scholarly journals Clone library analysis reveals an unusual composition and strong habitat partitioning of pelagic bacterial communities in Lake Tanganyika

2008 ◽  
Vol 50 ◽  
pp. 113-122 ◽  
Author(s):  
A De Wever ◽  
K Van der Gucht ◽  
K Muylaert ◽  
S Cousin ◽  
W Vyverman
Keyword(s):  
Clone Library ◽  
Bacterial Communities ◽  
Lake Tanganyika ◽  
Habitat Partitioning ◽  
Clone Library Analysis

scholarly journals Succession of Bacterial Community Structure along the Changjiang River Determined by Denaturing Gradient Gel Electrophoresis and Clone Library Analysis

2002 ◽  
Vol 68 (10) ◽  
pp. 5142-5150 ◽  
Author(s):  
Hiroyuki Sekiguchi ◽  
Masataka Watanabe ◽  
Tadaatsu Nakahara ◽  
Baohua Xu ◽  
Hiroo Uchiyama
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Clone Library ◽  
Bacterial Communities ◽  
Bacterial Community Structure ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Changjiang River ◽  
The Changjiang River ◽  
Clone Library Analysis ◽  
Gradient Gel Electrophoresis

ABSTRACT Bacterial community structure along the Changjiang River (which is more than 2,500 km long) was studied by using denaturing gradient gel electrophoresis (DGGE) and clone library analysis of PCR-amplified 16S ribosomal DNA (rDNA) with universal bacterial primer sets. DGGE profiles and principal-component analysis (PCA) demonstrated that the bacterial community gradually changed from upstream to downstream in both 1998 and 1999. Bacterial diversity, as determined by the Shannon index (H′), gradually decreased from upstream to downstream. The PCA plots revealed that the differences in the bacterial communities among riverine stations were not appreciable compared with the differences in two adjacent lakes, Lake Dongting and Lake Poyang. The relative stability of the bacterial communities at the riverine stations was probably due to the buffering action of the large amount of water flowing down the river. Clone library analysis of 16S rDNA revealed that the dominant bacterial groups changed from β-proteobacteria and the Cytophaga-Flexibacter-Bacteroides group upstream to high-G+C-content gram-positive bacteria downstream and also that the bacterial community structure differed among the stations in the river and the lakes. The results obtained in this study should provide a reference for future changes caused by construction of the Three Gorges Dam.

scholarly journals Evidence for Vertical Transmission of Bacterial Symbionts from Adult to Embryo in the Caribbean Sponge Svenzea zeai

2009 ◽  
Vol 75 (19) ◽  
pp. 6147-6156 ◽  
Author(s):  
On On Lee ◽  
Pui Yi Chui ◽  
Yue Him Wong ◽  
Joseph R. Pawlik ◽  
Pei-Yuan Qian
Keyword(s):  
Water Column ◽  
Clone Library ◽  
Bacterial Communities ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Rrna Gene ◽  
Vertical Transfer ◽  
Clone Library Analysis ◽  
Gradient Gel Electrophoresis ◽  
The Caribbean ◽  
Surrounding Seawater

ABSTRACT The Caribbean reef sponge Svenzea zeai was previously found to contain substantial quantities of unicellular photosynthetic and autotrophic microbes in its tissues, but the identities of these symbionts and their method of transfer from adult to progeny are largely unknown. In this study, both a 16S rRNA gene-based fingerprinting technique (denaturing gradient gel electrophoresis [DGGE]) and clone library analysis were applied to compare the bacterial communities associated with adults and embryos of S. zeai to test the hypothesis of vertical transfer across generations. In addition, the same techniques were applied to the bacterial community from the seawater adjacent to adult sponges to test the hypothesis that water column bacteria could be transferred horizontally as sponge symbionts. Results of both DGGE and clone library analysis support the vertical transfer hypothesis in that the bacterial communities associated with sponge adults and embryos were highly similar to each other but completely different from those in the surrounding seawater. Sequencing of prominent DGGE bands and of clones from the libraries revealed that the bacterial communities associated with the sponge, whether adult or embryo, consisted of a large proportion of bacteria in the phyla Chloroflexi and Acidobacteria, while most of the sequences recovered from the community in the adjacent water column belonged to the class Alphaproteobacteria. Altogether, 21 monophyletic sequence clusters, comprising sequences from both sponge adults and embryos but not from the seawater, were identified. More than half of the sponge-derived sequences fell into these clusters. Comparison of sequences recovered in this study with those deposited in GenBank revealed that more than 75% of S. zeai-derived sequences were closely related to sequences derived from other sponge species, but none of the sequences recovered from the seawater column overlapped with those from adults or embryos of S. zeai. In conclusion, there is strong evidence that a dominant proportion of sponge-specific bacteria present in the tissues of S. zeai are maintained through vertical transfer during embryogenesis rather than through acquisition from the environment (horizontal transfer).

scholarly journals Microbial community in persistent apical periodontitis: a 16S rRNA gene clone library analysis

2014 ◽  
Vol 48 (8) ◽  
pp. 717-728 ◽  
Author(s):  
M. N. Zakaria ◽  
T. Takeshita ◽  
Y. Shibata ◽  
H. Maeda ◽  
N. Wada ◽  
...  
Keyword(s):  
Microbial Community ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Clone Library ◽  
Apical Periodontitis ◽  
Gene Clone ◽  
Rrna Gene ◽  
Clone Library Analysis

A diverse bacterial community in an anoxic quinoline-degrading bioreactor determined by using pyrosequencing and clone library analysis

2011 ◽  
Vol 91 (2) ◽  
pp. 425-434 ◽  
Author(s):  
Xiaojun Zhang ◽  
Siqing Yue ◽  
Huihui Zhong ◽  
Weiying Hua ◽  
Ruijia Chen ◽  
...  
Keyword(s):  
Bacterial Community ◽  
Clone Library ◽  
Clone Library Analysis

scholarly journals Achromobacter buckle infection diagnosed by a 16S rDNA clone library analysis: a case report

2014 ◽  
Vol 14 (1) ◽  
Author(s):  
Fumika Hotta ◽  
Hiroshi Eguchi ◽  
Takeshi Naito ◽  
Yoshinori Mitamura ◽  
Kohei Kusujima ◽  
...  
Keyword(s):  
Case Report ◽  
16S Rdna ◽  
Clone Library ◽  
16S Rdna Clone Library ◽  
Clone Library Analysis ◽  
Rdna Clone ◽  
Buckle Infection

The bacteriological incidence of lung abscess using clone library analysis of 16S rRNA gene in bronchoalveolar lavage

2015 ◽  
Author(s):  
Keisuke Naito ◽  
Shingo Noguchi ◽  
Kazuhiro Yatera ◽  
Kentarou Akata ◽  
Chinatsu Nishida ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Bronchoalveolar Lavage ◽  
Clone Library ◽  
Lung Abscess ◽  
Rrna Gene ◽  
Clone Library Analysis

Assessing genetic structure and diversity of airborne bacterial communities by DNA fingerprinting and 16S rDNA clone library

2005 ◽  
Vol 39 (20) ◽  
pp. 3687-3695 ◽  
Author(s):  
Pierre-Alain Maron ◽  
David P.H. Lejon ◽  
Esmeralda Carvalho ◽  
Karine Bizet ◽  
Philippe Lemanceau ◽  
...  
Keyword(s):  
Genetic Structure ◽  
16S Rdna ◽  
Dna Fingerprinting ◽  
Clone Library ◽  
Bacterial Communities ◽  
16S Rdna Clone Library ◽  
Genetic Structure And Diversity ◽  
Rdna Clone
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