scholarly journals Anti-Helicobacter Pylori, Anti-Thyroid Peroxidase, Anti-Thyroglobulin and Anti-Gastric Parietal Cells Antibodies in Czech Population

2008 ◽  
pp. S135-S141
Author(s):  
I Šterzl ◽  
P Hrdá ◽  
P Matucha ◽  
J Čeřovská ◽  
V Zamrazil
Keyword(s):  
Helicobacter Pylori ◽  
Thyroid Autoimmunity ◽  
Parietal Cells ◽  
Thyroid Peroxidase ◽  
Gastric Parietal Cell ◽  
Czech Population ◽  
Study Support ◽  
H Pylori ◽  
Gastric Parietal Cells ◽  
Age And Sex

Autoimmune thyropathies are frequently linked to many infections, such as Helicobacter pylori, which are also supposed to play a role in their pathogenesis. The aim of this study was to evaluate the relationships between thyroid and gastric autoimmunity and H. pylori infection on a large sample of Czech population (n=1621) by monitoring the autoantibodies against thyroglobulin (anti-Tg) and thyroid peroxidase (anti-TPO) and gastric parietal cell (anti-GPC, representing thyrogastric syndrome) in correlation with antibodies against Helicobacter pylori (anti-H. pylori) of classes IgG and IgA. The interrelation between autoantibodies and H. pylori antibodies was assessed by H. pylori seropositivity. In H. pylori seropositive persons as compared to seronegative irrespective of age and sex, a higher occurrence of anti-TPO (10.4 % vs. 5.8 %, p=0.001) and anti-GPC (6.1 % vs. 1.7 %, p<0.001) was found. Differences in anti-TPO occurrence were significant in both men (7.0 % vs. 3.3 %, p=0.03) and women (12.7 % vs. 8.0 %, p=0.02), differences in anti-GPC occurrence were significant only in women (7.2 % vs. 1.7 %, p<0.001). Results of this study support the idea of a connection between infection of H. pylori and the occurrence of anti-TPO autoantibodies representing thyroid autoimmunity and gastric parietal cells autoantibodies representing the thyrogastric syndrome.

scholarly journals CHAC1 overexpression in human gastric parietal cells with Helicobacter pylori infection in the secretory canaliculi

Helicobacter ◽  
2019 ◽  
pp. e12598
Author(s):  
Tomohisa Ogawa ◽  
Yuriko Wada ◽  
Kosuke Takemura ◽  
Philip G. Board ◽  
Keisuke Uchida ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Parietal Cells ◽  
Helicobacter Pylori Infection ◽  
Gastric Parietal Cells

scholarly journals Induction of Follicular Gastritis following Postthymectomy Autoimmune Gastritis in Helicobacter pylori-Infected BALB/c Mice

2000 ◽  
Vol 68 (1) ◽  
pp. 100-106 ◽  
Author(s):  
Chikashi Oshima ◽  
Kazuichi Okazaki ◽  
Yumi Matsushima ◽  
Mitsutaka Sawada ◽  
Tsutomu Chiba ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Early Stage ◽  
Parietal Cells ◽  
Antibody Responses ◽  
Autoimmune Gastritis ◽  
Interleukin 4 ◽  
Fundic Gland ◽  
H Pylori ◽  
Gland Atrophy ◽  
Follicular Gastritis

ABSTRACT Helicobacter pylori is the major causative agent of chronic antral gastritis and is thought to be involved in the pathogenesis of mucosa-associated lymphoid tissue lymphoma (MALToma) developing in the human stomach. The aim of this study was to clarify whether corporal autoimmune gastritis (AIG), which is known to decrease acidity due to destruction of parietal cells, predisposes mice toH. pylori infection, thereby leading to MALToma-like pathology. BALB/c mice in which AIG had been induced by thymectomy 3 days after birth (AIG mice) were used. The AIG mice were orally administered mouse-adapted H. pylori at the age of 6 weeks and were examined histologically and serologically after 2 to 12 months. The results were compared with those obtained from uninfected AIG mice and infected normal mice. Germinal centers were induced in the corpus in 57% of the H. pylori-infected AIG mice, which elicited anti-H. pylori antibody responses in association with upregulation of interleukin-4 (IL-4) mRNA. In these mice, parietal cells remained in the corpus mucosa. These findings were in contrast to those with the uninfected AIG mice: fundic gland atrophy due to disappearance of parietal cells associated with upregulation of gamma interferon, but not IL-4, mRNA and no germinal center formation in the corpus. These observations suggest that AIG alters the infectivity ofH. pylori, leading to MALToma-like follicular gastritis, at an early stage after H. pylori infection.

