High juvenile mortality in amphibians during overwintering related to fungal pathogen exposure

2018 ◽  
Vol 131 (1) ◽  
pp. 13-28 ◽  
Author(s):  
SL Rumschlag ◽  
MD Boone
Keyword(s):  
Fungal Pathogen ◽  
Juvenile Mortality ◽  
Pathogen Exposure

Studies on the Fungal Pathogen of Dutch Elm Disease

1981 ◽  
Vol 39 ◽  
pp. 400-401
Author(s):  
H.M. Mazzone ◽  
G. Wray ◽  
R. Zerillo
Keyword(s):  
Fungal Pathogen ◽  
Fungal Growth ◽  
Polymyxin B ◽  
The United States ◽  
Dutch Elm Disease ◽  
Effective Control ◽  
Sabouraud Agar ◽  
Total Inhibition ◽  
Zones Of Inhibition ◽  
Control Plate

The fungal pathogen of the Dutch elm disease (DED), Ceratocystis ulmi (Buisman) C. Moreau, has eluded effective control since its introduction in the United States more than sixty years ago. Our studies on DED include establishing biological control agents against C. ulmi. In this report we describe the inhibitory action of the antibiotic polymyxin B on the causal agent of DED.In screening a number of antibiotics against C. ulmi, we observed that filter paper discs containing 300 units (U) of polymyxin B (Difco Laboratories) per disc, produced zones of inhibition to the fungus grown on potato dextrose agar or Sabouraud agar plates (100mm x 15mm), Fig. 1a. Total inhibition of fungal growth on a plate occurred when agar overlays containing fungus and antibiotic (polymyxin B sulfate, ICN Pharmaceuticals, Inc.) were poured on the underlying agar growth medium. The agar overlays consisted of the following: 4.5 ml of 0.7% agar, 0.5 ml of fungus (control plate); 4.0 ml of 0.7% agar, 0.5 ml of fungus, 0.5 ml of polymyxin B sulfate (77,700 U). Fig. 1, b and c, compares a control plate and polymyxin plate after seven days.

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