17%), and both exercise regimens produced an increase in GGT (7%) and LDH (∼55%). Plasma levels of PC and TBARS were greater in the SBHIE group; there were no changes in TAC. SBLIE caused only a modest increase in TBARS. In muscle, there were no changes in TAC, PC, or TBARS, regardless of exercise intensity, In the liver, TAC and TBARS increased significantly in both the SBLIE and SBHIE groups. This indicates that the oxidative stress biomarkers measured in the plasma immediately after a single bout of swimming exercise were generated primarily in the liver, not in muscle.

Maximal lactate steady state in rats submitted to swimming exercise

2001 ◽  
Vol 130 (1) ◽  
pp. 21-27 ◽  
Author(s):  
Claudio Alexandre Gobatto ◽  
Maria Alice Rostom de Mello ◽  
Clarice Yoshiko Sibuya ◽  
José Roberto Moreira de Azevedo ◽  
Luciana Alves dos Santos ◽  
...  
Keyword(s):  
Steady State ◽  
Swimming Exercise ◽  
Maximal Lactate Steady State
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