Particle selection and regulation of particle uptake by the slipper limpet Crepipatella fecunda

2013 ◽  
Vol 474 ◽  
pp. 167-177 ◽  
Author(s):  
OR Chaparro ◽  
JA Montory ◽  
SV Pereda ◽  
RJ Thompson ◽  
G Rivera ◽  
...  
Keyword(s):  
Particle Selection ◽  
Particle Uptake ◽  
Slipper Limpet

scholarly journals Screening for Effects of Inhaled Nanoparticles in Cell Culture Models for Prolonged Exposure

Nanomaterials ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 606
Author(s):  
Claudia Meindl ◽  
Kristin Öhlinger ◽  
Verena Zrim ◽  
Thomas Steinkogler ◽  
Eleonore Fröhlich
Keyword(s):  
Prolonged Exposure ◽  
A549 Cells ◽  
Cytokine Release ◽  
Transient Effects ◽  
Particle Uptake ◽  
Particle Accumulation ◽  
Low Concentrations ◽  
Culture Models ◽  
20 Nm ◽  
Inhaled Nanoparticles

Respiratory exposure of humans to environmental and therapeutic nanoparticles repeatedly occurs at relatively low concentrations. To identify adverse effects of particle accumulation under realistic conditions, monocultures of Calu-3 and A549 cells and co-cultures of A549 and THP-1 macrophages in the air–liquid interphase culture were exposed repeatedly to 2 µg/cm2 20 nm and 200 nm polystyrene particles with different functionalization. Particle accumulation, transepithelial electrical resistance, dextran (3–70 kDa) uptake and proinflammatory cytokine secretion were determined over 28 days. Calu-3 cells showed constant particle uptake without any change in barrier function and cytokine release. A549 cells preferentially ingested amino- and not-functionalized particles combined with decreased endocytosis. Cytokine release was transiently increased upon exposure to all particles. Carboxyl-functionalized demonstrated higher uptake and higher cytokine release than the other particles in the A549/THP-1 co-cultures. The evaluated respiratory cells and co-cultures ingested different amounts and types of particles and caused small (partly transient) effects. The data suggest that the healthy cells can adapt to low doses of non-cytotoxic particles.

Quantification of Particle Uptake by Conjunctiva-Associated Lymphoid Tissue (CALT) in Chickens

1991 ◽  
Vol 35 (1) ◽  
pp. 174 ◽  
Author(s):  
Andrew S. Fix ◽  
Lawrence H. Arp
Keyword(s):  
Lymphoid Tissue ◽  
Particle Uptake

Nanoparticles as carriers for oral peptide absorption: Studies on particle uptake and fate

1995 ◽  
Vol 36 (1-2) ◽  
pp. 39-46 ◽  
Author(s):  
Alexander T. Florence ◽  
Anya M. Hillery ◽  
Nasir Hussain ◽  
Praful U. Jani
Keyword(s):  
Peptide Absorption ◽  
Particle Uptake ◽  
Absorption Studies

Microfluidic optical sorting: particle selection in an optical lattice

2004 ◽  
Author(s):  
Michael P. MacDonald ◽  
Steven Neale ◽  
Lynn Paterson ◽  
Andrew Riches ◽  
Gabriel C. Spalding ◽  
...  
Keyword(s):  
Optical Lattice ◽  
Particle Selection ◽  
Optical Sorting

scholarly journals Initial events during phagocytosis by macrophages viewed from outside and inside the cell: membrane-particle interactions and clathrin.

1982 ◽  
Vol 94 (3) ◽  
pp. 613-623 ◽  
Author(s):  
J Aggeler ◽  
Z Werb
Keyword(s):  
Electron Microscopy ◽  
Plasma Membrane ◽  
Plasma Membranes ◽  
Cell Attachment ◽  
High Resolution Electron Microscopy ◽  
Peritoneal Macrophages ◽  
Coated Pits ◽  
Thin Sections ◽  
Particle Uptake ◽  
Freeze Dried

The initial events during phagocytosis of latex beads by mouse peritoneal macrophages were visualized by high-resolution electron microscopy of platinum replicas of freeze-dried cells and by conventional thin-section electron microscopy of macrophages postfixed with 1% tannic acid. On the external surface of phagocytosing macrophages, all stages of particle uptake were seen, from early attachment to complete engulfment. Wherever the plasma membrane approached the bead surface, there was a 20-nm-wide gap bridged by narrow strands of material 12.4 nm in diameter. These strands were also seen in thin sections and in replicas of critical-point-dried and freeze-fractured macrophages. When cells were broken open and the plasma membrane was viewed from the inside, many nascent phagosomes had relatively smooth cytoplasmic surfaces with few associated cytoskeletal filaments. However, up to one-half of the phagosomes that were still close to the cell surface after a short phagocytic pulse (2-5 min) had large flat or spherical areas of clathrin basketwork on their membranes, and both smooth and clathrin-coated vesicles were seen fusing with or budding off from them. Clathrin-coated pits and vesicles were also abundant elsewhere on the plasma membranes of phagocytosing and control macrophages, but large flat clathrin patches similar to those on nascent phagosomes were observed only on the attached basal plasma membrane surfaces. These resulted suggest that phagocytosis shares features not only with cell attachment and spreading but also with receptor-mediated pinocytosis.

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