scholarly journals Inflammatory Gene Expression Patterns Revealed by DNA Microarray Analysis in TNF-α-treated SGBS Human Adipocytes

2006 ◽  
Vol 47 (5) ◽  
pp. 729 ◽  
Author(s):  
Myoung-Sool Do ◽  
Hun-Soon Jeong ◽  
Bong-Hyuk Choi ◽  
Leif Hunter ◽  
Stuart Langley ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Human Adipocytes ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene ◽  
Dna Microarray Analysis ◽  
Tnf Α

scholarly journals Model-Based Characterization of Inflammatory Gene Expression Patterns of Activated Macrophages

2016 ◽  
Vol 12 (7) ◽  
pp. e1005018 ◽  
Author(s):  
Julia Rex ◽  
Ute Albrecht ◽  
Christian Ehlting ◽  
Maria Thomas ◽  
Ulrich M. Zanger ◽  
...  
Keyword(s):  
Gene Expression ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Activated Macrophages ◽  
Inflammatory Gene Expression ◽  
Inflammatory Gene ◽  
Model Based

scholarly journals Use of the Chinchilla Model for Nasopharyngeal Colonization To Study Gene Expression by Moraxella catarrhalis

2011 ◽  
Vol 80 (3) ◽  
pp. 982-995 ◽  
Author(s):  
Todd C. Hoopman ◽  
Wei Liu ◽  
Stephanie N. Joslin ◽  
Christine Pybus ◽  
Jennifer L. Sedillo ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Moraxella Catarrhalis ◽  
Expression Patterns ◽  
Surface Proteins ◽  
Content Type ◽  
Dna Microarray Analysis ◽  
Study Gene Expression

Young adult chinchillas were atraumatically inoculated withMoraxella catarrhalisvia the nasal route. Detailed histopathologic examination of nasopharyngeal tissues isolated from theseM. catarrhalis-infected animals revealed the presence of significant inflammation within the epithelium. Absence of similar histopathologic findings in sham-inoculated animals confirmed thatM. catarrhaliswas exposed to significant host-derived factors in this environment. Twenty-four hours after inoculation, viableM. catarrhalisorganisms were recovered from the nasal cavity and nasopharynx of the animals in numbers sufficient for DNA microarray analysis. More than 100M. catarrhalisgenes were upregulatedin vivo, including open reading frames (ORFs) encoding proteins that are involved in a truncated denitrification pathway or in the oxidative stress response, as well as several putative transcriptional regulators. Additionally, 200M. catarrhalisgenes were found to be downregulated when this bacterium was introduced into the nasopharynx. These downregulated genes included ORFs encoding several well-characterizedM. catarrhalissurface proteins including Hag, McaP, and MchA1. Real-time reverse transcriptase PCR (RT-PCR) was utilized as a stringent control to validate the results ofin vivogene expression patterns as measured by DNA microarray analysis. Inactivation of one of the genes (MC ORF 1550) that was upregulatedin vivoresulted in a decrease in the ability ofM. catarrhalisto survive in the chinchilla nasopharynx over a 3-day period. This is the first evaluation of global transcriptome expression byM. catarrhaliscellsin vivo.

scholarly journals The Potential of 2-aza-8-Oxohypoxanthine as a Cosmetic Ingredient

Cosmetics ◽  
2021 ◽  
Vol 8 (3) ◽  
pp. 60
Author(s):  
Hisae Aoshima ◽  
Masayuki Ito ◽  
Rinta Ibuki ◽  
Hirokazu Kawagishi
Keyword(s):  
Gene Expression ◽  
Cell Proliferation ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Cell Activation ◽  
Skin Barrier ◽  
Efficacy Evaluation ◽  
Activation Effect ◽  
Expression Levels ◽  
Dna Microarray Analysis

