Diagnosis of lymphocystis disease in a novel host, the whitemouth croaker Micropogonias furnieri, associated with a putatively novel Lymphocystivirus species (LCDV-WC)

2020 ◽  
Vol 137 (3) ◽  
pp. 185-193 ◽  
Author(s):  
A Perretta ◽  
A Doszpoly ◽  
R Puentes ◽  
M Bessonart
Keyword(s):  
Lymphocystis Disease ◽  
Novel Host ◽  
Micropogonias Furnieri

scholarly journals Complete genome sequence and analysis of a novel lymphocystivirus detected in whitemouth croaker (Micropogonias furnieri): lymphocystis disease virus 4

2020 ◽  
Vol 165 (5) ◽  
pp. 1215-1218
Author(s):  
Andor Doszpoly ◽  
Győző L. Kaján ◽  
Rodrigo Puentes ◽  
Alejandro Perretta
Keyword(s):  
Disease Virus ◽  
Complete Genome ◽  
Virus Disease ◽  
Sequence Similarity ◽  
Amino Acid Sequence Similarity ◽  
Lymphocystis Disease Virus ◽  
Protein Coding ◽  
Lymphocystis Disease ◽  
Micropogonias Furnieri ◽  
Core Genes

Abstract A novel lymphocystivirus causing typical signs of lymphocystis virus disease in whitemouth croaker (Micropogonias furnieri) on the coast of Uruguay was detected and described recently. Based on genetic analysis of some partially sequenced core genes, the virus seemed to differ from previously described members of the genus Lymphocystivirus. In this study, using next-generation sequencing, the whole genome of this virus was sequenced and analysed. The complete genome was found to be 211,086 bp in size, containing 148 predicted protein-coding regions, including the 26 core genes that seem to have a homologue in every iridovirus genome sequenced to date. Considering the current species demarcation criteria for the family Iridoviridae (genome organization, G+C content, amino acid sequence similarity, and phylogenetic relatedness of the core genes), the establishment of a novel species (“Lymphocystis disease virus 4”) in the genus Lymphocystivirus is suggested.

scholarly journals Lymphocystic Disease in Wild Croaker (Micropogonias furnieri) and Flounder (Paralichthys orbignyanus)

2020 ◽  
pp. 29-36
Author(s):  
V. F. Pedrosa ◽  
M. C. Klosterhoff ◽  
A. F. F. De Medeiros ◽  
C. A. Paz-Villarraga ◽  
L. A. Romano
Keyword(s):  
Periodic Acid ◽  
Skin Lesions ◽  
Periodic Acid Schiff ◽  
Lymphocystis Disease ◽  
In The Wild ◽  
Macroscopic Findings ◽  
Hematoxylin And Eosin ◽  
Micropogonias Furnieri ◽  
The City ◽  
Paralichthys Orbignyanus

Lymphocystis disease has been reported worldwide in several species of freshwater fish and marine fish, naturally infected in the wild environment, or in intensive crops in farms. Nodular warty lesions of irregular surface were observed in the tegument and fins and mouth in a species of croaker (Micropogonias furnieri) caught in Cassino beach Rio Grande RS, Brazil and flounder (Paralichthys orbignyanus) caught in the city of San Clemente, Argentina. The skin lesions fragments were fixed in 20% buffered formalin, and the histological sections were stained with hematoxylin and eosin and Periodic Acid-Schiff (PAS), where microscopic alterations were visualized in the form of hyaline capsule with small basophilic structures in nodules and fibroblastic cells proliferation. The reported cases were based on the disease macroscopic findings characteristic of a lymphocystis disease, along with the histopathology, which confirmed the presence of the disease in the analyzed tissues.

The zoonotic potential of bat-borne coronaviruses

2020 ◽  
Vol 4 (4) ◽  
pp. 365-381
Author(s):  
Ny Anjara Fifi Ravelomanantsoa ◽  
Sarah Guth ◽  
Angelo Andrianiaina ◽  
Santino Andry ◽  
Anecia Gentles ◽  
...  
Keyword(s):  
Genetic Material ◽  
Field Research ◽  
Sympatric Species ◽  
Animal Origin ◽  
Mammalian Host ◽  
Past Century ◽  
The Past ◽  
Novel Host ◽  
Horseshoe Bat ◽  
Human Infections

Seven zoonoses — human infections of animal origin — have emerged from the Coronaviridae family in the past century, including three viruses responsible for significant human mortality (SARS-CoV, MERS-CoV, and SARS-CoV-2) in the past twenty years alone. These three viruses, in addition to two older CoV zoonoses (HCoV-229E and HCoV-NL63) are believed to be originally derived from wild bat reservoir species. We review the molecular biology of the bat-derived Alpha- and Betacoronavirus genera, highlighting features that contribute to their potential for cross-species emergence, including the use of well-conserved mammalian host cell machinery for cell entry and a unique capacity for adaptation to novel host environments after host switching. The adaptive capacity of coronaviruses largely results from their large genomes, which reduce the risk of deleterious mutational errors and facilitate range-expanding recombination events by offering heightened redundancy in essential genetic material. Large CoV genomes are made possible by the unique proofreading capacity encoded for their RNA-dependent polymerase. We find that bat-borne SARS-related coronaviruses in the subgenus Sarbecovirus, the source clade for SARS-CoV and SARS-CoV-2, present a particularly poignant pandemic threat, due to the extraordinary viral genetic diversity represented among several sympatric species of their horseshoe bat hosts. To date, Sarbecovirus surveillance has been almost entirely restricted to China. More vigorous field research efforts tracking the circulation of Sarbecoviruses specifically and Betacoronaviruses more generally is needed across a broader global range if we are to avoid future repeats of the COVID-19 pandemic.

