scholarly journals Exploring the Links Between Oxidative Stress, RNA Damage and Disease

2021 ◽  
Author(s):  
Marino Resendiz
Keyword(s):  
Oxidative Stress ◽  
Rna Damage

Markers of HPA-axis activity and nucleic acid damage from oxidation after electroconvulsive stimulations in rats

2019 ◽  
Vol 31 (6) ◽  
pp. 287-293
Author(s):  
Anders Jorgensen ◽  
Katrine Breitenstein ◽  
Otto Kalliokoski ◽  
Allan Weimann ◽  
Trine Henriksen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Mrna Expression ◽  
Hpa Axis ◽  
Severe Depression ◽  
Male Rats ◽  
Therapeutic Effects ◽  
Middle Aged ◽  
Dna And Rna ◽  
Rna Damage ◽  
Hpa Axis Activity

AbstractObjective:Oxidative stress has been suggested to increase after electroconvulsive therapy (ECT), a treatment which continues to be the most effective for severe depression. Oxidative stress could potentially be mechanistically involved in both the therapeutic effects and side effects of ECT.Methods:We measured sensitive markers of systemic and central nervous system (CNS) oxidative stress on DNA and RNA (urinary 8-oxodG/8-oxoGuo, cerebrospinal fluid 8-oxoGuo, and brain oxoguanine glycosylase mRNA expression) in male rats subjected to electroconvulsive stimulations (ECS), an animal model of ECT. Due to the previous observations that link hypothalamic–pituitary–adrenal (HPA)-axis activity and age to DNA/RNA damage from oxidation, groups of young and middle-aged male animals were included, and markers of HPA-axis activity were measured.Results:ECS induced weight loss, increased corticosterone (only in middle-aged animals), and decreased cerebral glucocorticoid receptor mRNA expression, while largely leaving the markers of systemic and CNS DNA/RNA damage from oxidation unaltered.Conclusion:These results suggest that ECS is not associated with any lasting effects on oxidative stress on nucleic acids neither in young nor middle-aged rats.

scholarly journals RNA damage and degradation under oxidative stress

2009 ◽  
Vol 23 (S1) ◽  
Author(s):  
Min Liu ◽  
Zhongwei Li
Keyword(s):  
Oxidative Stress ◽  
Rna Damage

scholarly journals Distinct pattern of oxidative DNA damage and DNA repair in follicular thyroid tumours

2012 ◽  
Vol 48 (3) ◽  
pp. 193-202 ◽  
Author(s):  
Stefan Karger ◽  
Kerstin Krause ◽  
Cornelia Engelhardt ◽  
Carl Weidinger ◽  
Oliver Gimm ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Damage ◽  
Dna Repair ◽  
Oxidative Dna Damage ◽  
Follicular Adenoma ◽  
Distinct Pattern ◽  
Proliferation Index ◽  
Checkpoint Activation ◽  
Rna Damage

Increased oxidative stress has been linked to thyroid carcinogenesis. In this paper, we investigate whether oxidative DNA damage and DNA repair differ in follicular adenoma (FA) and follicular thyroid carcinoma (FTC). 7,8-Dihydro-8-oxoguanine (8-OxoG) formation was analysed by immunohistochemistry in 46 FAs, 52 FTCs and 18 normal thyroid tissues (NTs). mRNA expression of DNA repair genes OGG1, Mut Y homologue (MUTYH) and endonuclease III (NTHL1) was analysed by real-time PCR in 19 FAs, 25 FTCs and 19 NTs. Induction and repair of oxidative DNA damage were studied in rat FRTL-5 cells after u.v. irradiation. Moreover, activation of DNA damage checkpoints (ataxia telangiectasia mutated (ATM) and H2A histone family, member X (H2AFX (H2AFX))) and proliferation index (MIB-1) were quantified in 28 non-oxyphilic and 24 oxyphilic FTCs. Increased nuclear and cytosolic 8-OxoG formation was detected in FTC compared with follicular adenoma, whereby cytosolic 8-OxoG formation was found to reflect RNA oxidation. Significant downregulation of DNA repair enzymes was detected in FTC compared with FA. In vitro experiments mirrored the findings in FTC with oxidative stress-induced DNA checkpoint activation and downregulation of OGG1, MUTYH and NTHL1 in FRTL-5 cells, an effect that, however, was reversible after 24 h. Further analysis of FTC variants showed decreased oxidative DNA damage, sustained checkpoint activation and decreased proliferation in oxyphilic vs non-oxyphilic FTC. Our data suggest a pathophysiological scenario of accumulating unrepaired DNA/RNA damage in FTC vs counterbalanced DNA/RNA damage and repair in FA. Furthermore, this study provides the first evidence for differences in oxidative stress defence in FTC variants with possible implications for therapeutic response and prognostic outcome.

