scholarly journals Oxidative stress effect on the activation of hepatic stellate cells

2001 ◽  
Vol 42 (1) ◽  
pp. 1 ◽  
Author(s):  
Kwan Sik Lee ◽  
Se Joon Lee ◽  
Hyo Jin Park ◽  
Jun Pyo Chung ◽  
Kwang Hyub Han ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Stress Effect

scholarly journals Role of mitophagy regulation by ROS in hepatic stellate cells during acute liver failure

2018 ◽  
Vol 315 (3) ◽  
pp. G374-G384 ◽  
Author(s):  
Zhen Tian ◽  
Yi Chen ◽  
Naijuan Yao ◽  
Chunhua Hu ◽  
Yuchao Wu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Reactive Oxygen Species ◽  
Superoxide Dismutase ◽  
Liver Failure ◽  
Acute Liver Failure ◽  
Hepatic Stellate Cells ◽  
Stellate Cells ◽  
Oxygen Species ◽  
End Stage Liver Disease ◽  
End Stage

Liver sinusoids serve as the first line of defense against extrahepatic stimuli from the intestinal tract. Hepatic stellate cells (HSCs) are pericytes residing in the perisinusoidal space that integrate cytokine-mediated inflammatory responses in the sinusoids and relay these signals to the liver parenchyma. Oxidative stress has been shown to promote inflammation during acute liver failure (ALF). Whether and how oxidative stress is involved in HSC inflammation during ALF remains unclear. Level of systemic oxidative stress is reflected by superoxide dismutase (SOD). Thus, ALF patients were recruited to investigate the correlation between plasma SOD levels and clinical features. Liver tissues were collected from chronic hepatitis patients by biopsy and from ALF patients who had undergone liver transplantation. SOD2 expression and HSCs activation were investigated by immunohistochemistry. Inflammation, mitophagy, and apoptosis were investigated by immunoblot analysis and flow cytometry in HSCs treated with lipopolysaccharide (LPS) and reactive oxygen species (ROS) donors. The plasma SOD level was significantly increased in patients with ALF compared with those with cirrhosis (444.4 ± 23.58 vs. 170.07 ± 3.52 U/ml, P < 0.01) and was positively correlated with the Model for End-Stage Liver Disease-Na score ( R2 = 0.4720, P < 0.01). In vivo observations revealed that SOD2 immunostaining was increased in ALF patients and mice models, and in vitro experiments demonstrated that LPS/ROS promoted inflammation via inhibiting mitophagy. Moreover, the regulation of inflammation was apoptosis independent in HSCs. LPS-induced increases in oxidative stress promote inflammation through inhibiting mitophagy in HSCs during the process of ALF, providing a novel strategy for the treatment of patients with ALF. NEW & NOTEWORTHY Here we demonstrate that the serum superoxide dismutase (SOD) level is significantly increased in patients with acute liver failure (ALF), and, correlated with the Model for End-Stage Liver Disease-Na score, SOD level dropped in the remission stage of ALF. We identify that, in liver tissue from ALF patients and mice models, manganese-dependent SOD was overexpressed, and show lipopolysaccharide/H2O2 inhibits mitophagy via reactive oxygen species in hepatic stellate cells (HSCs). We show that inhibited mitophagy promotes inflammation in HSCs, whereas mitophagy inducer rescues HSCs from lipopolysaccharide-induced inflammation.

The Na+/H+ exchanger modulates the fibrogenic effect of oxidative stress in rat hepatic stellate cells

1999 ◽  
Vol 30 (5) ◽  
pp. 868-875 ◽  
Author(s):  
Gianluca Svegliati-Baroni ◽  
Antonio Di Sario ◽  
Alessandro Casini ◽  
Gianna Ferretti ◽  
Letizia D'Ambrosio ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Stellate Cells ◽  
Stellate Cells

scholarly journals Effects of oxidative stress on proliferation of rat hepatic stellate cells and antioxidation of reduced glutathione

2006 ◽  
Vol 14 (26) ◽  
pp. 2596
Author(s):  
Mei Liu ◽  
Lun-Gen Lu ◽  
Wei-Hua Chen ◽  
Ai-Xia Dou ◽  
Jing-Yuan Fang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Hepatic Stellate Cells ◽  
Reduced Glutathione ◽  
Stellate Cells
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