Effect of Pistacia atlantica on the elimination of Helicobacter pylori and improvement of gastric reflux in patients with chronic cough: A randomized clinical trial

2021 ◽  
Vol 07 ◽  
Author(s):  
Masoud Shirmohammadi ◽  
Milad Asadi ◽  
Venus Zafari ◽  
Yeganeh Khalili ◽  
Habib Zarredar ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Helicobacter Pylori ◽  
Chronic Cough ◽  
Intervention Group ◽  
Gastrointestinal Complications ◽  
Pistacia Atlantica ◽  
Stool Samples ◽  
Gastric Reflux ◽  
H Pylori ◽  
Age And Sex

Background: Pistacia atlantica (P. atlantica) has been associated with beneficial medical effects on gastrointestinal complications. Objective: It was intended to assess the effects of P. atlantica in elimination of Helicobacter pylori (H. pylori) infection and amelioration of gastric reflux and chronic cough. Methods: In this clinical trial study, 100 subjects with H. pylori infection who had the symptoms of gastric reflux and chronic cough were recruited and classified into two intervention and placebo groups, continuing 50 subjects each. The intervention group was administered two 500 mg doses of P. atlantica extract each day. The H. pylori antigen load in stool samples was evaluated using ELISA. The severity of cough and gastric re flux was also determined. Results: The subjects in two groups were age and sex matched and the baseline measurements were similar on the day 0. Nonetheless, it was observed that on the day 14, there was a significant reduction of H. pylori antigen load in the intervention group compared with the placebo group (P = 0.00021). Furthermore, the intensity of chronic cough was reduced significantly in the intervention group (P = 0.0014). The gastric reflux was improved in the intervention group at the end of the study. Conclusion: P. atlantica extract was beneficial in controlling H. pylori infection. It also improved the complications of the infection, like gastric reflux and chronic cough. Hence, it is highly recommended to value natural products, along with traditional antibiotics, in ameliorating H. pylori infection.

scholarly journals Seroepidemiology ofHelicobacter pyloriinfection in vegans and meat-eaters

1992 ◽  
Vol 108 (3) ◽  
pp. 457-462 ◽  
Author(s):  
M. J. Webberley ◽  
J. M. Webberley ◽  
D. G. Newell ◽  
P. Lowe ◽  
V. Melikian
Keyword(s):  
Helicobacter Pylori ◽  
Risk Factor ◽  
Campylobacter Jejuni ◽  
Meat Consumption ◽  
Cross Reactivity ◽  
Enzyme Linked Immunosorbent Assay ◽  
Igg Antibody ◽  
Specific Igg ◽  
H Pylori ◽  
Age And Sex

SUMMARYAn enzyme-linked immunosorbent assay has been used to diagnose serologically the prevalence ofHelicobacter pyloriinfection in Asian life-long vegans. There was no difference in the seropositivity between these individuals and a group of age-and sex-matched Asian meat-eaters, indicating the meat consumption is not a risk factor forH. pyloriinfection. However, both Asian groups had a higher prevalence of infection than age- and sex-matched Caucasian meat-eaters. Additionally, the Asian individuals had a wider range of specific IgG antibody concentrations than the Caucasians. This did not appear to be due to antigenic cross-reactivity betweenH. pyloriandCampylobacter jejuni. The significance of these observations to the establishment of cut-off levels for the serodiagnosis of certain ethnic groups is discussed.

scholarly journals Identification and characterization of a novel A-Kinase-anchoring protein (AKAP120) from rabbit gastric parietal cells

1997 ◽  
Vol 322 (3) ◽  
pp. 801-808 ◽  
Author(s):  
Daniel T. DRANSFIELD ◽  
Jennifer L. YEH ◽  
Alvin J. BRADFORD ◽  
James R. GOLDENRING
Keyword(s):  
Amino Acids ◽  
Gastric Fundus ◽  
Parietal Cells ◽  
Regulatory Subunit ◽  
Binding Region ◽  
Rnase Protection ◽  
Gastric Parietal Cell ◽  
High Affinity ◽  
Gastric Parietal Cells ◽  
Recombinant Fragments

