scholarly journals Glyceraldehyde-3-phosphate dehydrogenase of Edwardsiella tarda has protective antigenicity against Vibrio anguillarum in Japanese flounder

2007 ◽  
Vol 75 ◽  
pp. 217-220 ◽  
Author(s):  
Y Liu ◽  
SI Oshima ◽  
K Kawai
Keyword(s):  
Vibrio Anguillarum ◽  
Japanese Flounder ◽  
Edwardsiella Tarda

scholarly journals Sensitive and Rapid Detection of Edwardsiellosis in Fish by a Loop-Mediated Isothermal Amplification Method

2004 ◽  
Vol 70 (1) ◽  
pp. 621-624 ◽  
Author(s):  
Ram Savan ◽  
Arisa Igarashi ◽  
Satoru Matsuoka ◽  
Masahiro Sakai
Keyword(s):  
Rapid Detection ◽  
Japanese Flounder ◽  
Isothermal Amplification ◽  
Edwardsiella Tarda ◽  
Fish Disease ◽  
Fish Pathogen ◽  
Loop Mediated Isothermal Amplification ◽  
Diagnostic Protocol ◽  
Amplification Method ◽  
Hemolysin Gene

ABSTRACT Here we report a rapid and sensitive method (using loop-mediated isothermal amplification [LAMP]) for the diagnosis of edwardsiellosis, a fish disease caused by Edwardsiella tarda, in Japanese flounder. A set of four primers was designed, and conditions for the detection were optimized for the detection of E. tarda in 45 min at 65°C. No amplification of the target hemolysin gene was detected in other related bacteria. When the LAMP primers were used, detection of edwardsiellosis in infected Japanese flounder kidney, and spleen and seawater cultures was possible. We have developed a rapid and sensitive diagnostic protocol for edwardsiellosis detection in fish. This is the first report of the application of LAMP for the diagnosis of a fish pathogen.

scholarly journals Antagonistic activity of terrestrial Streptomyces sp. VITNK9 against Gram negative bacterial pathogens affecting the fish and shellfish in aquaculture

2018 ◽  
Vol 53 (2) ◽  
pp. 171 ◽  
Author(s):  
Mohammed Ishaque Nabila ◽  
Krishnan Kannabiran
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Vibrio Harveyi ◽  
Bacterial Pathogens ◽  
Vibrio Anguillarum ◽  
Antagonistic Activity ◽  
Edwardsiella Tarda ◽  
Streptomyces Sp ◽  
Aeromonas Caviae ◽  
Fish And Shellfish

A total of 72 morphologically different actinomycetes isolates were isolated from samples collected at different regions of Vellore, Tamil Nadu, India and screened for its antibacterial activity against fish and shellfish pathogens. All actinomycetes isolates were screened for antibacterial activity by cross streak method against the selected fish and shellfish bacterial pathogens including Aeromonas caviae, Aeromonas hydroplila, Edwardsiella tarda, Vibrio anguillarum and Vibrio harveyi. Secondary screening of antagonistic isolates by well diffusion method leads to the identification of potential isolate. Culture conditions for the potential isolate were optimized for maximal growth and yield of the ethyl acetate (EA) crude extract. The potential isolate was characterized by molecular taxonomy and phylogeny and identified as Streptomyces species and named as Streptomyces sp. VITNK9. The 16S rDNA nucleotide sequence was searched through the GenBank database and showed 83% similarity to Streptomyces vinaceusdrappus. The EA extract prepared from Streptomyces sp. VITNK9 showed moderate antagonistic activity accessed by the formation of zone of growth inhibition against, Aeromonas caviae (15.33 mm), Aeromonas hydrophila (17.66 mm), Edwardsiella tarda (18.33 mm), Vibrio anguillarum (14.33 mm) and Vibrio harveyi (14.33 mm). The MIC value of EA extract was ranged between 0.03-0.125 mg mL-1. The GC-MS spectrum of the ethyl acetate extract revealed the presence of two major compounds, pyrrolo [1,2-A] pyrazine-1,4-Dione (56.67%) and Hexahydro-3-(2-Methylpropyl) (27.91%), respectively. The results of the study suggest that Streptomyces sp. VITNK9 is a potential source for antagonistic secondary metabolites against fish and shellfish bacterial pathogens.

scholarly journals A Serological Study on Edwardsiella tarda Strains Isolated from Diseased Japanese Flounder(Paralichthys olivaceus).

1994 ◽  
Vol 29 (4) ◽  
pp. 277 ◽  
Author(s):  
M. Mamnur Rashie ◽  
Takayuki Mekuchi ◽  
Toshihiro Nakai ◽  
Kiyokuni Muroga
Keyword(s):  
Paralichthys Olivaceus ◽  
Japanese Flounder ◽  
Edwardsiella Tarda ◽  
Serological Study
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