scholarly journals Comparison of the enzyme-linked immunosorbent assay (ELISA) and the fluorescent antibody test (FAT) for measuring the prevalences and levels of Renibacterium salmoninarum in wild and hatchery stocks of salmonid fishes in Alaska. USA

1993 ◽  
Vol 16 ◽  
pp. 181-189 ◽  
Author(s):  
TR Meyers ◽  
S Short ◽  
C Farrington ◽  
K Lipson ◽  
HJ Geiger ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Salmonid Fishes ◽  
Renibacterium Salmoninarum

scholarly journals Comparison of the Membrane-Filtration Fluorescent Antibody Test, the Enzyme-Linked Immunosorbent Assay, and the Polymerase Chain Reaction to Detect Renibacterium Salmoninarum in Salmonid Ovarian Fluid

1998 ◽  
Vol 10 (1) ◽  
pp. 60-66 ◽  
Author(s):  
Ronald J. Pascho ◽  
Dorothy Chase ◽  
Connie L. McKibben
Keyword(s):  
Polymerase Chain Reaction ◽  
Immunosorbent Assay ◽  
Membrane Filtration ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ovarian Fluid ◽  
Chain Reaction ◽  
Renibacterium Salmoninarum ◽  
Polymerase Chain

Ovarian fluid samples from naturally infected chinook salmon ( Oncorhynchus tshawytscha) were examined for the presence of Renibacterium salmoninarum by the membrane-filtration fluorescent antibody test (MF-FAT), an antigen capture enzyme-linked immunosorbent assay (ELISA), and a nested polymerase chain reaction (PCR). On the basis of the MF-FAT, 64% (66/103) samples contained detectable levels of R. salmoninarum cells. Among the positive fish, the R. salmoninarum concentrations ranged from 25 cells/ml to 4.3 × 109 cells/ml. A soluble antigenic fraction of R. salmoninarum was detected in 39% of the fish (40/103) by the ELISA. The ELISA is considered one of the most sensitive detection methods for bacterial kidney disease in tissues, yet it did not detect R. salmoninarum antigen consistently at bacterial cell concentrations below about 1.3 × 104 cells/ml according to the MF-FAT counts. When total DNA was extracted and tested in a nested PCR designed to amplify a 320-base-pair region of the gene encoding a soluble 57-kD protein of R. salmoninarum, 100% of the 100 samples tested were positive. The results provided strong evidence that R. salmoninarum may be present in ovarian fluids thought to be free of the bacterium on the basis of standard diagnostic methods.

scholarly journals Comparison of Enzyme-Linked Immunosorbent Assay, Indirect Fluorescent Antibody Test, and Direct Agglutination Test for Detecting Toxoplasma Gondii Antibodies in Naturally Aborted Ovine Fetuses

1989 ◽  
Vol 1 (2) ◽  
pp. 124-127 ◽  
Author(s):  
Sheryl L. Seefeldt ◽  
Clyde A. Kirkbride ◽  
Jitender P. Dubey
Keyword(s):  
Toxoplasma Gondii ◽  
Immunosorbent Assay ◽  
Indirect Fluorescent Antibody Test ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Agglutination Test ◽  
Enzyme Linked Immunosorbent Assay ◽  
Direct Agglutination Test ◽  
Indirect Fluorescent Antibody ◽  
Special Equipment

Results obtained in an enzyme-linked immunosorbent assay (ELISA), an indirect fluorescent antibody test (IFA), and a modified direct agglutination test (MAT) for Toxoplasma gondii antibodies from examination of fetal fluids from 377 aborted ovine fetuses were compared. Sixty-seven samples were positive by MAT (titers 1:16 to > 1:65,536), 58 were positive by ELISA, and 62 were positive by immunoglobulin G-IFA. The MAT was preferred because it required less time, labor, and special equipment. It was simple to run, could be done on serum from any species without modification, and it was more effective than the IFA for detecting toxoplasma antibodies in severely autolyzed fetuses. No advantage was found in determining immunoglobulin M antibodies in ovine fetal sera.

Antigenic relationship of N2-fixing Pseudomonas strain H8 to various known cultures and rice rhizosphere isolates studied by indirect enzyme-linked immunosorbent assay (ELISA)

1986 ◽  
Vol 32 (5) ◽  
pp. 402-408 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
H. Q. Yao ◽  
I. Watanabe
Keyword(s):  
Immunosorbent Assay ◽  
Fluorescent Antibody ◽  
Antibody Test ◽  
Rhizosphere Bacteria ◽  
Enzyme Linked Immunosorbent Assay ◽  
Acetylene Reduction Activity ◽  
Antigenic Relationship ◽  
Pseudomonas Sp ◽  
Reduction Activity ◽  
Relationship Of

The serological relationship of several, mainly Gram-negative, bacteria including type cultures and previously isolated rhizosphere bacteria, to Pseudomonas sp. strain H8, a N2-fixing rhizosphere isolate from Maahas soil, was examined by indirect enzyme-linked immunosorbent assay. None cross-reacted significantly with H8 antibody except some of those rhizosphere bacteria previously found cross-reactive by fluorescent antibody test. Several new rhizosphere isolates from wetland rice grown in various fields showed significant immunoreactivity, and most of the reactive bacteria reduced acetylene. However, the cultural characteristics and acetylene reduction activity of most of these isolates were different from those of H8. None of the rhizosphere isolates from dryland rice cross-reacted. In a pot experiment, bacterial cultures similar to H8 were found only in Maahas soil, but none reacted with antibodies against strains H8 and KLH76 (another N2-fixing pseudomonad from rice root). Other isolates showing reaction with these antibodies were, however, neither culturally similar to these strains nor N2 fixing. The data suggest that Pseudomonas sp. H8 is not distributed widely in soils other than that from which it was originally isolated.

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