The type-II cAMP-dependent protein kinase (A-Kinase) partitions primarily into the particulate fraction in gastric parietal cells. Localization of this kinase to particular subcellular domains is mediated through the binding of the regulatory subunit (RII) dimer to A-Kinase-anchoring proteins (AKAPs). Using a [32P]RII overlay assay, we have screened a rabbit gastric parietal cell cDNA library and have isolated a single RII-binding protein clone. Sequence analysis revealed an open reading frame coding for 1022 amino acids (AKAP120). Recombinant fragments of the full-length clone were prepared and the RII-binding region mapped to an area between amino acids 489 and 549. This area contained a putative α-helical RII-binding region between amino acids 503 and 516. Incubation of [32P]RII with a synthetic peptide of AKAP120-(489–522) completely inhibited the binding of [32P]RII to the recombinant AKAP120 fragments that demonstrated RII binding. In vitroRII -binding affinity studies indicated a high-affinity interaction between AKAP120 and RII with a Kapp between 50 and 120 nM for the three recombinant fragments that bound [32P]RII. RNase-protection analysis revealed that AKAP120 is a widely distributed protein, with the highest levels of mRNA observed in gastric fundus. The presence of this novel high-affinity AKAP in gastric parietal cells suggests that it may regulate RII subcellular sequestration in this cell type.

Gastric parietal cell H(+)-K(+)-ATPase microsomes are associated with isoforms of ankyrin and spectrin

1993 ◽  
Vol 264 (1) ◽  
pp. C63-C70 ◽  
Author(s):  
P. R. Smith ◽  
A. L. Bradford ◽  
E. H. Joe ◽  
K. J. Angelides ◽  
D. J. Benos ◽  
...  
Keyword(s):  
Apical Membrane ◽  
Immunoblot Analysis ◽  
Cytoskeletal Proteins ◽  
Parietal Cells ◽  
Apical Surface ◽  
Gastric Parietal Cell ◽  
Apical Domain ◽  
Hcl Secretion ◽  
Gastric Parietal Cells ◽  
Stimulation Of

Stimulation of HCl secretion by gastric parietal cells requires the fusion of cytoplasmic H(+)-K(+)-ATPase-bearing tubulovesicles with the apical membrane. This insertion of membrane results in a dramatic increase in apical surface area through the formation of microvilli. To elucidate the elements that may stabilize the newly inserted H(+)-K(+)-ATPase within the apical membrane, we searched for specific cytoskeletal proteins associating with the gastric enzyme. We document by immunoblot analysis that ankyrin, spectrin, and actin copurify with H(+)-K(+)-ATPase microsomes prepared from gastric parietal cells. Coprecipitation of 125I-labeled native erythrocyte ankyrin with the H(+)-K(+)-ATPase from gastric microsomes using anti-H(+)-K(+)-ATPase antibodies suggests that ankyrin associates with the H(+)-K(+)-ATPase. Indirect immunofluorescence and confocal microscopy show that ankyrin and H(+)-K(+)-ATPase cosegregate within resting and secreting parietal cells. Taken together, these data suggest that the association of the gastric H(+)-K(+)-ATPase with spectrin and actin is mediated by ankyrin and that this interaction contributes to the maintenance of the polarized distribution of the enzyme to the apical domain of gastric parietal cells during acid secretion.

Helicobacter pyloriinduces apoptosis of rat gastric parietal cells

2002 ◽  
Vol 283 (2) ◽  
pp. G309-G318 ◽  
Author(s):  
Bruno Neu ◽  
Pamela Randlkofer ◽  
Mathilde Neuhofer ◽  
Petra Voland ◽  
Artur Mayerhofer ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Parietal Cells ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Dependent Manner ◽  
Knockout Mutant ◽  
Recognition Sequence ◽  
Mobility Shift ◽  
Nuclear Extracts ◽  
H Pylori ◽  
Gastric Parietal Cells

