scholarly journals Effect of Pertussis Toxin and Herbimycin A on Proteinase-Activated Receptor 2-Mediated Cyclooxygenase 2 Expression inHelicobacter pylori-Infected Gastric Epithelial AGS Cells

2011 ◽  
Vol 52 (3) ◽  
pp. 522 ◽  
Author(s):  
Ji Hye Seo ◽  
Jeong Yeon Seo ◽  
Hae-Yun Chung ◽  
Hyeyoung Kim
Keyword(s):  
Pertussis Toxin ◽  
Cyclooxygenase 2 ◽  
Ags Cells ◽  
Herbimycin A

Effect of Mannitol on Helicobacter pylori-Induced Cyclooxygenase-2 Expression in Gastric Epithelial AGS Cells

Pharmacology ◽  
2002 ◽  
Vol 66 (4) ◽  
pp. 182-189 ◽  
Author(s):  
Hyeyoung Kim ◽  
Jeong Yeon Seo ◽  
Kyung Hwan Kim
Keyword(s):  
Helicobacter Pylori ◽  
Cyclooxygenase 2 ◽  
Ags Cells

scholarly journals Kinetic and biochemical correlation between sustained p44ERK1 (44 kDa extracellular signal-regulated kinase 1) activation and lysophosphatidic acid-stimulated DNA synthesis in Rat-1 cells

1996 ◽  
Vol 320 (1) ◽  
pp. 237-245 ◽  
Author(s):  
Simon J. COOKOnyx ◽  
Frank McCORMICK
Keyword(s):  
Dna Synthesis ◽  
Pertussis Toxin ◽  
Lysophosphatidic Acid ◽  
Peak Response ◽  
Erk Activation ◽  
Extracellular Signal Regulated Kinase ◽  
Herbimycin A ◽  
Extracellular Signal ◽  
Sustained Activation

Rat-1 fibroblasts were used to study the role of the sustained activation of extracellular signal-regulated kinase 1 (ERK1) in lysophosphatidic acid (LPA)-stimulated mitogenic signalling. Mitogenic doses of LPA, like serum, stimulated biphasic, sustained, ERK activation that persisted towards the G1/S boundary. The EC50 for LPA-stimulated ERK activation after 10 min, the time of peak response, was 2 orders of magnitude to the left of that for the sustained response after 3 h or that for DNA synthesis after 22 h, with the result that non-mitogenic doses stimulated a maximal peak response but no second phase. To complement these studies, we examined the role of different signal pathways in regulating the sustained and acute phases of ERK activation using defined biochemical inhibitors and mimetics. Activation of protein kinase C and Ca2+ fluxes played a minor and transient role in regulation of ERK1 activity by LPA in Rat-1 cells. Sustained ERK1 activation stimulated by LPA was completely inhibited by pertussis toxin, whereas the early peak response was only partly affected; this is correlated with the specific inhibition of LPA-stimulated DNA synthesis by pertussis toxin. The selective tyrosine kinase inhibitor herbimycin A completely inhibited sustained ERK1 activation by LPA but, again, the early phase of the response was only partially inhibited. In addition, low doses of staurosporine inhibited ERK1 activation by LPA. The effects of herbimycin A and staurosporine were selective for the response to LPA but did not affect that to epidermal growth factor. The results suggest a strong correlation between sustained ERK1 activation and DNA synthesis in LPA-stimulated Rat-1 cells. Furthermore, the two discrete phases of ERK activation by LPA are regulated by a combination of at least two different signalling pathways; the sustained activation of ERK1 in Rat-1 cells proceeds via a Gi- or Go-mediated pathway which may also involve a tyrosine kinase.

scholarly journals Ghrelin Regulates Cyclooxygenase-2 Expression and Promotes Gastric Cancer Cell Progression

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Huanqing Li ◽  
Xiaohong Zhang ◽  
Li Feng
Keyword(s):  
Gastric Cancer ◽  
Protein Expression ◽  
Gastric Cancer Cell ◽  
Molecular Targets ◽  
Cyclooxygenase 2 ◽  
Akt Inhibitor ◽  
Flow Cytometry Assay ◽  
Ags Cells ◽  
Cell Progression ◽  
Cox 2

