scholarly journals Isolation of seaweed-associated bacteria and their morphogenesis-inducing capability in axenic cultures of the green alga Ulva fasciata

2011 ◽  
Vol 12 (1) ◽  
pp. 13-21 ◽  
Author(s):  
RP Singh ◽  
VA Mantri ◽  
CRK Reddy ◽  
B Jha
Keyword(s):  
Green Alga ◽  
Associated Bacteria ◽  
Ulva Fasciata ◽  
Axenic Cultures

scholarly journals In vivo Release of Lectins from the Green Alga Ulva fasciata

2010 ◽  
Vol 5 (4) ◽  
pp. 1934578X1000500 ◽  
Author(s):  
Pablo Djabayan-Djibeyan ◽  
Roslyn Gibbs ◽  
Brian Carpenter
Keyword(s):  
Green Alga ◽  
Initial Period ◽  
Water Soluble ◽  
Uv Absorption ◽  
Selective Precipitation ◽  
Ulva Fasciata ◽  
Electrophoretic Mobilities ◽  
Value Transfer ◽  
Sample Count

The green alga Ulva fasciata Delile (Ulvaceae), after thawing from storage at −20oC, has been used to study the in vivo biosynthesis and release of lectins. The alga was made to resume viable growth by immersion in a PBS buffer, pH 7.4, containing 0.01% w/v sodium azide and irradiating with a halophosphate lamp. The growing alga readily took up 14C leucine, when this was added to the buffer, as seen by a decrease in a sample count rate of ~ 8000 cpm over a period of twenty minutes. The transfer of the radioactivity fed algae into fresh PBS buffer resulted in 14C labeled proteins being subsequently released into solution. As well as observing changes in levels of radioactivity, the release of proteins was also monitored by UV absorption at 280 nm. Both techniques indicated an initial steady release over the first twelve hours, followed by a slower approach to a plateau value. Transfer of the algae that had undergone an initial period of protein release into a subsequent second and third volume of fresh PBS buffer produced similar UV absorption profiles, but the total quantities of material released were reduced. Identification of the released proteins was obtained from their ability to agglutinate red blood cells, which was inhibited by L-fucose, and their electrophoretic mobilities when compared with earlier isolated samples of the U. fasciata lectin. The reference lectin was obtained by affinity chromatography, following the selective precipitation of the water soluble algal proteins with ammonium sulfate. We postulate that the observed release profiles support the previously suggested concept that lectins have the ability to function as protection agents for living marine algae.

A simple rapid procedure for obtaining axenic cultures from monoxenic cultures of myxomycete plasmodia

1999 ◽  
Vol 45 (10) ◽  
pp. 865-870 ◽  
Author(s):  
S Balaji ◽  
A Sujatha ◽  
I Kalyanasundaram
Keyword(s):  
Axenic Culture ◽  
Differential Staining ◽  
Complex Media ◽  
Associated Bacteria ◽  
Rapid Procedure ◽  
Substrate Preferences ◽  
Axenic Cultures ◽  
Time Required

Axenic culture of myxomycete plasmodia has been attempted from time to time by various authors, but with very little success. From over 500 known species of myxomycetes, fewer than 20 species have been reported in axenic culture to date, including axenic myxamoebal cultures. In these cultures, the plasmodia required either complex media, or a killed bacterial supplement for growth. Furthermore, the time required for attaining the axenic state varied from several months to years. In the present study, a simple, rapid procedure has been developed to render monoxenic plasmodial cultures axenic. This procedure is based on our discovery that plasmodia have certain unusual substrate preferences that are inhibitory to the associated bacteria using Physarella oblonga as a model. The presence or absence of the bacteria could be ascertained through incubation in four different bacteriological media and by the use of a differential staining technique.Key words: myxomycetes, axenic culture, hydrocarbon utilization, bacterial associates.

scholarly journals Bacteria enhance the production of extracellular polymeric substances by the green dinoflagellate Lepidodinium chlorophorum

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pauline Roux ◽  
Raffaele Siano ◽  
Karine Collin ◽  
Gwenael Bilien ◽  
Corinne Sinquin ◽  
...  
Keyword(s):  
Extracellular Polymeric Substances ◽  
Harmful Effect ◽  
Ne Atlantic ◽  
Associated Bacteria ◽  
Batch Cultures ◽  
Negative Effect ◽  
Axenic Cultures ◽  
High Concentrations ◽  
Axenic Conditions ◽  
Oyster Cultivation