Detection of lymphocystis disease virus (LCDV) in asymptomatic cultured gilt-head seabream (Sparus aurata, L.) using an immunoblot technique

2006 ◽  
Vol 113 (1-2) ◽  
pp. 137-141 ◽  
Author(s):  
I. Cano ◽  
M.C. Alonso ◽  
E. Garcia-Rosado ◽  
S. Rodriguez Saint-Jean ◽  
D. Castro ◽  
...  
Keyword(s):  
Disease Virus ◽  
Sparus Aurata ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease ◽  
Immunoblot Technique

Population structure and ontogenetic habitat use of Micropogonias furnieri in the Southwestern Atlantic Ocean inferred by otolith chemistry

2021 ◽  
Vol 240 ◽  
pp. 105953
Author(s):  
Esteban Avigliano ◽  
Nadia M. Alves ◽  
M. Rita Rico ◽  
Claudio O. Ruarte ◽  
Luciana D’Atri ◽  
...  
Keyword(s):  
Population Structure ◽  
Atlantic Ocean ◽  
Habitat Use ◽  
Otolith Chemistry ◽  
Southwestern Atlantic ◽  
Southwestern Atlantic Ocean ◽  
Micropogonias Furnieri

scholarly journals RNA m6A modification orchestrates a LINE-1–host interaction that facilitates retrotransposition and contributes to long gene vulnerability

Cell Research ◽  
2021 ◽  
Author(s):  
Feng Xiong ◽  
Ruoyu Wang ◽  
Joo-Hyung Lee ◽  
Shenglan Li ◽  
Shin-Fu Chen ◽  
...  
Keyword(s):  
Matrix Protein ◽  
Rna Binding ◽  
Rna Binding Proteins ◽  
Regulatory Elements ◽  
Host Interaction ◽  
Damage Repair ◽  
Novel Host ◽  
Cell Type Specific ◽  
Human Fetal Tissues ◽  
Long Genes

AbstractThe molecular basis underlying the interaction between retrotransposable elements (RTEs) and the human genome remains poorly understood. Here, we profiled N6-methyladenosine (m6A) deposition on nascent RNAs in human cells by developing a new method MINT-Seq, which revealed that many classes of RTE RNAs, particularly intronic LINE-1s (L1s), are strongly methylated. These m6A-marked intronic L1s (MILs) are evolutionarily young, sense-oriented to hosting genes, and are bound by a dozen RNA binding proteins (RBPs) that are putative novel readers of m6A-modified RNAs, including a nuclear matrix protein SAFB. Notably, m6A positively controls the expression of both autonomous L1s and co-transcribed L1 relics, promoting L1 retrotransposition. We showed that MILs preferentially reside in long genes with critical roles in DNA damage repair and sometimes in L1 suppression per se, where they act as transcriptional “roadblocks” to impede the hosting gene expression, revealing a novel host-weakening strategy by the L1s. In counteraction, the host uses the SAFB reader complex to bind m6A-L1s to reduce their levels, and to safeguard hosting gene transcription. Remarkably, our analysis identified thousands of MILs in multiple human fetal tissues, enlisting them as a novel category of cell-type-specific regulatory elements that often compromise transcription of long genes and confer their vulnerability in neurodevelopmental disorders. We propose that this m6A-orchestrated L1–host interaction plays widespread roles in gene regulation, genome integrity, human development and diseases.

scholarly journals Biologia reprodutiva de Micropogonias furnieri (Desmarest, 1823) (Teleostei, Sciaenidae): 2. Relação gonadossomática, comprimento e peso dos ovários como indicadores do período de desova

1987 ◽  
Vol 35 (2) ◽  
pp. 123-134 ◽  
Author(s):  
Victoria J Isaac-Nahum ◽  
Anna Emília A. de M Vazzoler
Keyword(s):  
Micropogonias Furnieri

Analisaram-se as variações da relaçao gonadossomática (RGS), do comprimento e do peso médios dos ovários por estádio de maturidade e durante um ano, procurando-se estabelecer indicadores quantitativos do grau de desenvolvimento gonadal e dos períodos de desova da população I de Micropogonias funieri. Constatou-se que, para fêmeas num mesmo estádio de maturidade, nao ocorre dependência entre valores individuais de RGS e peso total, sendo a RGS constante para cada estádio, o que permite sua utilização como indicador do grau de desenvolvimento gonadal. Ocorreram variações cíclicas paralelas nos valores dos três indicadores, cujos valores modais coincidem com os períodos de desova (outono, inverno e primavera-verão), o que nos permite utiliza-los como indicadores desses períodos.

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