scholarly journals Expression of Cell Proliferation-associated Nuclear Antigen (Ki-67) in Recurrent Aphthous Ulcers(RAU).

1998 ◽  
Vol 3 (1) ◽  
pp. 29-34 ◽  
Author(s):  
Sirajedin S. Natah ◽  
Jarkko Hietanen ◽  
Ritva Häyrinen-Immonen ◽  
Peter Jungell ◽  
Maria Malmström ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Nuclear Antigen ◽  
Ki 67 ◽  
Aphthous Ulcers ◽  
Recurrent Aphthous Ulcers

scholarly journals Antidepressant Effects of Probucol on Early-Symptomatic YAC128 Transgenic Mice for Huntington’s Disease

2018 ◽  
Vol 2018 ◽  
pp. 1-17 ◽  
Author(s):  
Cristine de Paula Nascimento-Castro ◽  
Ana Claudia Wink ◽  
Victor Silva da Fônseca ◽  
Claudia Daniele Bianco ◽  
Elisa C. Winkelmann-Duarte ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Huntington's Disease ◽  
Huntington’S Disease ◽  
Neuronal Differentiation ◽  
Neurodegenerative Disorder ◽  
Neuropsychiatric Symptoms ◽  
Chronic Administration ◽  
Nuclear Antigen ◽  
Ki 67 ◽  
Affective Symptoms

Huntington’s disease (HD) is an autosomal dominant neurodegenerative disorder caused by a trinucleotide expansion in the HD gene, resulting in an extended polyglutamine tract in the protein huntingtin. HD is traditionally viewed as a movement disorder, but cognitive and neuropsychiatric symptoms also contribute to the clinical presentation. Depression is one of the most common psychiatric disturbances in HD, present even before manifestation of motor symptoms. Diagnosis and treatment of depression in HD-affected individuals are essential aspects of clinical management in this population, especially owing to the high risk of suicide. This study investigated whether chronic administration of the antioxidant probucol improved motor and affective symptoms as well as hippocampal neurogenic function in the YAC128 transgenic mouse model of HD during the early- to mild-symptomatic stages of disease progression. The motor performance and affective symptoms were monitored using well-validated behavioral tests in YAC128 mice and age-matched wild-type littermates at 2, 4, and 6 months of age, after 1, 3, or 5 months of treatment with probucol (30 mg/kg/day via water supplementation, starting on postnatal day 30). Endogenous markers were used to assess the effect of probucol on cell proliferation (Ki-67 and proliferation cell nuclear antigen (PCNA)) and neuronal differentiation (doublecortin (DCX)) in the hippocampal dentate gyrus (DG). Chronic treatment with probucol reduced the occurrence of depressive-like behaviors in early- and mild-symptomatic YAC128 mice. Functional improvements were not accompanied by increased progenitor cell proliferation and neuronal differentiation. Our findings provide evidence that administration of probucol may be of clinical benefit in the management of early- to mild-symptomatic HD.

scholarly journals Proliferating cell nuclear antigen (PCNA) expressed in human leptomeninges.

1996 ◽  
Vol 44 (11) ◽  
pp. 1261-1265 ◽  
Author(s):  
H Funato ◽  
M Yoshimura ◽  
Y Ito ◽  
R Okeda ◽  
Y Ihara
Keyword(s):  
Monoclonal Antibody ◽  
Cell Proliferation ◽  
Smooth Muscle ◽  
Proliferating Cell Nuclear Antigen ◽  
Normal Subjects ◽  
Nuclear Antigen ◽  
Ependymal Cells ◽  
Ki 67 ◽  
Proliferating Cell ◽  
Strong Immunoreactivity

Here we report on the presence of proliferating cell nuclear antigen (PCNA) in human leptomeninges from 35 normal subjects with ages ranging from 57 to 94 years. Strong immunoreactivity with PC10 (a monoclonal antibody to PCNA) was detected in the nuclei of meningothelial cells, smooth muscle cells of leptomeningeal vessels, and ependymal cells. An immunoblot of leptomeningeal homogenate with PC10 showed the presence of a single band at 35 KD, the expected molecular mass of PCNA. Ki-67, another marker for cell proliferation, was undetectable in human leptomeninges. These observations point to isolated PCNA expression in tissue in which cells are not actively proliferating.

Expression of Bcl-2 and 3-ß hydroxysteroid dehydrogenase protein during oocyte and follicle development in fetal and post-natal pig ovaries

1999 ◽  
Vol 11 (8) ◽  
pp. 463 ◽  
Author(s):  
Wesley M. Garrett ◽  
H. David Guthrie
Keyword(s):  
Cell Proliferation ◽  
Germ Cell ◽  
Germ Cells ◽  
Granulosa Cells ◽  
Stromal Cells ◽  
Post Partum ◽  
Nuclear Antigen ◽  
Ki 67 ◽  
Primordial Follicles ◽  
Small Clusters

The fetal and post-natal development of the pig ovary involves both proliferation and apoptotic loss of germ cells, follicle formation and growth, and the initiation of oocyte meiotic maturation. The present study measured the expression of the proto-oncogene Bcl-2 immunohistochemically on paraffin sections of pig ovaries to determine its relationship with folliculogenesis on Days 50 and 80 post coitum (p.c.) and on Days 1, 21, and 56 post partum (p.p.). The expression of the steroidogenic enzyme 3β-hydroxy-steroid dehydrogenase (3βHSD) was used to determine the lineages of the cells forming the ovarian follicles, and the expression of the cell proliferation-associated nuclear antigen Ki-67 was used to determine germ cell proliferation and the initiation of follicle growth. Expression of Ki-67 showed that many oogonia were proliferating on Days 50 and 80 p.c. Granulosa cells were more proliferative on Day 56 p.p. than at any other stage; Ki-67 was expressed in 70% of growing follicles and granulosa cells had a 3% mean staining index per section. Less than 4% of germ cells and follicles had morphological signs of degeneration during the period of the study. Bcl-2 was most abundant on Days 21 p.p. and 56 p.p.; staining was localized to stromal cells among follicles and in small clusters in the cortical–medullary junction (CMJ). 3βHSD staining on Day 50 p.c. was seen in cords of stromal cells within the medulla of the ovary, and in the stromal cells investing the oogonial nests. On Days 80 p.c., 1 p.p., 21 p.p., and 56 p.p., 3βHSD was expressed in the granulosa cells of primary or primordial follicles at the CMJ. Production of Bcl-2 by somatic cells may support germ cell and preantral follicle survival.

scholarly journals Epitope analysis of antibodies recognising the cell proliferation associated nuclear antigen previously defined by the antibody Ki-67 (Ki-67 protein).

1994 ◽  
Vol 47 (6) ◽  
pp. 524-528 ◽  
Author(s):  
M H Kubbutat ◽  
G Key ◽  
M Duchrow ◽  
C Schluter ◽  
H D Flad ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Nuclear Antigen ◽  
Ki 67 ◽  
Epitope Analysis
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