AbstractHigh biomasses of the marine dinoflagellate Lepidodinium chlorophorum cause green seawater discolorations along Southern Brittany (NE Atlantic, France). The viscosity associated to these phenomena has been related to problems in oyster cultivation. The harmful effect of L. chlorophorum might originate from the secretion of Extracellular Polymeric Substances (EPS). To understand whether the EPS are produced by L. chlorophorum or its associated bacteria, or if they are a product of their interaction, batch cultures were performed under non-axenic and pseudo-axenic conditions for three strains. Maximum dinoflagellate cell abundances were observed in pseudo-axenic cultures. The non-sinking fraction of polymers (Soluble Extracellular Polymers, SEP), mainly composed of proteins and the exopolysaccharide sulphated galactan, slightly increased in pseudo-axenic cultures. The amount of Transparent Exopolymer Particles (TEP) per cell increased under non-axenic conditions. Despite the high concentrations of Particulate Organic Carbon (POC) measured, viscosity did not vary. These results suggest that the L. chlorophorum-bacteria interaction could have a detrimental consequence on the dinoflagellate, translating in a negative effect on L. chlorophorum growth, as well as EPS overproduction by the dinoflagellate, at concentrations that should not affect seawater viscosity.

scholarly journals Efficacy of marine green alga Ulva fasciata extract on the management of shrimp bacterial diseases

2011 ◽  
Vol 39 (2) ◽  
pp. 197-204 ◽  
Author(s):  
Joseph Selvin ◽  
Aseer Manilal ◽  
Suganthan Sujith ◽  
George Seghal Kiran ◽  
Aron Premnath Lipton
Keyword(s):  
Green Alga ◽  
Bacterial Diseases ◽  
Ulva Fasciata ◽  
Marine Green Alga

Optimization and Kinetic studies of biodiesel production from the green alga Ulva fasciata Delile

2019 ◽  
Vol 23 (4) ◽  
pp. 301-322
Author(s):  
Nayrah A. Shaltout ◽  
Mustafa A. Ghazal ◽  
Nihal G. Shams El-Din ◽  
Alaa E. Ali ◽  
Doha M. Beltagy
Keyword(s):  
Green Alga ◽  
Kinetic Studies ◽  
Biodiesel Production ◽  
Ulva Fasciata

Enzymatic hydrolysis and production of bioethanol from common macrophytic green alga Ulva fasciata Delile

2013 ◽  
Vol 150 ◽  
pp. 106-112 ◽  
Author(s):  
Nitin Trivedi ◽  
Vishal Gupta ◽  
C.R.K. Reddy ◽  
Bhavanath Jha
Keyword(s):  
Enzymatic Hydrolysis ◽  
Green Alga ◽  
Ulva Fasciata

An antiviral sphingosine derivative from the green alga Ulva Fasciata

1992 ◽  
Vol 33 (12) ◽  
pp. 1641-1644 ◽  
Author(s):  
Hari S. Garg ◽  
Mithlesh Sharma (nee' Pandey) ◽  
Dewan S. Bhakuni ◽  
Birenda N. Pramanik ◽  
Ajay K. Bose
Keyword(s):  
Green Alga ◽  
Ulva Fasciata

Three New Unsaturated Fatty Acids from the Marine Green Alga Ulva fasciata Delile

2009 ◽  
Vol 64 (10) ◽  
pp. 1199-1207 ◽  
Author(s):  
Ghada S. E. Abou-ElWafa ◽  
Mohamed Shaaban ◽  
Khaled A. Shaaban ◽  
Mohamed E. E. El-Naggar ◽  
Hartmut Laatsch
Keyword(s):  
Fatty Acids ◽  
Green Alga ◽  
Unsaturated Fatty Acids ◽  
Methyl Esters ◽  
2D Nmr ◽  
Mediterranean Coast ◽  
Enoic Acid ◽  
Ulva Fasciata ◽  
Brine Shrimps ◽  
Marine Green Alga

From the dichloromethane extract of the marine green alga Ulva fasciata Delile, collected from the Mediterranean coast of Egypt, three new fatty acids, namely, (E)-11-oxo-octadeca-12-enoic acid (1a), (E)-11-hydroxy-octadeca-12-enoic acid (2a) and 6-hydroxy-oct-7-enoic acid (3a) together with cholesterol were isolated. Analysis of the unpolar part of the extract using GC-MS detected the existence of further ten compounds, namely, dimethylsulfoxide, dimethylsulfone, phenylacetamide, 6,10,14-trimethyl-pentadecan-2-one, 8-heptadecene, dodecane, tridecane, 4-oxo-pentanoic acid, hexadecanoic acid, and the naturally new 1,1ʹ-bicyclohexyl. Structures of the isolated compounds 1a - 3a were confirmed by spectroscopic analyses including mass spectra (EI-MS, HR/ESIMS), 1D and 2D NMR experiments, and by the synthetic conversion into their corresponding methyl esters 1b - 3b. The algal extract and its components were comparatively examined against several pathogenic microorganisms, and brine shrimps for cytotoxicity.

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