scholarly journals Monoclonal antibodies against 27.8 kDa protein receptor efficiently block lymphocystis disease virus infection in flounder Paralichthys olivaceus gill cells

2012 ◽  
Vol 100 (1) ◽  
pp. 19-27 ◽  
Author(s):  
XZ Sheng ◽  
M Wang ◽  
J Xing ◽  
WB Zhan
Keyword(s):  
Monoclonal Antibodies ◽  
Virus Infection ◽  
Disease Virus ◽  
Paralichthys Olivaceus ◽  
Lymphocystis Disease Virus ◽  
Protein Receptor ◽  
Gill Cells ◽  
Lymphocystis Disease

scholarly journals Development and Characterization of Monoclonal Antibodies to the 32 kDa Viral Attachment Protein of Lymphocystis Disease Virus and Their Neutralizing Ability in Vitro

2018 ◽  
Vol 19 (9) ◽  
pp. 2536 ◽  
Author(s):  
Ying Zhong ◽  
Xiaoqian Tang ◽  
Xiuzhen Sheng ◽  
Jing Xing ◽  
Wenbin Zhan
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Paralichthys Olivaceus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Dilution Method ◽  
Attachment Protein ◽  
Lymphocystis Disease Virus ◽  
Viral Attachment ◽  
Lymphocystis Disease

In previous research, a 32 kDa protein in lymphocystis disease virus (LCDV) was identified as viral attachment protein (VAP) that specifically interacted with the 27.8 kDa cellular receptor from flounder Paralichthys olivaceus gill (FG) cells, and the recombinant VAP (rVAP) was expressed in Escherichia coli strain BL21 (DE3). In this study, monoclonal antibodies (MAbs) against 32 kDa VAP are produced by immunization of BALB/c mice with the rVAP. Seven hybridoma secreting MAbs were screened by enzyme-linked immunosorbent assay, five of which designated as 1C6, 1C8, 3B5, 3D11 and 3H10 are cloned by the limiting dilution method, depending on the strongly positive results of ELISA. Western blotting analysis shows that the five MAbs can specifically react with the 32 kDa protein of LCDV and the purified 50 kDa rVAP, and the subtype of the MAbs is identified as IgG. Immunofluorescence results demonstrate that the specific fluorescence signals for LCDV appear in the cytoplasm of FG cells at 24 h post LCDV infection. Neutralization assay results indicate that pre-incubations of LCDV with the five MAbs can significantly decrease the LCDV copy numbers and delay the development of the cytopathic effect in FG cells, revealing that the five MAbs can neutralize the LCDV particles and block viral infection in vitro. The neutralizing MAbs against 32 kDa VAP would be useful for the study on the LCDV–host interaction and might be promising inhibitors of LCDV infection in fish.

Proteomic aspects of infection by lymphocystis disease virus in Japanese flounder (Paralichthys olivaceus)

2011 ◽  
Vol 29 (3) ◽  
pp. 603-608
Author(s):  
Mingqing Xing ◽  
Xiuqin Sun ◽  
Fengrong Zheng ◽  
Lingyun Qu ◽  
Xuguang Hong ◽  
...  
Keyword(s):  
Disease Virus ◽  
Paralichthys Olivaceus ◽  
Japanese Flounder ◽  
Lymphocystis Disease Virus ◽  
Lymphocystis Disease
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