Gastric Helicobacter pylori infection may lead to multifocal atrophic corpus gastritis associated with loss of epithelial cells as well as glandular structures. The current work investigated H. pylori effects on cell death of isolated, nontransformed rat parietal cells (PC). Highly enriched rat PC (>97%) were isolated from gastric mucosa and cultured in serum-free medium over 24 h. The cells were cocultured over 8 h with cytotoxin-associated immunodominant protein (cagA)+/vacuolating toxin (vacA)+or with cagA−/vacA−H. pylori laboratory strains and also with H. pylori mutants deleted in several genes of the cag pathogenicity island. Staphylococcus aureus or Campylobacter jejuni were used as controls. Apoptosis was determined by terminal deoxynucleotidyl transferase dUTP nick-end labeling staining and electron microscopy. Interleukin (IL)-8 and cytokine-induced neutrophil chemoattractant (CINC)-1 secretion was measured by ELISA. Activation of nuclear factor-κB (NF-κB) was studied in nuclear extracts of PC by electrophoretic mobility shift assay. Apoptosis of PC was induced in a concentration- and time-dependent manner by cagA+/vacA+H. pylori strains but not by cagA−/vacA−negative strains or by the cagE knockout mutant. S. aureusand C. jejuni had no effect. PC showed no IL-8 or CINC-1 secretion on exposure to cagA+/vacA+H. pylori. cagA+/vacA+strains induced activation of NF-κB complexes in nuclear extracts of PC, which were composed of p65 and p50 subunits. No significant stimulation of NF-κB activation was detected by incubation of PC with the cagE knockout mutant. Preincubation of PC with antisense but not missense oligodeoxynucleotides against the p65 subunit significantly reduced DNA binding to the κB recognition sequence. The p65 oligonucleotides as well as the proteasome inhibitor N-CBZ-isoleucin-glutamin-(o-t-butyl-)-alanin-leucin and the nitric oxide synthase inhibitor NG-monomethyl-l-arginine completely prevented PC apoptosis induced by cagA+/vacA+strains. In summary, cagE presence appears to be essential for H. pylori-induced apoptosis of gastric parietal cells, and this effect is dependent on the activation of NF-κB and production of nitric oxide.

scholarly journals Seroprevalence of Helicobacter pylori infection in chagasic and nonchagasic patients from the same geographical region of Brazil

2012 ◽  
Vol 45 (2) ◽  
pp. 194-198 ◽  
Author(s):  
Fernanda Machado Fonseca ◽  
Dulciene Maria de Magalhães Queiroz ◽  
Andreia Maria Camargos Rocha ◽  
Aluízio Prata ◽  
Eduardo Crema ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Multivariate Analysis ◽  
Odds Ratio ◽  
Helicobacter Pylori Infection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Clinical Form ◽  
Serological Tests ◽  
Clinical Forms ◽  
H Pylori ◽  
Age And Sex

INTRODUCTION: In this study, we evaluated the seroprevalence of Helicobacter pylori infection among chagasic and non-chagasic subjects as well as among the subgroups of chagasic patients with the indeterminate, cardiac, digestive, and cardiodigestive clinical forms. METHODS: The evaluated subjects were from the Triângulo Mineiro region, Minas Gerais, Brazil. Chagasic patients showed positive reactions to the conventional serological tests used and were classified according to the clinical form of their disease. Immunoglobulin G antibodies specific to H. pylori were measured using a commercial enzyme-linked immunosorbent assay kit. RESULTS: The overall H. pylori prevalence was 77.1% (239/310) in chagasic and 69.1% (168/243) in non-chagasic patients. This difference was statistically significant even after adjustment for age and sex (odds ratio = 1.57; 95% confidence interval, 1.02-2.42; p = 0.04) in multivariate analysis. The prevalence of infection increased with age in the non-chagasic group (p = 0.007, χ2 for trend), but not in the chagasic group (p = 0.15, χ2 for trend). H. pylori infection was not associated with digestive or other clinical forms of Chagas disease (p = 0.27). CONCLUSIONS: Our findings demonstrate that chagasic patients have a higher prevalence of H. pylori compared to non-chagasic subjects; a similar prevalence was found among the diverse clinical forms of the disease. The factors contributing to the frequent co-infection with H. pylori and Trypanosoma cruzi as well as its effects on the clinical outcome deserve further study.

scholarly journals Effects of Helicobacter pylori Infection on Gastric Parietal Cells and E-cadherin in Mongolian Gerbils

2013 ◽  
Vol 121 (4) ◽  
pp. 305-311 ◽  
Author(s):  
Motonobu Murakami ◽  
Mayu Fukuzawa ◽  
Mika Yamamoto ◽  
Kanako Hamaya ◽  
Yuumi Tamura ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Parietal Cells ◽  
Helicobacter Pylori Infection ◽  
Mongolian Gerbils ◽  
Gastric Parietal Cells ◽  
E Cadherin
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