Interactive effects of elevated temperature and CO2 on nitrate, urea, and dissolved inorganic carbon uptake by a coastal California, USA, microbial community

2017 ◽  
Vol 577 ◽  
pp. 49-65 ◽  
Author(s):  
JL Spackeen ◽  
RE Sipler ◽  
K Xu ◽  
AO Tatters ◽  
NG Walworth ◽  
...  
Keyword(s):  
Elevated Temperature ◽  
Microbial Community ◽  
Inorganic Carbon ◽  
Dissolved Inorganic Carbon ◽  
Interactive Effects ◽  
Carbon Uptake ◽  
Inorganic Carbon Uptake

Physiological responses to temperature and ocean acidification in tropical fleshy macroalgae with varying affinities for inorganic carbon

2020 ◽  
Author(s):  
Maureen Ho ◽  
James McBroom ◽  
Ellie Bergstrom ◽  
Guillermo Diaz-Pulido
Keyword(s):  
Ocean Acidification ◽  
Inorganic Carbon ◽  
Environmental Changes ◽  
Dissolved Inorganic Carbon ◽  
Physiological Responses ◽  
Marine Macroalgae ◽  
Interactive Effects ◽  
Carbon Uptake ◽  
Metabolic Rates ◽  
Δ13c Values

Abstract Marine macroalgae have variable carbon-uptake strategies that complicate predicting responses to environmental changes. In seawater, dissolved inorganic carbon availability can affect the underlying physiological mechanisms influencing carbon uptake. We tested the interactive effects of ocean acidification (OA) and warming on two HCO3−-users (Lobophora sp. and Amansia rhodantha), a predominately CO2-user (Avrainvillea nigricans), and a sole CO2-user (Plocamium hamatum) in the Great Barrier Reef, Australia. We examined metabolic rates, growth, and carbon isotope values (δ13C) in algae at 26, 28, or 30°C under ambient or elevated pCO2 (∼1000 µatm). Under OA, δ13C values for the HCO3−-users decreased, indicating less reliance on HCO3−, while δ13C values for CO2-users were unaffected. Both HCO3−-users decreased in growth across temperatures under ambient pCO2, but this negative effect was alleviated by OA at 30°C. A. nigricans lost biomass across all treatments and P. hamatum was most sensitive, with reduced survival in all physiological responses. Metabolic rates varied greatly to interacting temperature and OA and indicated a decoupling between the relationship of photosynthesis and growth. Furthermore, our findings suggest HCO3−-users are more responsive to future CO2 changes, and highlight examining carbon physiology to infer potential responses to interacting environmental stressors.

scholarly journals Proteomic and Mutant Analysis of the CO2 Concentrating Mechanism of Hydrothermal Vent Chemolithoautotroph Thiomicrospira crunogena

2017 ◽  
Vol 199 (7) ◽  
Author(s):  
Mary Mangiapia ◽  
Terry-René W. Brown ◽  
Dale Chaput ◽  
Edward Haller ◽  
Tara L. Harmer ◽  
...  
Keyword(s):  
Deep Sea ◽  
Inorganic Carbon ◽  
Dissolved Inorganic Carbon ◽  
Carbon Uptake ◽  
Primary Input ◽  
Content Type ◽  
Transporter Family ◽  
Thiomicrospira Crunogena ◽  
Inorganic Carbon Uptake ◽  
Autotrophic Microorganisms

ABSTRACT Many autotrophic microorganisms are likely to adapt to scarcity in dissolved inorganic carbon (DIC; CO2 + HCO3 − + CO3 2−) with CO2 concentrating mechanisms (CCM) that actively transport DIC across the cell membrane to facilitate carbon fixation. Surprisingly, DIC transport has been well studied among cyanobacteria and microalgae only. The deep-sea vent gammaproteobacterial chemolithoautotroph Thiomicrospira crunogena has a low-DIC inducible CCM, though the mechanism for uptake is unclear, as homologs to cyanobacterial transporters are absent. To identify the components of this CCM, proteomes of T. crunogena cultivated under low- and high-DIC conditions were compared. Fourteen proteins, including those comprising carboxysomes, were at least 4-fold more abundant under low-DIC conditions. One of these proteins was encoded by Tcr_0854; strains carrying mutated copies of this gene, as well as the adjacent Tcr_0853, required elevated DIC for growth. Strains carrying mutated copies of Tcr_0853 and Tcr_0854 overexpressed carboxysomes and had diminished ability to accumulate intracellular DIC. Based on reverse transcription (RT)-PCR, Tcr_0853 and Tcr_0854 were cotranscribed and upregulated under low-DIC conditions. The Tcr_0853-encoded protein was predicted to have 13 transmembrane helices. Given the mutant phenotypes described above, Tcr_0853 and Tcr_0854 may encode a two-subunit DIC transporter that belongs to a previously undescribed transporter family, though it is widespread among autotrophs from multiple phyla. IMPORTANCE DIC uptake and fixation by autotrophs are the primary input of inorganic carbon into the biosphere. The mechanism for dissolved inorganic carbon uptake has been characterized only for cyanobacteria despite the importance of DIC uptake by autotrophic microorganisms from many phyla among the Bacteria and Archaea. In this work, proteins necessary for dissolved inorganic carbon utilization in the deep-sea vent chemolithoautotroph T. crunogena were identified, and two of these may be able to form a novel transporter. Homologs of these proteins are present in 14 phyla in Bacteria and also in one phylum of Archaea, the Euryarchaeota. Many organisms carrying these homologs are autotrophs, suggesting a role in facilitating dissolved inorganic carbon uptake and fixation well beyond the genus Thiomicrospira.

Dissolved inorganic carbon uptake by Rhodomonas sp. and Isochrysis aff. galbana determined by a potentiometric technique

2005 ◽  
Vol 33 (2) ◽  
pp. 83-95 ◽  
Author(s):  
Tania K. Camiro-Vargas ◽  
J. Martín Hernández-Ayón ◽  
Enrique Valenzuela-Espinoza ◽  
Francisco Delgadillo-Hinojosa ◽  
Ramón Cajal-Medrano
Keyword(s):  
Inorganic Carbon ◽  
Dissolved Inorganic Carbon ◽  
Carbon Uptake ◽  
Inorganic Carbon Uptake

DISSOLVED INORGANIC CARBON UPTAKE IN TWO SUBMERGED MACROPHYTES FROM TAIHU LAKE, CHINA

2007 ◽  
Vol 31 (3) ◽  
pp. 490-496 ◽  
Author(s):  
XIAO Yue-E ◽  
◽  
CHEN Kai-Ning ◽  
DAI Xin-Bin ◽  
XU Xiao-Ming
Keyword(s):  
Inorganic Carbon ◽  
Taihu Lake ◽  
Dissolved Inorganic Carbon ◽  
Submerged Macrophytes ◽  
Carbon Uptake ◽  
Inorganic Carbon Uptake

scholarly journals Inorganic carbon uptake in a freshwater diatom, Asterionella formosa (Bacillariophyceae): from ecology to genomics

Phycologia ◽  
2021 ◽  
pp. 1-12
Author(s):  
Stephen C. Maberly ◽  
Brigitte Gontero ◽  
Carine Puppo ◽  
Adrien Villain ◽  
Ilenia Severi ◽  
...  
Keyword(s):  
Inorganic Carbon ◽  
Carbon Uptake ◽  
Asterionella Formosa ◽  
Inorganic Carbon Uptake ◽  
Freshwater Diatom
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