A member of the Rhodobacteraceae promotes initial biofilm formation via the secretion of extracellular factor(s)

2015 ◽  
Vol 75 (2) ◽  
pp. 155-167 ◽  
Author(s):  
I Kviatkovski ◽  
D Minz
Keyword(s):  
Biofilm Formation ◽  
Extracellular Factor ◽  
Initial Biofilm

scholarly journals Motility activity, slime production, biofilm formation and genetic typing by ERIC-PCR for Pseudomonas aeruginosa strains isolated from bovine and other sources (human and environment)

2014 ◽  
Vol 17 (2) ◽  
pp. 321-329 ◽  
Author(s):  
K. Wolska ◽  
P. Szweda ◽  
K. Lada ◽  
E. Rytel ◽  
K. Gucwa ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
Bovine Mastitis ◽  
Twitching Motility ◽  
Phenotypic Properties ◽  
Biofilm Production ◽  
Abiotic Surfaces ◽  
Hospital Patients ◽  
Relationship Of ◽  
Initial Biofilm ◽  
Different Sources

AbstractThe molecular-typing strategy, ERIC-PCR was used in an attempt to determine the genomic relationship of 28 P. aeruginosa strains isolated from faeces of healthy bovine, bovine mastitis and from faeces of hospital patients as well as from environment. ERIC-PCR fingerprinting revealed large molecular differentiation within this group of isolates. Twenty two out of 28 strains tested generated unique patterns of DNA bands and only three genotypes consisted of two isolates each were identified. We also tested the P. aeruginosa isolates for their ability to form a biofilm on abiotic surfaces including polyvinylchloride and polystyrene. Different biofilm-forming abilities were demonstrated among strains; however, most of them (64.3%) showed moderate-biofilm forming ability. The strains with increased swimming and twitching motility displayed elevated biofilm formation. However, a negative correlation was found between slime and initial biofilm production. On the basis of the results obtained, we suggest that there are no major differences in phenotypic properties between P. aeruginosa strains isolated from different sources

Influence of Glucose Concentrations on Biofilm Formation, Motility, Exoprotease Production, and Quorum Sensing in Aeromonas hydrophila

2013 ◽  
Vol 76 (2) ◽  
pp. 239-247 ◽  
Author(s):  
IQBAL KABIR JAHID ◽  
NA-YOUNG LEE ◽  
ANNA KIM ◽  
SANG-DO HA
Keyword(s):  
Quorum Sensing ◽  
Aeromonas Hydrophila ◽  
Biofilm Formation ◽  
Opportunistic Pathogen ◽  
Homoserine Lactone ◽  
Protease Production ◽  
Acyl Homoserine Lactone ◽  
Human Pathogen ◽  
Initial Biofilm ◽  
Motility Inhibition

Aeromonas hydrophila recently has received increased attention because it is opportunistic and a primary human pathogen. A. hydrophila biofilm formation and its control are a major concern for food safety because biofilms are related to virulence. Therefore, we investigated biofilm formation, motility inhibition, quorum sensing, and exoprotease production of this opportunistic pathogen in response to various glucose concentrations from 0.05 to 2.5% (wt/vol). More than 0.05% glucose significantly impaired (P < 0.05) quorum sensing, biofilm formation, protease production, and swarming and swimming motility, whereas bacteria treated with 0.05% glucose had activity similar to that of the control (0% glucose). A stage shift biofilm assay revealed that the addition of glucose (2.5%) inhibited initial biofilm formation but not later stages. However, addition of quorum sensing molecules N-3-butanoyl-DL-homoserine lactone and N-3-hexanoyl homoserine lactone partially restored protease production, indicating that quorum sensing is controlled by glucose concentrations. Thus, glucose present in food or added as a preservative could regulate acyl-homoserine lactone quorum sensing molecules, which mediate biofilm formation and virulence in A. hydrophila.

