scholarly journals Yellow-head virus of Penaeus monodon is an RNA virus

1995 ◽  
Vol 22 ◽  
pp. 45-50 ◽  
Author(s):  
C Wongteerasupaya ◽  
S Sriurairatana ◽  
JE Vickers ◽  
A Akrajamorn ◽  
V Boonsaeng ◽  
...  
Keyword(s):  
Rna Virus ◽  
Penaeus Monodon ◽  
Yellow Head Virus

scholarly journals Identification and Characterization of a Penaeus monodon Lymphoid Cell-Expressed Receptor for the Yellow Head Virus

2006 ◽  
Vol 80 (1) ◽  
pp. 262-269 ◽  
Author(s):  
Wanchai Assavalapsakul ◽  
Duncan R. Smith ◽  
Sakol Panyim
Keyword(s):  
Rna Virus ◽  
Virus Entry ◽  
Penaeus Monodon ◽  
Penaeid Shrimp ◽  
Lymphoid Organ ◽  
Receptor Protein ◽  
Northern Analysis ◽  
Virus Binding ◽  
Degenerate Primers ◽  
Yellow Head Virus

ABSTRACT The yellow head virus is a positive-sense, single-stranded RNA virus that causes significant mortality in farmed penaeid shrimp. This study sought to isolate and characterize the receptor protein used by the virus to gain entry into Penaeus monodon Oka (lymphoid) organ cells, a primary target of yellow head virus infections. Virus overlay protein binding assay on Oka organ membrane preparations identified a 65-kDa protein, and antibodies raised against this protein inhibited virus entry in primary Oka cell cultures by approximately 80%. N-terminal sequence analysis of the 65-kDa protein generated a 17-amino acid peptide fragment which was used to design degenerate primers that amplified a 1.5-kbp product from Oka organ total RNA, which was cloned and sequenced. Northern analysis and PCR were used to confirm a single RNA transcript that was expressed in most tissues. Subsequently, the mature cDNA was recloned and the expressed protein shown to cross-react with the antibody raised against the original virus binding band. Down regulation of the message through double-stranded RNA-mediated RNA interference silencing resulted in the complete inhibition of virus entry. While the identity of the clone remains unknown, it nevertheless represents the first invertebrate Nidovirus receptor isolated to date.

Down-regulation of defender against apoptotic death (DAD1) after yellow head virus (YHV) challenge in black tiger shrimp Penaeus monodon

2008 ◽  
Vol 24 (2) ◽  
pp. 173-179 ◽  
Author(s):  
Sudkhate Molthathong ◽  
Saengchan Senapin ◽  
Sirawut Klinbunga ◽  
Narongsak Puanglarp ◽  
Jiraporn Rojtinnakorn ◽  
...  
Keyword(s):  
Penaeus Monodon ◽  
Down Regulation ◽  
Yellow Head Virus ◽  
Black Tiger Shrimp ◽  
Apoptotic Death ◽  
Tiger Shrimp

scholarly journals A Novel RNA Virus, Macrobrachium rosenbergii Golda Virus (MrGV), Linked to Mass Mortalities of the Larval Giant Freshwater Prawn in Bangladesh

Viruses ◽  
2020 ◽  
Vol 12 (10) ◽  
pp. 1120
Author(s):  
Chantelle Hooper ◽  
Partho P. Debnath ◽  
Sukumar Biswas ◽  
Ronny van Aerle ◽  
Kelly S. Bateman ◽  
...  
Keyword(s):  
Virus Assembly ◽  
Rna Virus ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
The Novel ◽  
Protein Motifs ◽  
Giant Freshwater Prawn ◽  
Yellow Head Virus ◽  
Novel Virus ◽  
Mass Mortalities

