scholarly journals Decreased C-reactive protein induces abnormal vascular structure in a rat model of liver dysfunction induced by bile duct ligation

2016 ◽  
Vol 22 (3) ◽  
pp. 372-381 ◽  
Author(s):  
Ji Hye Jun ◽  
Jong Ho Choi ◽  
Si Hyun Bae ◽  
Seh Hoon Oh ◽  
Gi Jin Kim
Keyword(s):  
Bile Duct ◽  
Rat Model ◽  
Liver Dysfunction ◽  
Bile Duct Ligation ◽  
Vascular Structure ◽  
C Reactive Protein ◽  
Reactive Protein ◽  
Duct Ligation

scholarly journals Human Chorionic Plate-Derived Mesenchymal Stem Cells Restore Hepatic Lipid Metabolism in a Rat Model of Bile Duct Ligation

2017 ◽  
Vol 2017 ◽  
pp. 1-9 ◽  
Author(s):  
Yun Bin Lee ◽  
Jong Ho Choi ◽  
Eun Nam Kim ◽  
Jin Seok ◽  
Hyun-Jung Lee ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Lipid Metabolism ◽  
Bile Duct ◽  
Rat Model ◽  
Liver Diseases ◽  
Bile Duct Ligation ◽  
Expression Levels ◽  
Duct Ligation ◽  
Chorionic Plate

In cholestatic liver diseases, impaired bile excretion disrupts lipid homeostasis. We investigated changes of lipid metabolism, including mitochondrial β-oxidation, in a rat model of bile duct ligation (BDL) in which chorionic plate-derived mesenchymal stem cells (CP-MSCs) were transplanted. Serum cholesterol level, which was elevated after BDL, was significantly decreased following CP-MSC transplantation. The expression levels of genes involved in intracellular lipid uptake, including long-chain fatty acyl-CoA synthetases and fatty acid transport proteins, were decreased in rats after BDL; however, they were not significantly changed by subsequent CP-MSC transplantation. Carnitine palmitoyltransferase 1A (CPT1A), a rate-limiting enzyme in mitochondrial β-oxidation, was upregulated after BDL and then was downregulated after CP-MSC transplantation. CPT1A expression was changed via microRNA-33—a posttranscriptional regulator of CPT1A—in a peroxisome proliferator-activated receptor α-independent manner. Cellular adenosine triphosphate production—an indicator of mitochondrial function—was reduced after BDL and was restored by CP-MSC transplantation. Expression levels of heme oxygenases also were significantly affected following BDL and CP-MSC transplantation. Lipid metabolism is altered in response to chronic cholestatic liver injury and can be restored by CP-MSC transplantation. Our study findings support the therapeutic potential of CP-MSCs in cholestatic liver diseases and help in understanding the fundamental mechanisms by which CP-MSCs affect energy metabolism.

scholarly journals Effect of Bile Duct Ligation-induced Liver Dysfunction on Methamphetamine Pharmacokinetics and Locomotor Activity in Rats

2019 ◽  
Vol 22 ◽  
pp. 301-312 ◽  
Author(s):  
Michael D Hambuchen ◽  
Michael D Berquist ◽  
Christy M Simecka ◽  
Mitchell R McGill ◽  
Melinda G Gunnell ◽  
...  
Keyword(s):  
Locomotor Activity ◽  
Bile Duct ◽  
Liver Dysfunction ◽  
Hepatic Dysfunction ◽  
Bile Duct Ligation ◽  
Dependent Manner ◽  
Duct Ligation ◽  
Male Wistar Rats ◽  
Experimental Treatments ◽  
Altered Liver

Purpose: Methamphetamine (METH) abuse is associated with hepatic dysfunction related comorbidities such as HIV, hepatitis C, and polysubstance abuse with acetaminophen-containing opioid formulations. We aimed to develop a bile duct ligation (BDL)-induced hepatic dysfunction model for studying both METH and experimental treatments for METH abuse in this comorbidity. Methods: Sham or BDL surgery was performed in male Wistar rats on day 0. Liver function was measured throughout the study. On days 7 and 19, serum pharmacokinetics studies were performed with 1 mg/kg subcutaneous (sc) METH. On day 21, this dose was repeated to determine 2 h post-METH brain concentrations. METH-induced open field behaviors were measured every other day (days 12 - 16) with ascending sc doses (0.3 – 3 mg/kg). Results: BDL transiently increased alanine aminotransferase levels and altered liver structure, which resulted in significantly greater METH serum and brain exposure. In the BDL compared to sham group, there was a longer duration of METH-induced locomotor activity (after 1 and 3 mg/kg) and stereotypy (after 3 mg/kg). Conclusions: In rats, liver dysfunction reduced METH clearance, increased brain METH concentrations, and enhanced METH effects on locomotor activity in a dose dependent manner. In addition, this model could be further developed to simulate the associated hepatic dysfunction of key METH abuse comorbidities for preclinical testing of novel pharmacotherapies for effectiveness and/or toxicity in vulnerable populations.

Mechanisms responsible for the altered pharmacokinetics of Bosentan: analysis utilizing rats with bile duct ligation-induced liver dysfunction

2009 ◽  
Vol 30 (6) ◽  
pp. 326-333 ◽  
Author(s):  
Isao Horiuchi ◽  
Yun-i Mori ◽  
Masato Taguchi ◽  
Fukiko Ichida ◽  
Toshio Miyawaki ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Dysfunction ◽  
Bile Duct Ligation ◽  
Duct Ligation

Effect of bile duct ligation-induced liver dysfunction on methamphetamine pharmacokinetics in male and female rats

2020 ◽  
Vol 215 ◽  
pp. 108190
Author(s):  
Michael D. Berquist ◽  
Mitchell R. McGill ◽  
Anna Mazur ◽  
David L. Findley ◽  
Greg Gorman ◽  
...  
Keyword(s):  
Bile Duct ◽  
Liver Dysfunction ◽  
Bile Duct Ligation ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats ◽  
Duct Ligation

Hepatoprotective and antifibrotic effects of sodium molybdate in a rat model of bile duct ligation

2015 ◽  
Vol 29 ◽  
pp. 242-248 ◽  
Author(s):  
Mahsa Ale-Ebrahim ◽  
Akram Eidi ◽  
Pejman Mortazavi ◽  
Seyyed Mohammad Tavangar ◽  
Darioush Minai Tehrani
Keyword(s):  
Bile Duct ◽  
Rat Model ◽  
Bile Duct Ligation ◽  
Sodium Molybdate ◽  
Duct Ligation

scholarly journals Bone marrow-derived mesenchymal stem cells effectively regenerate fibrotic liver in bile duct ligation rat model

2016 ◽  
Vol 241 (6) ◽  
pp. 581-591 ◽  
Author(s):  
Hoda E Mohamed ◽  
Sahar E Elswefy ◽  
Laila A Rashed ◽  
Nahla N Younis ◽  
Mohamed A Shaheen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bile Duct ◽  
Rat Model ◽  
Bile Duct Ligation ◽  
Fibrotic Liver ◽  
Duct Ligation
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