Modified Implant Surface with Slower and Less Initial Biofilm Formation

2013 ◽  
Vol 17 (3) ◽  
pp. 461-468 ◽  
Author(s):  
Gordon John ◽  
Jürgen Becker ◽  
Frank Schwarz
Keyword(s):  
Biofilm Formation ◽  
Implant Surface ◽  
Initial Biofilm

Prevention of Initial Biofilm Formation on Ureteral Stents Using a Sustained Releasing Varnish Containing Chlorhexidine: In Vitro Study

2013 ◽  
Vol 27 (3) ◽  
pp. 333-337 ◽  
Author(s):  
Genady Zelichenko ◽  
Doron Steinberg ◽  
Gideon Lorber ◽  
Michael Friedman ◽  
Batia Zaks ◽  
...  
Keyword(s):  
Biofilm Formation ◽  
In Vitro Study ◽  
Vitro Study ◽  
Ureteral Stents ◽  
Initial Biofilm

scholarly journals Indonesian Strain of Lactobacillus reuteri Probiotic Reduces the Initial Biofilm Colonization

2020 ◽  
Vol 14 (1) ◽  
pp. 544-553
Author(s):  
Armelia Sari Widyarman ◽  
Triska Ramajayanti ◽  
Citra Fragrantia Theodorea
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Lactobacillus Reuteri ◽  
Positive Control ◽  
Negative Control ◽  
Microbial Biofilms ◽  
Incubation Periods ◽  
Negative Controls ◽  
Initial Biofilm

Background: The benefits of probiotics for human health have long been proven. Probiotic Lactobacillus reuteri, can produce a beneficial broad-spectrum antibacterial compound called reuterin by metabolizing glycerol. Objective: The aim of the study was to investigate the effect of the Indonesian strain of L. reuteri LC382415 on mono- and dual-species Streptococcus mutans and Streptococcus sanguinis biofilms in vitro. Methods: Streptococcus mutans and S. sanguinis were cultured in BHI broth. Lactobacillus reuteri LC382415 was inoculated on MRS agar. The different concentrations effect of L. reuteri (1×104, 1×106, and 1×108 CFU/mL) with and without glycerol supplementation on microbial biofilms were examined using a biofilm assay after incubation for 1,3,6, and 24-h. The biofilm mass optical density was measured with a microplate spectrophotometer at 490 nm. Chlorhexidine gluconate (0.2%) was used as a positive control, and wells without treatment were used as negative controls. Results: A significant reduction in mono- and dual-species S. mutans and S. sanguinis biofilm formation was observed after treatment with all concentrations of L.reuteri and after all incubation periods (p<0.05) with or without glycerol supplementation. The concentration of 1×104 CFU/mL after 3-h incubation was the most effective in inhibiting biofilm formation, with 87.8% S. mutans, 95.9% S. sanguinis, and 80.4% dual-species biofilm reduction compared to the negative control (p<0.05). Conclusion: The Indonesian strain of L. reuteri effectively reduces mono- and dual-species S.mutans and S. sanguinis biofilms. This suggests that it may be useful in preventing biofilm formation in oral cavities. Future studies on the mechanism of action of this active component are warranted.

scholarly journals Conditioning film and initial biofilm formation on ceramics tiles in the marine environment

2007 ◽  
Vol 274 (1) ◽  
pp. 24-29 ◽  
Author(s):  
Nachshon Siboni ◽  
Michal Lidor ◽  
Esti Kramarsky-Winter ◽  
Ariel Kushmaro
Keyword(s):  
Marine Environment ◽  
Biofilm Formation ◽  
Conditioning Film ◽  
Initial Biofilm

Conditioning film and initial biofilm formation on electrochemical CaCO3deposition on a metallic net in the marine environment

Biofouling ◽  
2009 ◽  
Vol 25 (7) ◽  
pp. 675-683 ◽  
Author(s):  
Nachshon Siboni ◽  
Stephane Martinez ◽  
Avigdor Abelson ◽  
Alex Sivan ◽  
Ariel Kushmaro
Keyword(s):  
Marine Environment ◽  
Biofilm Formation ◽  
Conditioning Film ◽  
Initial Biofilm