Mass mortalities of the larval stage of the giant freshwater prawn, Macrobrachium rosenbergii, have been occurring in Bangladesh since 2011. Mortalities can reach 100% and have resulted in an 80% decline in the number of hatcheries actively producing M. rosenbergii. To investigate a causative agent for the mortalities, a disease challenge was carried out using infected material from a hatchery experiencing mortalities. Moribund larvae from the challenge were prepared for metatranscriptomic sequencing. De novo virus assembly revealed a 29 kb single-stranded positive-sense RNA virus with similarities in key protein motif sequences to yellow head virus (YHV), an RNA virus that causes mass mortalities in marine shrimp aquaculture, and other viruses in the Nidovirales order. Primers were designed against the novel virus and used to screen cDNA from larvae sampled from hatcheries in the South of Bangladesh from two consecutive years. Larvae from all hatcheries screened from both years were positive by PCR for the novel virus, including larvae from a hatchery that at the point of sampling appeared healthy, but later experienced mortalities. These screens suggest that the virus is widespread in M. rosenbergii hatchery culture in southern Bangladesh, and that early detection of the virus can be achieved by PCR. The hypothesised protein motifs of Macrobrachium rosenbergii golda virus (MrGV) suggest that it is likely to be a new species within the Nidovirales order. Biosecurity measures should be taken in order to mitigate global spread through the movement of post-larvae within and between countries, which has previously been linked to other virus outbreaks in crustacean aquaculture.

Activation of PmRelish from Penaeus monodon by yellow head virus

2015 ◽  
Vol 42 (2) ◽  
pp. 335-344 ◽  
Author(s):  
Suwattana Visetnan ◽  
Premruethai Supungul ◽  
Ikuo Hirono ◽  
Anchalee Tassanakajon ◽  
Vichien Rimphanitchayakit
Keyword(s):  
Penaeus Monodon ◽  
Yellow Head Virus

Rab5, an early endosomal protein required for yellow head virus infection of Penaeus monodon

Aquaculture ◽  
2016 ◽  
Vol 459 ◽  
pp. 43-53 ◽  
Author(s):  
Pratsaneeyaporn Posiri ◽  
Sakol Panyim ◽  
Chalermporn Ongvarrasopone
Keyword(s):  
Virus Infection ◽  
Penaeus Monodon ◽  
Yellow Head Virus

scholarly journals Evidence for apoptosis correlated with mortality in the giant black tiger shrimp Penaeus monodon infected with yellow head virus

2002 ◽  
Vol 48 ◽  
pp. 79-90 ◽  
Author(s):  
K Khanobdee ◽  
C Soowannayan ◽  
TW Flegel ◽  
S Ubol ◽  
B Withyachumnarnkul
Keyword(s):  
Penaeus Monodon ◽  
Yellow Head Virus ◽  
Black Tiger Shrimp ◽  
Tiger Shrimp

scholarly journals A novel RNA virus, Macrobrachium rosenbergii Golda virus (MrGV), linked to mass mortalities of the larval giant freshwater prawn in Bangladesh

2020 ◽  
Author(s):  
Chantelle Hooper ◽  
Partho P. Debnath ◽  
Sukumar Biswas ◽  
Ronny van Aerle ◽  
Kelly S. Bateman ◽  
...  
Keyword(s):  
Virus Assembly ◽  
Rna Virus ◽  
Macrobrachium Rosenbergii ◽  
Freshwater Prawn ◽  
The Novel ◽  
Protein Motifs ◽  
Giant Freshwater Prawn ◽  
Yellow Head Virus ◽  
Novel Virus ◽  
Mass Mortalities

1AbstractMass mortalities of the larval stage of the giant freshwater prawn, Macrobrachium rosenbergii, have been occurring in Bangladesh since 2011. Mortalities can reach 100% and have resulted in an 80% decline in the number of hatcheries actively producing M. rosenbergii. To investigate a causative agent for the mortalities, a disease challenge was carried out using infected material from a hatchery experiencing mortalities. Moribund larvae from the challenge were prepared for metatranscriptomic sequencing. De novo virus assembly revealed a 29 kb single-stranded positive-sense RNA virus with similarities in key protein motif sequences to yellow head virus (YHV), an RNA virus that causes mass mortalities in marine shrimp aquaculture, and other viruses in the Nidovirales order. Primers were designed against the novel virus and used to screen cDNA from larvae sampled from hatcheries in the South of Bangladesh from two consecutive years. Larvae from all hatcheries screened from both years were positive by PCR for the novel virus, including larvae from a hatchery that at the point of sampling appeared healthy, but later experienced mortalities. These screens suggest that the virus is widespread in M. rosenbergii hatchery culture in southern Bangladesh, and that early detection of the virus can be achieved by PCR. The hypothesised protein motifs of MrGV suggest that it is likely to be a new species within the Nidovirales order. Biosecurity measures should be taken in order to mitigate global spread through the movement of post-larvae within and between countries, which has previously been linked to other virus outbreaks in crustacean aquaculture.

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