In this study, we verified the effects of 2-aza-8-oxohypoxanthine (AOH) on human epidermal cell proliferation by performing DNA microarray analysis. Cell proliferation was assessed using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, which measures mitochondrial respiration in normal human epidermal keratinocyte (NHEK) cells. Gene expression levels were determined by DNA microarray analysis of 177 genes involved in skin aging and disease. AOH showed a significant increase in cell viability at concentrations between 7.8 and 31.3 μg/mL and a significant decrease at concentrations above 250 μg/mL. DNA microarray analysis showed that AOH significantly increased the gene expression of CLDN1, DSC1, DSG1, and CDH1 (E-cadherin), which are involved in intercellular adhesion and skin barrier functioning. AOH also up-regulated the expression of KLK5, KLK7, and SPIMK5, which are proteases involved in stratum corneum detachment. Furthermore, AOH significantly stimulated the expression of KRT1, KRT10, TGM1, and IVL, which are considered general differentiation indicators, and that of SPRR1B, a cornified envelope component protein. AOH exerted a cell activation effect on human epidermal cells. Since AOH did not cause cytotoxicity, it was considered that the compound had no adverse effects on the skin. In addition, it was found that AOH stimulated the expression levels of genes involved in skin barrier functioning by DNA microarray analysis. Therefore, AOH has the potential for practical use as a cosmetic ingredient. This is the first report of efficacy evaluation tests performed for AOH.

scholarly journals DNA microarray analysis of global gene regulation by A-factor in Streptomyces griseus

Microbiology ◽  
2009 ◽  
Vol 155 (7) ◽  
pp. 2197-2210 ◽  
Author(s):  
Hirofumi Hara ◽  
Yasuo Ohnishi ◽  
Sueharu Horinouchi
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Dna Microarray ◽  
Streptomyces Griseus ◽  
Exponential Growth Phase ◽  
Mobility Shift ◽  
Dna Microarray Analysis ◽  
Regulatory Cascade ◽  
Transcriptional Units ◽  
Inducible Genes

A-factor (2-isocapryloyl-3R-hydroxymethyl-γ-butyrolactone) is a microbial hormone that triggers morphological differentiation and secondary metabolism in Streptomyces griseus. The effects of A-factor on global gene expression were determined by DNA microarray analysis of transcriptomes obtained with the A-factor-deficient mutant ΔafsA. A-factor was added at a concentration of 25 ng ml−1 to mutant ΔafsA at the middle of the exponential growth phase, and RNA samples were prepared from the cells grown after A-factor addition for a further 5, 15 and 30 min, and 1, 2, 4, 8 and 12 h. The effects of A-factor on transcription of all protein-coding genes of S. griseus were evaluated by comparison of the transcriptomes with those obtained from cells grown in the absence of A-factor. Analysis of variance among the transcriptomes revealed that 477 genes, which were dispersed throughout the chromosome, were differentially expressed during the 12 h after addition of A-factor, when evaluated by specific criteria. Quality threshold clustering analysis with regard to putative polycistronic transcriptional units and levels of upregulation predicted that 152 genes belonging to 74 transcriptional units were probable A-factor-inducible genes. Competitive electrophoretic mobility shift assays using DNA fragments including putative promoter regions of these 74 transcriptional units suggested that AdpA bound 37 regions to activate 72 genes in total. Many of these A-factor-inducible genes encoded proteins of unknown function, suggesting that the A-factor regulatory cascade of S. griseus affects gene expression at a specific time point more profoundly than expected.

scholarly journals Deafness Gene Expression Patterns in the Mouse Cochlea Found by Microarray Analysis

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e92547 ◽  
Author(s):  
Hidekane Yoshimura ◽  
Yutaka Takumi ◽  
Shin-ya Nishio ◽  
Nobuyoshi Suzuki ◽  
Yoh-ichiro Iwasa ◽  
...  
Keyword(s):  
Gene Expression ◽  
Microarray Analysis ◽  
Expression Patterns ◽  
Gene Expression Patterns ◽  
Deafness Gene
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