scholarly journals Whole-mount fluorescence in situ hybridization to visualize symbiotic bacteria in the gills of deep‑sea mussels

2012 ◽  
Vol 14 (2) ◽  
pp. 135-140 ◽  
Author(s):  
M Fujinoki ◽  
T Koito ◽  
Y Fujiwara ◽  
M Kawato ◽  
Y Tada ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Deep Sea ◽  
Symbiotic Bacteria ◽  
Whole Mount

scholarly journals FISHing for chick genes: Triple-label whole-mount fluorescence in situ hybridization detects simultaneous and overlapping gene expression in avian embryos

2004 ◽  
Vol 229 (3) ◽  
pp. 651-657 ◽  
Author(s):  
Nathaniel Denkers ◽  
Pilar García-Villalba ◽  
Christopher K. Rodesch ◽  
Kandice R. Nielson ◽  
Teri Jo Mauch
Keyword(s):  
Gene Expression ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Avian Embryos ◽  
Overlapping Gene ◽  
Whole Mount

scholarly journals Fluorescence In Situ Hybridization in Whole-Mount Drosophila Embryos

BioTechniques ◽  
1996 ◽  
Vol 20 (5) ◽  
pp. 748-750 ◽  
Author(s):  
Sarah C. Hughes ◽  
Benedicte Saulier-Le Drean ◽  
Izhar Livne-Bar ◽  
Henry M. Krause
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Whole Mount ◽  
Drosophila Embryos

scholarly journals Procedure for whole mount fluorescence in situ hybridization of interphase nuclei on Arabidopsis thaliana

1994 ◽  
Vol 6 (1) ◽  
pp. 123-131 ◽  
Author(s):  
Serge Bauwens ◽  
Katerina Katsanis ◽  
Marc Van Montagu ◽  
Patrick Van Oostveldt ◽  
Gilbert Engler
Keyword(s):  
Arabidopsis Thaliana ◽  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Interphase Nuclei ◽  
Whole Mount

scholarly journals Processing Deep-Sea Particle-Rich Water Samples for Fluorescence In Situ Hybridization: Consideration of Storage Effects, Preservation, and Sonication

2004 ◽  
Vol 70 (1) ◽  
pp. 25-33 ◽  
Author(s):  
Phyllis Lam ◽  
James P. Cowen
Keyword(s):  
In Situ Hybridization ◽  
Fluorescence In Situ Hybridization ◽  
Water Samples ◽  
Deep Sea ◽  
Cell Counting ◽  
Epifluorescence Microscopy ◽  
Size Fractionation ◽  
Cell Abundance ◽  
Abundance Estimates

ABSTRACT Particles are often regarded as microniches of enhanced microbial production and activities in the pelagic ocean and are vehicles of vertical material transport from the euphotic zone to the deep sea. Fluorescence in situ hybridization (FISH) can be a useful tool to study the microbial community structures associated with these particles, and thus their ecological significance, yet an appropriate protocol for processing deep-sea particle-rich water samples is lacking. Some sample processing considerations are discussed in the present study, and different combinations of existing procedures for preservation, size fractionation sequential filtration, and sonication were tested in conjunction with FISH. Results from this study show that water samples should be filtered and processed within no more than 10 to 12 h after collection, or else preservation is necessary. The commonly used prefiltration formaldehyde fixation was shown to be inadequate for the rRNA targeted by FISH. However, prefiltration formaldehyde fixation followed by immediate freezing and postfiltration paraformaldehyde fixation yielded highly consistent cell abundance estimates even after 96 days or potentially longer storage. Size fractionation sequential filtration and sonication together enhanced cell abundance estimates by severalfold. Size fractionation sequential filtration effectively separated particle-associated microbial communities from their free-living counterparts, while sonication detached cells from particles or aggregates for more-accurate cell counting using epifluorescence microscopy. Optimization in sonication time is recommended for different specific types of samples. These tested and optimized procedures can be incorporated into a FISH protocol for sampling in deep-sea particle-rich waters.

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