scholarly journals Stimulation of carp Cyprinus carpio lymphocytes in vitro by the blood fluke Sanguinicola inermis (Trematoda:Sanguinicolidae)

1996 ◽  
Vol 25 ◽  
pp. 87-93 ◽  
Author(s):  
DT Richards ◽  
D Hoole ◽  
JW Lewis ◽  
E Ewens ◽  
C Arme
Keyword(s):  
Cyprinus Carpio ◽  
Blood Fluke ◽  
Carp Cyprinus Carpio ◽  
Stimulation Of

Optimization and kinetics of in vitro stimulation of carp (Cyprinus carpio L.) leukocytes

1982 ◽  
Vol 3 (3) ◽  
pp. 325-343 ◽  
Author(s):  
E.W. Liewes ◽  
R.H. Van Dam ◽  
M.G. Vos-Maas ◽  
R. Bootsma
Keyword(s):  
Cyprinus Carpio ◽  
Carp Cyprinus Carpio ◽  
Kinetics Of ◽  
Stimulation Of

scholarly journals In vitro cultivation of Trypanoplasma borreli (Protozoa:Kinetoplastida), a parasite from the blood of common carp Cyprinus carpio

2000 ◽  
Vol 41 ◽  
pp. 195-201 ◽  
Author(s):  
D Steinhagen ◽  
W Hedderich ◽  
A Skouras ◽  
JP Scharsack ◽  
J Schuberth ◽  
...  
Keyword(s):  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Cultivation ◽  
Common Carp Cyprinus Carpio ◽  
Carp Cyprinus Carpio

scholarly journals The in vitro toxicity of atrazine on kinematics and DNA fragmentation in common carp (Cyprinus carpio) sperm cells

2019 ◽  
Vol 70 (3) ◽  
pp. 1639
Author(s):  
M.E. ÖZGÜR
Keyword(s):  
Dna Fragmentation ◽  
Common Carp ◽  
Cyprinus Carpio ◽  
In Vitro Toxicity ◽  
Computer Assisted ◽  
Sperm Cells ◽  
Common Carp Cyprinus Carpio ◽  
Significant Difference ◽  
Carp Cyprinus Carpio

This study investigated the in vitro effects of different concentrations of Atrazine (0.001, 0.01, 0.1, 0.5 mg/L) added to motile and immotile solutions on kinematics quality of sperm cells of common carp, Cyprinus carpio, which is a fish of economic significance. The kinematics of the sperm cells was analyzed by a computer-assisted sperm analysis system (CASA). As a result of the study, while there was a significant difference (P < 0.05) between the groups in terms of the VSL (μm/s) and VCL (μm/s) values after the Atrazine-added immotile solution’s (IMS) and incubation for 3 hours, there was a significant difference (P < 0.05) in only the VSL values directly activated by the Atrazine-added motile solution (MS). DNA fragmentation was evident but not in higher numbers in the 0.1 mg/L atrazine group. Finally, it was determined the effective concentration (EC50) values of the VSL value of the motile and immotile solution as 0.34 mg/L and 0.03 mg/L, respectively.

Effects of Bothriocephalus acheilognathi on the polarization response of pronephric leucocytes of carp, Cyprinus carpio

2000 ◽  
Vol 74 (3) ◽  
pp. 253-257 ◽  
Author(s):  
P. Nie ◽  
D. Hoole
Keyword(s):  
Cyprinus Carpio ◽  
Infected Fish ◽  
Polarization Response ◽  
Leucocyte Migration ◽  
Significant Difference ◽  
Carp Cyprinus Carpio ◽  
Bothriocephalus Acheilognathi ◽  
Carp Serum

AbstractAn in vitro assay was used to examine the effect of Bothriocephalus acheilognathi Yamaguti, 1934 (Cestoda: Pseudophyllidea) on the polarization response of pronephric leucocytes of carp, Cyprinus carpio. Leucocytes, isolated from naive, naturally-infected fish and carp injected intraperitoneally with cestode extracts, were exposed to parasite extracts (protein concentrations 0–10.0 μg ml-1), for up to 24 h in the presence or absence of carp serum. In general, polarization responses of the pronephric leucocytes, primarily neutrophils and eosinophils, increased with incubation time although there was no significant difference in the response induced by the different protein concentrations. Differences in the polarization response were, however, observed in naive, naturally infected and injected fish and the cells responded differently in the presence and absence of carp serum. In the absence of carp serum the polarization response of pronephric leucocytes in vitro was significantly reduced with cells obtained from injected and naturally infected fish compared with those obtained from naive carp. This suppression of leucocyte migration was however reduced by the addition of carp serum to the in vitro system. The role of this interaction between the possible suppression of polarization induced by the parasite and stimulation by serum is discussed.

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