Expression analysis and identification of antimicrobial peptide transcripts from six North American frog species

2013 ◽  
Vol 104 (3) ◽  
pp. 225-236 ◽  
Author(s):  
LS Robertson ◽  
GM Fellers ◽  
JM Marranca ◽  
PM Kleeman
Keyword(s):  
Antimicrobial Peptide ◽  
Expression Analysis ◽  
North American ◽  
Frog Species ◽  
American Frog

TOXICITY OF GLYPHOSATE-BASED PESTICIDES TO FOUR NORTH AMERICAN FROG SPECIES

10.1897/03-71 ◽  
2004 ◽  
Vol 23 (8) ◽  
pp. 1928 ◽  
Author(s):  
Christina M. Howe ◽  
Michael Berrill ◽  
Bruce D. Pauli ◽  
Caren C. Helbing ◽  
Kate Werry ◽  
...  
Keyword(s):  
North American ◽  
Frog Species ◽  
American Frog

Localization of the nucleolar organizer and heterochromatin evolution in three European brown frogs: Rana dalmatina, Rana graeca, Rana iberica

1989 ◽  
Vol 10 (4) ◽  
pp. 387-396 ◽  
Author(s):  
Gaetano Odierna
Keyword(s):  
Genome Size ◽  
North American ◽  
Constitutive Heterochromatin ◽  
Nucleolar Organizer ◽  
Frog Species ◽  
Brown Frog ◽  
Rana Dalmatina

AbstractThis study investigates the karyotypes, genome sizc, constitutive heterochromatin, and NOR localization in three European brown frog species: Rana iberica, R. dalmatina and R. graeca. The three species all possess a karyotype of 26 chromosomes. The genome size is respectively 6.9, 9.7 and 11.3 pg/N in R. iberica, R. dalmatina and R. graeca. Constitutive heterochromatin is 1.7% in R. iberica, 3.1 % in R. dalmatina and 7.0% in R. graeca. The NOR is located on the long arm of the 10th chromosome in R. iberica and R. graeca, whereas it is pericentromerically localized on the short arm of the 3rd chromosome in R. dalmatina. A single NOR extrasite is found in R. graeca. Results suggest that European brown frogs have evolved independently from North American and Asian groups. Moreover, the variation observed in constitutive heterochromatin is informative of different amplification levels of satellite-like DNA. Data also suggest that in R. dalmatina NOR localization on the 3rd chromosome may be derivcd by amplification of a pre-existing NOR site.

THE USE OF THE MALE NORTH AMERICAN FROG (RANA PIPIENS) IN THE DIAGNOSIS OF PREGNANCY1

Endocrinology ◽  
1948 ◽  
Vol 42 (4) ◽  
pp. 237-243 ◽  
Author(s):  
STANLEY L. ROBBINS ◽  
FREDERIC PARKER
Keyword(s):  
North American ◽  
Rana Pipiens ◽  
American Frog

North American Frog Lung Flukes

1915 ◽  
Vol 34 (4) ◽  
pp. 203 ◽  
Author(s):  
William Walter Cort
Keyword(s):  
North American ◽  
American Frog

scholarly journals It’s okay to be green: Draft genome of the North American bullfrog (Rana [Lithobates] catesbeiana)

2017 ◽  
Author(s):  
S. Austin Hammond ◽  
René L. Warren ◽  
Benjamin P. Vandervalk ◽  
Erdi Kucuk ◽  
Hamza Khan ◽  
...  
Keyword(s):  
North American ◽  
Draft Genome ◽  
Food Sources ◽  
Model Organisms ◽  
Population Declines ◽  
American Bullfrog ◽  
The North ◽  
Long Reads ◽  
Frog Species ◽  
Lithobates Catesbeiana

AbstractFrogs play important ecological roles as sentinels, insect control and food sources. Several species are important model organisms for scientific research to study embryogenesis, development, immune function, and endocrine signaling. The globally-distributed Ranidae (true frogs) are the largest frog family, and have substantial evolutionary distance from the model laboratory Xenopus frog species. Consequently, the extensive Xenopus genomic resources are of limited utility for Ranids and related frog species. More widely applicable amphibian genomic data is urgently needed as more than two-thirds of known species are currently threatened or are undergoing population declines.Herein, we report on the first genome sequence of a Ranid species, an adult male North American bullfrog (Rana [Lithobates] catesbeiana). We assembled high-depth Illumina reads (66-fold coverage), into a 5.8 Gbp (NG50 = 57.7 kbp) draft genome using ABySS v1.9.0. The assembly was scaffolded with LINKS and RAILS using pseudo-long-reads from targeted denovo assembler Kollector and Illumina Synthetic Long-Reads, as well as reads from long fragment (MPET) libraries. We predicted over 22,000 protein-coding genes using the MAKER2 pipeline and identified the genomic loci of 6,227 candidate long noncoding RNAs (IncRNAs) from a composite reference bullfrog transcriptome. Mitochondrial sequence analysis supported Lithobates as a subgenus of Rana. RNA-Seq experiments identified ~6,000 thyroid hormone– responsive transcripts in the back skin of premetamorphic tadpoles; the majority of which regulate DNA/RNA processing. Moreover, 1/6th of differentially-expressed transcripts were putative lncRNAs. Our draft bullfrog genome will serve as a useful resource for the amphibian research community.

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