scholarly journals Cerebrospinal fluid oxidative stress metabolites in patients with bipolar disorder and healthy controls: a longitudinal case-control study

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Ulla Knorr ◽  
Anja Hviid Simonsen ◽  
Peter Roos ◽  
Allan Weimann ◽  
Trine Henriksen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Bipolar Disorder ◽  
Cerebrospinal Fluid ◽  
Case Control Study ◽  
Case Control ◽  
Oxidative Stress Markers ◽  
Stress Markers ◽  
Rna Damage ◽  
Control Study

AbstractBipolar disorder (BD) is a mental disorder characterized by recurrent relapses of affective episodes, cognitive impairment, illness progression, and reduced life expectancy. Increased systemic oxidatively generated nucleoside damage have been found in some neurodegenerative disorders and in BD. As the first, this naturalistic prospective, longitudinal follow-up case-control study investigated cerebrospinal fluid (CSF) oxidative stress markers 8-oxo-7,8-dihydroguanosine (8-oxoGuo) and 8-oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodG) that relate to RNA and DNA damage, respectively. Patients with BD (n = 86, 51% female) and gender-and-age-matched healthy control individuals (HC; n = 44, 44% female) were evaluated at baseline (T0), during (T1) and after a new affective episode (T2), if it occurred, and after a year (T3). Cerebrospinal and urine oxidative stress markers were analyzed using ultra-performance liquid chromatography–tandem mass spectrometry. CSF-8-oxoGuo was statistically significantly higher by 18% (p = 0.003) in BD versus HC at T0, and by 22% (p = 0) at T3. CSF-8-oxoGuo had increased by 15% (p = 0.042) from T0 to T3, and by 14% (p = 0.021) from T2 to T3 in patients, who experienced an episode during follow-up. CSF-8-oxodG had increased by 26% (p = 0.054) from T0 to T2 and decreased by 19% (p = 0.041) from T2 to T3 in patients, who experienced an episode during follow-up. CSF-8-oxoGuo did not show a statistically significant change in HC during the one-year follow-up. CSF and urine-8-oxoGuo levels correlated moderately. In conclusion, CSF oxidative stress marker of RNA damage 8-oxoGuo showed both state and trait dependence in BD and stability in HC. Central RNA damage may be a potential biomarker for BD.

scholarly journals Beneficial Effects of Glucagon-Like Peptide-1 (GLP-1) in Diabetes-Induced Retinal Abnormalities: Involvement of Oxidative Stress

Antioxidants ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 846
Author(s):  
Hugo Ramos ◽  
Patricia Bogdanov ◽  
Joel Sampedro ◽  
Jordi Huerta ◽  
Rafael Simó ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dna Repair ◽  
Glutathione Peroxidase ◽  
Antioxidant Defense ◽  
Topical Administration ◽  
Antioxidant Defense System ◽  
Eye Drops ◽  
Rna Damage ◽  
Glucagon Like Peptide ◽  
Glucagon Like Peptide 1

