Species-specific oligonucleotide probe for detection of Bonamia exitiosa (Haplosporidia) using in situ hybridisation assay

2014 ◽  
Vol 110 (1) ◽  
pp. 81-91 ◽  
Author(s):  
A Ramilo ◽  
A Villalba ◽  
E Abollo
Keyword(s):  
Oligonucleotide Probe ◽  
In Situ Hybridisation ◽  
Specific Oligonucleotide Probe ◽  
Species Specific

scholarly journals In situ hybridisation for the detection of Leishmania species in paraffin wax-embedded canine tissues using a digoxigenin-labelled oligonucleotide probe

2011 ◽  
Vol 169 (20) ◽  
pp. 525-525 ◽  
Author(s):  
N. Dinhopl ◽  
M. M. Mostegl ◽  
B. Richter ◽  
N. Nedorost ◽  
A. Maderner ◽  
...  
Keyword(s):  
Oligonucleotide Probe ◽  
In Situ Hybridisation ◽  
Leishmania Species ◽  
Paraffin Wax

Application of oligonucleotide probes for the detection of Thiothrix spp. in activated sludge plants treating paper and board mill wastes

2002 ◽  
Vol 46 (1-2) ◽  
pp. 559-564 ◽  
Author(s):  
S.B. Kim ◽  
M. Goodfellow ◽  
J. Kelly ◽  
G.S. Saddler ◽  
A.C. Ward
Keyword(s):  
Activated Sludge ◽  
Bacterial Population ◽  
Oligonucleotide Probe ◽  
In Situ Hybridisation ◽  
Filamentous Bacteria ◽  
Phylogenetic Groups ◽  
Investigation Period ◽  
Mixed Populations ◽  
Conventional Microscopy

Filamentous bacteria belonging to the genus Thiothrix were detected in activated sludge samples using the fluorescent in situ hybridisation (FISH) technique. A 16S rRNA-targeted oligonucleotide probe was developed for the detection of members of the T. fructosivorans group, and the performance of probe TNI for the detection of Thiothrix nivea group was enhanced by using an unlabeled competitor. A set of 5 probes covering all phylogenetic groups of Thiothrix were used to examine samples taken from selected activated sludge plants treating paper and board mill wastes. Members of the T. eikelboomii group formed the predominant filamentous bacterial population in plants experiencing poor sludge settleability, whereas members of the T. nivea group were commonly found but not dominantly in the remaining plants. Members of the T. fructosivorans group were not detected at any significant level in any of the samples. The distribution of the main Thiothrix types remained unchanged throughout the investigation period. It was evident that mixed populations of Thiothrix spp. were present in all activated sludge samples investigated, the observed differences were in the relative abundance of the various groups. These findings were supported by the results obtained using conventional microscopy.

Quantitative fluorescent in-situ hybridization: a hypothesized competition mode between two dominant bacteria groups in hydrogen-producing anaerobic sludge processes

2009 ◽  
Vol 59 (10) ◽  
pp. 1901-1909 ◽  
Author(s):  
C.-L. Huang ◽  
C.-C. Chen ◽  
C.-Y. Lin ◽  
W.-T. Liu
Keyword(s):  
In Situ Hybridization ◽  
Microbial Communities ◽  
Fluorescent In Situ Hybridization ◽  
Oligonucleotide Probe ◽  
Municipal Sewage Sludge ◽  
Anaerobic Sludge ◽  
Competition Mode ◽  
Specific Oligonucleotide Probe ◽  
Dominant Bacteria

