scholarly journals Effects of Caribbean sponge secondary metabolites on bacterial surface colonization

2005 ◽  
Vol 40 ◽  
pp. 191-203 ◽  
Author(s):  
SR Kelly ◽  
E Garo ◽  
PR Jensen ◽  
W Fenical ◽  
JR Pawlik
Keyword(s):  
Secondary Metabolites ◽  
Surface Colonization ◽  
Bacterial Surface

scholarly journals Tad pili play a dynamic role in Caulobacter crescentus surface colonization

2019 ◽  
Author(s):  
Matteo Sangermani ◽  
Isabelle Hug ◽  
Nora Sauter ◽  
Thomas Pfohl ◽  
Urs Jenal
Keyword(s):  
Caulobacter Crescentus ◽  
Optical Trap ◽  
Natural Environments ◽  
Surface Attachment ◽  
Surface Colonization ◽  
Bacterial Surface ◽  
Controlled Flow ◽  
Mechanical Sensing

ABSTRACTBacterial surface attachment is mediated by rotary flagella and filamentous appendages called pili. Here, we describe the role of Tad pili during surface colonization of Caulobacter crescentus. Using an optical trap and microfluidic controlled flow conditions as a mimic of natural environments, we demonstrate that Tad pili undergo repeated cycles of extension and retraction. Within seconds after establishing surface contact, pili reorient cells into an upright position promoting walking-like movements against the medium flow. Pili-mediated positioning of the flagellated pole close to the surface facilitates motor-mediated mechanical sensing and promotes anchoring of the holdfast, an adhesive substance that affords long-term attachment. We present evidence that the second messenger c-di-GMP regulates pili dynamics during surface encounter in distinct ways, promoting increased activity at intermediate levels and retraction of pili at peak concentrations. We propose a model, in which flagellum and Tad pili functionally interact and together impose a ratchet-like mechanism that progressively drives C. crescentus cells towards permanent surface attachment.

scholarly journals Cross-Ocean Distribution of Rhodobacterales Bacteria as Primary Surface Colonizers in Temperate Coastal Marine Waters

2007 ◽  
Vol 74 (1) ◽  
pp. 52-60 ◽  
Author(s):  
Hongyue Dang ◽  
Tiegang Li ◽  
Mingna Chen ◽  
Guiqiao Huang
Keyword(s):  
Atlantic Coast ◽  
Adaptation Strategy ◽  
Current Data ◽  
Control Surface ◽  
Rrna Gene ◽  
Multivariate Statistical ◽  
Surface Colonization ◽  
Bacterial Surface ◽  
West Pacific Ocean ◽  
Bacterial Assemblages

ABSTRACT Bacterial surface colonization is a universal adaptation strategy in aquatic environments. However, neither the identities of early colonizers nor the temporal changes in surface assemblages are well understood. To determine the identities of the most common bacterial primary colonizers and to assess the succession process, if any, of the bacterial assemblages during early stages of surface colonization in coastal water of the West Pacific Ocean, nonnutritive inert materials (glass, Plexiglas, and polyvinyl chloride) were employed as test surfaces and incubated in seawater off the Qingdao coast in the spring of 2005 for 24 and 72 h. Phylogenetic analysis of the 16S rRNA gene sequences amplified from the recovered surface-colonizing microbiota indicated that diverse bacteria colonized the submerged surfaces. Multivariate statistical cluster analyses indicated that the succession of early surface-colonizing bacterial assemblages followed sequential steps on all types of test surfaces. The Rhodobacterales, especially the marine Roseobacter clade members, formed the most common and dominant primary surface-colonizing bacterial group. Our current data, along with previous studies of the Atlantic coast, indicate that the Rhodobacterales bacteria are the dominant and ubiquitous primary surface colonizers in temperate coastal waters of the world and that microbial surface colonization follows a succession sequence. A conceptual model is proposed based on these findings, which may have important implications for understanding the structure, dynamics, and function of marine biofilms and for developing strategies to harness or control surface-associated microbial communities.

scholarly journals Characterization of initial events in bacterial surface colonization by twoPseudomonas species using image analysis

1992 ◽  
Vol 39 (11) ◽  
pp. 1161-1170 ◽  
Author(s):  
Robert F. Mueller ◽  
William G. Characklis ◽  
Warren L. Jones ◽  
John T. Sears
Keyword(s):  
Image Analysis ◽  
Surface Colonization ◽  
Bacterial Surface

scholarly journals Bacterial Surface Colonization of Sputter-Coated Platinum Films

Materials ◽  
2020 ◽  
Vol 13 (12) ◽  
pp. 2674
Author(s):  
Dominika Czerwińska-Główka ◽  
Wioletta Przystaś ◽  
Ewa Zabłocka-Godlewska ◽  
Sebastian Student ◽  
Beata Cwalina ◽  
...  
Keyword(s):  
Thin Layer ◽  
Orthopedic Implants ◽  
Bacterial Biofilm ◽  
Surgical Instruments ◽  
Bacterial Cells ◽  
Biomedical Devices ◽  
Surface Colonization ◽  
Bacterial Surface ◽  
Coated Glass ◽  
Short Time

