Goldfish Carassius auratus susceptibility to viral hemorrhagic septicemia virus genotype IVb depends on exposure route

2015 ◽  
Vol 115 (1) ◽  
pp. 25-36 ◽  
Author(s):  
RG Getchell ◽  
T Erkinharju ◽  
AO Johnson ◽  
BW Davis ◽  
EE Hatch ◽  
...  
Keyword(s):  
Carassius Auratus ◽  
Viral Hemorrhagic Septicemia Virus ◽  
Virus Genotype ◽  
Exposure Route ◽  
Viral Hemorrhagic Septicemia ◽  
Goldfish Carassius Auratus ◽  
Genotype Ivb

scholarly journals Fin and gill biopsies are effective nonlethal samples for detection of Viral hemorrhagic septicemia virus genotype IVb

2013 ◽  
Vol 25 (2) ◽  
pp. 203-209 ◽  
Author(s):  
Emily R. Cornwell ◽  
Chelsea A. Bellmund ◽  
Geoffrey H. Groocock ◽  
Po Ting Wong ◽  
Katherine L. Hambury ◽  
...  
Keyword(s):  
Viral Hemorrhagic Septicemia Virus ◽  
Virus Genotype ◽  
Viral Hemorrhagic Septicemia ◽  
Genotype Ivb

scholarly journals Typing of viral hemorrhagic septicemia virus by monoclonal antibodies

2012 ◽  
Vol 93 (12) ◽  
pp. 2546-2557 ◽  
Author(s):  
Takafumi Ito ◽  
Jun Kurita ◽  
Motohiko Sano ◽  
Helle Frank Skall ◽  
Niels Lorenzen ◽  
...  
Keyword(s):  
Antigenic Determinants ◽  
Viral Hemorrhagic Septicemia Virus ◽  
N Protein ◽  
Viral Hemorrhagic Septicemia ◽  
Genotype Iii ◽  
P Protein ◽  
The Usa ◽  
Nucleoprotein N ◽  
Genotype Ivb ◽  
Reaction Patterns

Seven mAbs with specific reaction patterns against each of the four genotypes and eight subtypes of viral hemorrhagic septicemia virus (VHSV) were produced, aiming to establish an immunoassay for typing VHSV isolates according to their genotype. Among the mAbs, VHS-1.24 reacted with all genotypes except genotype Ie, whilst mAb VHS-9.23 reacted with all genotypes except genotype III. mAb VHS-3.80 reacted with genotypes Ib, Ic, Id and II. mAb VHS-7.57 reacted with genotypes II and IVa, and mAb VHS-5.18 with genotype Ib only. Interestingly, mAb VHS-3.75 reacted with all of the genotype III isolates except a rainbow trout-pathogenic isolate from the west coast of Norway, and reacted in addition with the IVb isolate, CA-NB00-01, from the east coast of the USA. Finally, mAb VHS-1.88 reacted with all genotype IVb isolates from the Great Lakes, but not with CA-NB00-01. In conclusion, we can distinguish between all four genotypes and between five of eight subtypes of VHSV by testing isolates in immunoassay using a panel of nine mAbs. By Western blotting and transfection of cell cultures, it was shown that mAb VHS-1.24 recognized an epitope on the viral phosphoprotein (P), whilst all others recognized antigenic determinants on the nucleoprotein (N). From amino acid alignments of the various genotypes and subtypes of VHSV isolates, it was possible to determine the epitope specificity of mAb VHS-1.24 to be aa 32–34 in the P-protein; the specificities of mAbs VHS-3.80, VHS-7.57 and VHS-3.75 were found to be aa 43 and 45–48, aa 117 and 121, and aa 103, 118 and 121 of the N-protein, respectively.

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