Aim. To research the molecular mechanism of ghrelin in apoptosis, migratory, and invasion of gastric cancer (GC) cells. Methods. After GC AGS cells were handled with ghrelin (10–8 M), cyclooxygenase-2 inhibitor NS398 (100 μM), and Akt inhibitor perifosine (10uM), the rates of apoptosis were detected by TUNEL assay and flow cytometry assay. We assessed the expressions of PI3K, p-Akt, and COX-2 proteins by making use of Western blot analysis. The cell migratory and invasion were detected by using wound-healing and transwell analysis. Results. The migratory and invasion were increased in ghrelin-treated cells, while the rates of apoptosis were decreased. GC AGS cells treated with ghrelin showed an increase in protein expression of p-Akt, PI3K, and COX-2. After cells were treated with Akt inhibitor perifosine, the protein expression of p-Akt, PI3K, and COX-2 and the cell migratory, invasion, and apoptosis were partly recovered. After cells were treated with cyclooxygenase-2 inhibitor NS398, the protein expression of COX-2 and the cell migratory and invasion were decreased, while the rates of apoptosis were increased. Conclusion. Ghrelin regulates cell migration, invasion, and apoptosis in GC cells through targeting PI3K/Akt/COX-2. Ghrelin increases the expression of COX-2 in GC cells by targeting PI3K/Akt. Ghrelin is suggested to be one of the molecular targets in GC.

Contribution of stromal and epithelial cells to cyclooxygenase-2 (COX-2) activity in Barrett's esophagus

2001 ◽  
Vol 120 (5) ◽  
pp. A78-A79
Author(s):  
N BUTTAR ◽  
K WANG ◽  
M ANDERSON ◽  
L LUTZKE ◽  
K KRISHNADATH
Keyword(s):  
Epithelial Cells ◽  
Barrett’S Esophagus ◽  
Barrett's Esophagus ◽  
Cyclooxygenase 2 ◽  
Cox 2

Expressions of cyclooxygenase-2 (COX-2) and prostaglandin E receptors (EPs) in gallbladder carcinoma (GBca)-association with tumor progression

2001 ◽  
Vol 120 (5) ◽  
pp. A573-A573
Author(s):  
J SHODA ◽  
T ASANO ◽  
T KAWAMOTO ◽  
Y MATSUZAKI ◽  
N TANAKA ◽  
...  
Keyword(s):  
Tumor Progression ◽  
Gallbladder Carcinoma ◽  
Cyclooxygenase 2 ◽  
Prostaglandin E ◽  
Cox 2

Expression of survivin and its correlation with cyclooxygenase-2 in gastric cancer

2001 ◽  
Vol 120 (5) ◽  
pp. A496-A496
Author(s):  
J YU ◽  
W LEUNG ◽  
M EBERT ◽  
E NG ◽  
M GO ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Cyclooxygenase 2

Gastrin and cyclooxygenase-2 (COX-2) expression in Helicobacter pylori (Hp) infected gastric ulceration

2001 ◽  
Vol 120 (5) ◽  
pp. A727-A727
Author(s):  
S KONTUREK ◽  
P KONTUREK ◽  
W BIELANSKI ◽  
A DUDA ◽  
M ZUCHOWICZ ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Ulceration ◽  
Cyclooxygenase 2 ◽  
Cox 2

279: Inhibition of Cyclooxygenase-2 Pathway Overcomes the Heat-Resistance in Prostate Cancer Cells

2007 ◽  
Vol 177 (4S) ◽  
pp. 93-93
Author(s):  
Makoto Sumitomo ◽  
Kenji Kuroda ◽  
Takako Asano ◽  
Akio Horiguchi ◽  
Keiichi Ito ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Heat Resistance ◽  
Cancer Cells ◽  
Cyclooxygenase 2 ◽  
Prostate Cancer Cells
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