Two hydrogen-producing continuous flow stirred tank reactors (CSTRs) fed respectively with glucose and sucrose were investigated by polymerase chain reaction-denatured gradient gel electrophoresis (PCR-DGGE) and fluorescent in-situ hybridization (FISH). The substrate was fed in a continuous mode decreased from hydraulic retention time (HRT) 10 hours to 6, 5, 4, 3, and 2 hours. Quantitative fluorescent in-situ hybridization (FISH) observations further demonstrated that two morphotypes of bacteria dominated both microbial communities. One was long rod bacteria which can be targeted either by Chis150 probe designed to hybridize the gram positive low G + C bacteria or the specific oligonucleotide probe Lg10-6. The probe Lg10-6, affiliated with Clostridium pasteurianum, was designed and then checked with other reference organisms. The other type, unknown group, which cannot be detected by Chis150 was curved rod bacteria. Notably, the population ratios of the two predominant groups reflected the different operational performance of the two reactors, such as hydrogen producing rates, substrate turnover rates and metabolites compositions. Therefore, a competition mode of the two dominant bacteria groups was hypothesized. In the study, 16S rRNA-based gene library of hydrogen-producing microbial communities was established. The efficiency of hydrogen yields was correlated with substrates (glucose or sucrose), HRT, metabolites compositions (acetate, propionate, butyrate and ethanol), thermal pre-treatment (seed biomass was heated at 100°C for 45 minutes), and microbial communities in the bioreactor, not sludge sources (municipal sewage sludge, alcohol-processing sludge, or bean-processing sludge). The designed specific oligonucleotide probe Lg10-6 also provides us a useful and fast molecular tool to screen hydrogen-producing microbial communities in the future research.

scholarly journals Preliminary study using species-specific oligonucleotide probe for rRNA of Bilophila wadsworthia.

1994 ◽  
Vol 32 (10) ◽  
pp. 2510-2513 ◽  
Author(s):  
F L Sapico ◽  
D Reeves ◽  
H M Wexler ◽  
J Duncan ◽  
K H Wilson ◽  
...  
Keyword(s):  
Oligonucleotide Probe ◽  
Specific Oligonucleotide Probe ◽  
Preliminary Study ◽  
Species Specific

Revival of the Species Lactobacillus lindneri and the Design of a Species Specific Oligonucleotide Probe

1996 ◽  
Vol 19 (3) ◽  
pp. 322-325 ◽  
Author(s):  
Werner Back ◽  
Ingrid Bohak ◽  
Matthias Ehrmann ◽  
Wolfgang Ludwig ◽  
Karl Heinz Schleifer
Keyword(s):  
Oligonucleotide Probe ◽  
Specific Oligonucleotide Probe ◽  
Species Specific

scholarly journals Chromosome Mapping of 5S Ribosomal Genes in Indo-Pacific and Atlantic Muraenidae: Comparative Analysis by Dual Colour Fluorescence In Situ Hybridisation

Genes ◽  
2020 ◽  
Vol 11 (11) ◽  
pp. 1319
Author(s):  
Elisabetta Coluccia ◽  
Federica Deidda ◽  
Cinzia Lobina ◽  
Riccardo Melis ◽  
Cristina Porcu ◽  
...  
Keyword(s):  
Gene Family ◽  
In Situ Hybridisation ◽  
5S Rdna ◽  
Chromosome Mapping ◽  
Ribosomal Gene ◽  
Ribosomal Genes ◽  
Chromosomal Mapping ◽  
Fluorescence In Situ Hybridisation ◽  
Species Specific

The Muraenidae is one of the largest and most complex anguilliform families. Despite their abundance and important ecological roles, morays are little studied, especially cytogenetically, and both their phylogenetic relationships and the taxonomy of their genera are controversial. With the aim of extending the karyology of this fish group, the chromosomal mapping of the 5S ribosomal gene family was performed on seven species belonging to the genera Muraena and Gymnothorax from both the Atlantic and Pacific oceans. Fluorescence in situ hybridisation (FISH) experiments were realized using species-specific 5S rDNA probes; in addition, two-colour FISH was performed to investigate the possible association with the 45S ribosomal gene family. Multiple 5S rDNA clusters, located either in species-specific or in possibly homoeologous chromosomes, were found. Either a syntenic or different chromosomal location of the two ribosomal genes was detected. Our results revealed variability in the number and location of 5S rDNA clusters and confirmed a substantial conservation of the number and location of the 45S rDNA.

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