Background: Hyperglycemia-induced oxidative stress plays a key role in diabetic complications, including diabetic retinopathy. The main goal of this study was to assess whether the topical administration (eye drops) of glucagon-like peptide-1 (GLP-1) has any effect on oxidative stress in the retina. Methods: db/db mice were treated with eye drops of GLP-1 or vehicle for three weeks, with db/+ mice being used as control. Studies included the assessment by western blot of the antioxidant defense markers CuZnSOD, MnSOD, glutathione peroxidase and reductase; immunofluorescence measurements of DNA/RNA damage, nitro tyrosine and Ki67 and Babam2 proteins. Results: GLP-1 eye drops protected from oxidative stress by increasing the protein levels of glutathione reductase, glutathione peroxidase and CuZnSOD and MnSOD in diabetic retinas. This was associated with a significant reduction of DNA/RNA damage and the activation of proteins involved in DNA repair in the retina (Babam2) and Ki67 (a biomarker of cell proliferation). Conclusions: GLP-1 modulates the antioxidant defense system in the diabetic retina and has a neuroprotective action favoring DNA repair and neuron cells proliferation.

RNA damage and surveillance under oxidative stress

IUBMB Life ◽  
2006 ◽  
Vol 58 (10) ◽  
pp. 581-588 ◽  
Author(s):  
Zhongwei Li ◽  
Jinhua Wu ◽  
Christopher DeLeo
Keyword(s):  
Oxidative Stress ◽  
Rna Damage

scholarly journals Nutrient intake and influence on markers of oxidative stress in zoo managed male snow leopards (Uncia uncia)

2021 ◽  
Author(s):  
C J Iske ◽  
J R Herrick ◽  
C L Morris
Keyword(s):  
Oxidative Stress ◽  
Nutrient Intake ◽  
Sodium Intake ◽  
Reactive Species ◽  
Protein Carbonyls ◽  
Nutrient Concentrations ◽  
Rna Damage ◽  
Frequent Feeding ◽  
Snow Leopards ◽  
Gpx Activity

Abstract Oxidative stress (OS) results from overproduction of reactive species. Nutrient intake can contribute positively or negatively to OS and lack of established nutrient requirements for most exotic species managed in zoos exacerbates possibilities for nutrient imbalances that potentially could lead to reactive species production. The objective of this study was to evaluate the influence of nutrient intake and nutritional husbandry on markers of OS in male snow leopards (n = 14) maintained in U.S. facilities (n = 12). Diet samples and husbandry information were obtained and snow leopards were immobilized once for collection of blood. Samples were analyzed for chemical composition (diet and blood), antioxidant capacity (blood), and markers of OS (blood). Correlations between weekly nutrient intakes and markers of OS were analyzed by linear regression. Analyzed markers of OS included antioxidant enzymes (superoxide dismutase (SOD) and glutathione peroxidase (GPx)) and ferric reducing antioxidant potential (FRAP) that are protective against OS, and protein carbonyls (PC), thiobarbituric acid reactive substances (TBARS), and DNA/RNA damage that are indicative of oxidative damage. Weekly copper intake (10.1 – 80.2 mg) was negatively correlated with DNA/RNA damage (R 2 = 0.44; P = 0.01). Weekly sodium intake (4.4 – 12.7 g) was positively correlated with GPx activity (R 2 = 0.43; P = 0.04). More frequent feeding of whole prey (0.3 – 3 times/wk) was correlated with increased blood SOD activity (R 2 = 0.55; P < 0.01). In conclusion, greater dietary copper intake and more frequent feeding of whole prey may reduce OS in snow leopards. Dietary sodium intake and relationship with GPx activity should be further evaluated to determine benefit or detriment. No cause and effect can be inferred from our results, but our data suggest altering dietary form and nutrient concentrations may influence OS in snow leopards.

Lycopene alleviates sulfamethoxazole-induced hepatotoxicity in grass carp (Ctenopharyngodon idellus) via suppression of oxidative stress, inflammation and apoptosis

2020 ◽  
Vol 11 (10) ◽  
pp. 8547-8559
Author(s):  
Hongjing Zhao ◽  
Yu Wang ◽  
Mengyao Mu ◽  
Menghao Guo ◽  
Hongxian Yu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Secondary Metabolites ◽  
Human Health ◽  
Grass Carp ◽  
Aquatic Environment ◽  
Ctenopharyngodon Idellus

Antibiotics are used worldwide to treat diseases in humans and other animals; most of them and their secondary metabolites are discharged into the aquatic environment, posing a serious threat to human health.

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