Due to its biocompatibility and advantageous electrochemical properties, platinum is commonly used in the design of biomedical devices, e.g., surgical instruments, as well as electro-medical or orthopedic implants. This article verifies the hypothesis that a thin layer of sputter-coated platinum may possess antibacterial effects. The purpose of this research was to investigate the adhesion and growth ability of a model strain of Gram-negative bacteria, Escherichia coli, on a surface of a platinum-coated glass slide. Although some previous literature reports suggests that a thin layer of platinum would inhibit the formation of bacterial biofilm, the results of this study suggest otherwise. The decrease in the number of bacterial cells attached to the platinum-coated glass, which was observed within first three hours of culturing, was found to be a short-time effect, vanishing after 24 h. Consequently, it was shown that a thin layer of sputter-coated platinum did not exhibit any antibacterial effect. For this reason, this study indicates an urgent need for the development of new methods of surface modification that could reduce bacterial surface colonization of platinum-based biomedical devices.

scholarly journals Weak Rolling Adhesion Enhances Bacterial Surface Colonization

2006 ◽  
Vol 189 (5) ◽  
pp. 1794-1802 ◽  
Author(s):  
Brett N. Anderson ◽  
Albert M. Ding ◽  
Lina M. Nilsson ◽  
Kaoru Kusuma ◽  
Veronika Tchesnokova ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Single Point ◽  
Point Mutations ◽  
Stationary Mode ◽  
Surface Colonization ◽  
Bacterial Surface ◽  
Implanted Medical Devices ◽  
The Many ◽  
Rolling Mode ◽  
Strength Of Adhesion

ABSTRACT Bacterial adhesion to and subsequent colonization of surfaces are the first steps toward forming biofilms, which are a major concern for implanted medical devices and in many diseases. It has generally been assumed that strong irreversible adhesion is a necessary step for biofilm formation. However, some bacteria, such as Escherichia coli when binding to mannosylated surfaces via the adhesive protein FimH, adhere weakly in a mode that allows them to roll across the surface. Since single-point mutations or even increased shear stress can switch this FimH-mediated adhesion to a strong stationary mode, the FimH system offers a unique opportunity to investigate the role of the strength of adhesion independently from the many other factors that may affect surface colonization. Here we compare levels of surface colonization by E. coli strains that differ in the strength of adhesion as a result of flow conditions or point mutations in FimH. We show that the weak rolling mode of surface adhesion can allow a more rapid spreading during growth on a surface in the presence of fluid flow. Indeed, an attempt to inhibit the adhesion of strongly adherent bacteria by blocking mannose receptors with a soluble inhibitor actually increased the rate of surface colonization by allowing the bacteria to roll. This work suggests that (i) a physiological advantage to the weak adhesion demonstrated by commensal variants of FimH bacteria may be to allow rapid surface colonization and (ii) antiadhesive therapies intended to prevent biofilm formation can have the unintended effect of enhancing the rate of surface colonization.

scholarly journals Chemical mediation of bacterial surface colonisation by secondary metabolites from the red alga Delisea pulchra

1998 ◽  
Vol 15 ◽  
pp. 233-246 ◽  
Author(s):  
R Maximilien ◽  
R de Nys ◽  
C Holmström ◽  
L Gram ◽  
M Givskov ◽  
...  
Keyword(s):  
Secondary Metabolites ◽  
Red Alga ◽  
Bacterial Surface ◽  
Chemical Mediation ◽  
Delisea Pulchra

scholarly journals Tad Pili Play a Dynamic Role in Caulobacter crescentus Surface Colonization

mBio ◽  
2019 ◽  
Vol 10 (3) ◽  
Author(s):  
Matteo Sangermani ◽  
Isabelle Hug ◽  
Nora Sauter ◽  
Thomas Pfohl ◽  
Urs Jenal
Keyword(s):  
Caulobacter Crescentus ◽  
Optical Trap ◽  
Model Organism ◽  
Natural Environments ◽  
Surface Attachment ◽  
Content Type ◽  
Surface Colonization ◽  
Bacterial Surface ◽  
Solid Media ◽  
Resilient Communities

ABSTRACT Bacterial surface attachment is mediated by filamentous appendages called pili. Here, we describe the role of Tad pili during surface colonization of Caulobacter crescentus. Using an optical trap and microfluidic controlled flow conditions to mimic natural environments, we demonstrated that Tad pili undergo repeated dynamic cycles of extension and retraction. Within seconds after establishing surface contact, pilus retraction reorients cells into an upright position, promoting walking-like movements against the medium flow. Pilus-mediated positioning of the flagellate pole close to the surface facilitates motor-mediated mechanical sensing and promotes anchoring of the holdfast, an adhesive substance that affords long-term attachment. We present evidence that the second messenger c-di-GMP regulates pilus dynamics during surface encounter in distinct ways, promoting increased activity at intermediate levels and retraction of pili at peak concentrations. We propose a model in which flagellum and Tad pili functionally interact and together impose a ratchet-like mechanism that progressively drives C. crescentus cells toward permanent surface attachment. IMPORTANCE Bacteria are able to colonize surfaces in environmental, industrial, and medical settings, where they form resilient communities called biofilms. In order to control bacterial surface colonization, microbiologists need to gain a detailed understanding of the processes that bacteria use to live at the liquid-surface interface and that allow them to adhere to and move on surfaces and eventually grow and persist on solid media. To facilitate these processes, bacteria are equipped with adhesive structures such as flagella and pili and with matrix components such as exopolysaccharides. How these cellular organelles are coordinated to optimize surface processes is currently subject to intense investigations. Here we used the model organism Caulobacter crescentus to demonstrate that polar pili are highly dynamic structures that are functionally interconnected with the flagellar motor to mediate surface sensing, thereby enforcing rapid and permanent surface attachment. These studies provide an entry point for an in-depth molecular analysis of bacterial surface colonization.

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