Role of the luxS Gene in Initial Biofilm Formation by Streptococcus mutans

2015 ◽  
Vol 25 (1) ◽  
pp. 60-68 ◽  
Author(s):  
Zhiyan He ◽  
Jingping Liang ◽  
Zisheng Tang ◽  
Rui Ma ◽  
Huasong Peng ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Mutant Strain ◽  
Biofilm Formation ◽  
Type Strain ◽  
Laser Scanning ◽  
Wild Type Strain ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Wild Type ◽  
Initial Biofilm

Quorum sensing (QS) is a process by which bacteria communicate with each other by secreting chemical signals called autoinducers (AIs). Among Gram-negative and Gram-positive bacteria, AI-2 synthesized by the LuxS enzyme is widespread. The aim of this study was to evaluate the effect of QS <i>luxS</i> gene on initial biofilm formation by <i>Streptococcus mutans</i>. The bacterial cell surface properties, including cell hydrophobicity (bacterial adherence to hydrocarbons) and aggregation, which are important for initial adherence during biofilm development, were investigated. The biofilm adhesion assay was evaluated by the MTT method. The structures of the 5-hour biofilms were observed by using confocal laser scanning microscopy, and QS-related gene expressions were investigated by real-time PCR. The <i>luxS</i> mutant strain exhibited higher biofilm adherence and aggregation, but lower hydrophobicity than the wild-type strain. The confocal laser scanning microscopy images revealed that the wild-type strain tended to form smaller aggregates with uniform distribution, whereas the <i>luxS</i> mutant strain aggregated into distinct clusters easily discernible in the generated biofilm. Most of the genes examined were downregulated in the biofilms formed by the <i>luxS</i> mutant strain, except the <i>gtfB </i>gene. QS <i>luxS</i> gene can affect the initial biofilm formation by <i>S. mutans.</i>

Efficacy of metal ions and isothiazolones in inhibiting Enterobacter cloacae BF-17 biofilm formation

2014 ◽  
Vol 60 (1) ◽  
pp. 5-14 ◽  
Author(s):  
Gang Zhou ◽  
Long-jie Li ◽  
Qing-shan Shi ◽  
You-sheng Ouyang ◽  
Yi-ben Chen ◽  
...  
Keyword(s):  
Metal Ions ◽  
Biofilm Formation ◽  
High Capacity ◽  
Enterobacter Cloacae ◽  
High Concentration ◽  
Planktonic Growth ◽  
Low Concentrations ◽  
Negative Effect ◽  
Biofilm Development ◽  
Initial Biofilm

Enterobacter cloacae is a nosocomial pathogen. The E. cloacae strain BF-17, with a high capacity for biofilm formation, was screened and identified from industrially contaminated samples, carried out in our laboratory. To develop an efficient strategy to deal with biofilms, we investigated the effects of metal ions, including Na+, K+, Ca2+, Mg2+, Cu2+, and Mn2+, and 3 isothiazolones, on elimination of E. cloacae BF-17 biofilm formation by using a 0.1% crystal violet staining method. The results revealed that higher concentrations of Na+ or K+ significantly inhibited E. cloacae BF-17 biofilm development. Meanwhile, Ca2+ and Mn2+ stimulated biofilm formation at low concentration but exhibited a negative effect at high concentration. Moreover, biofilm formation decreased with increasing concentration of Mg2+ and Cu2+. The isothiazolones Kathon (14%), 1,2-benzisothiazolin-3-one (11%), and 2-methyl-4-isothiazolin-3-one (10%) stimulated initial biofilm formation but not planktonic growth at low concentrations and displayed inhibitory effects on both biofilm formation and planktonic growth at higher concentrations. Unfortunately, the 3 isothiazolones exerted negligible effects on preformed or fully mature biofilms. Our findings suggest that Na+, K+, Mg2+, and isothiazolones could be used to prevent and eliminate E. cloacae BF-17 biofilms.

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